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2.7.1.158: inositol-pentakisphosphate 2-kinase

This is an abbreviated version!
For detailed information about inositol-pentakisphosphate 2-kinase, go to the full flat file.

Word Map on EC 2.7.1.158

Reaction

ATP
+
1D-myo-inositol 1,3,4,5,6-pentakisphosphate
=
ADP
 +
1D-myo-inositol hexakisphosphate

Synonyms

ASP1, At5g42810, AtIP5 2-K, AtIPK1, CnIpk1, Glyma04g03240, Glyma06g03310, GmIpk1, GmlPk1, Gsl1p, inositol 1,3,4,5,6-pentakis phosphate 2-kinase, inositol 1,3,4,5,6-pentakisphosphate 2-kinase, inositol hexakisphosphate kinase 1, inositol kinase, inositol pentakisphosphate 2-kinase, inositol polyphosphate kinase, inositol polyphosphate kinase-1, inositol polyphosphate kinase/phosphotransferase, inositol-1,3,4,5,6-pentakisphosphate 2-kinase, ins(1,3,4,5,6)P5 2-kinase, Ins5 2-kinase, InsP5 2-kinase, InsP6-kinase, IP5 2-K, IP5 2-kinase, IP5K, IP6K, IP6K1, Ipk1, Ipk1p, IPP2K, mIP5 2-K, Plc1p, rIPK1, scIpk1, spIpk1-C, StITPK1, StITPKalpha, ZmIPK1, ZmIPK1A, ZmIPK1B

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.1 Phosphotransferases with an alcohol group as acceptor
                2.7.1.158 inositol-pentakisphosphate 2-kinase

Cloned

Cloned on EC 2.7.1.158 - inositol-pentakisphosphate 2-kinase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloning and expression in Escherichia coli
Cloning from a potato tuber cDNA library and expression of StITPK1-His protein in Escherichia coli rosetta cells.
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21-AI cells
expressed in Saccharomyces cerevisiae mutant strain Scipk1DELTA
-
expression in Escherichia coli
expression in Sf21 cells. Expression of the human enzyme in yeast ipk null strain restores synthesis of 1D-myo-inositol hexakisphosphate and rescues the gle1-2 ipk1-4 lethal phenotype
-
expressionof His tagged AtIPK1 protein in Escherichia coli
-
gene Glyma04g03240, sequence comaprisons and phylogenetic tree, quantitative real-time expression analysis
gene Glyma06g03310, sequence comaprisons and phylogenetic tree, quantitative real-time expression analysis
gene GmIpk1, sequence comparisons and phylogenetic tree, quantitative real-time expression analysis
gene IPK1, DNA and amio acid sequence determination and analysis, sequence comparisons and phylogenetic tree, quantitative real-time PCR enzyme expression analysis
gene IPK1, recombinant expression
gene ipk1, recombinant expression of LSLt-tagged enzyme in Escherichia coli strain BL21 Star (DE3), and recombinant expression of GST-tagged enzyme in Spodoptera frugiperda Sf21 cells. LSLt is a fragment of Laetiporus sulphurous lectin
gene IPK1, recombinant expression of N-terminally His5-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
overexpression of EGFP-IP5K fusion proteins in NRK, COS7 and H1299 cells
-
production in Rat-1 cells
recombinant expression of His-tagged enzyme in Escherichia coli strain BL21 (Lys) DE3
spIpk1 functionally complements ipk1 mutants of Saccharomyces cerevisiae