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2.7.1.151: inositol-polyphosphate multikinase

This is an abbreviated version!
For detailed information about inositol-polyphosphate multikinase, go to the full flat file.

Word Map on EC 2.7.1.151

Reaction

ATP
+
1D-myo-inositol 1,4,5,6-tetrakisphosphate
=
ADP
+
1D-myo-inositol 1,3,4,5,6-pentakisphosphate

Synonyms

1,3,4,6-tetrakisphosphate 5-kinase, 5-kinase, Arg82, ArgRIII, ARGSIII, AtIpk2a, AtIpk2b, AtIpk2beta, HsIPMK, Impk, inositol 1,4,5-trisphosphate 3-kinase, inositol phosphate multikinase, inositol phosphate multikinase 2, inositol polyphosphate 6-/3-kinase, inositol polyphosphate kinase, inositol polyphosphate multikinase, Ins(1,4,5)P3 3-kinase, InsP4 5-kinase, IP3 3-kinase, IP3/IP4 6-/3-kinase, IP3K, IPK, Ipk2, Ipk2/Impk/IP3K, Ipk2a, Ipk2beta/IP3K, IPKII, IPMK, ITPK1, Kcs1, Kcs1p, More, phosphoinositol kinase, PI3K, StIPMK

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.1 Phosphotransferases with an alcohol group as acceptor
                2.7.1.151 inositol-polyphosphate multikinase

Purification

Purification on EC 2.7.1.151 - inositol-polyphosphate multikinase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate, Con A-Sepharose, DEAE-Toyopearl, 5'AMP-Sepharose
-
ammonuium sulfate, DEAE-cellulose
-
native enzyme partially by nuclear fraction preparation
recombinant enzyme
-
recombinant enzyme from Escherichia coli strain BL21(DE3) to near homogeneity by ion exchange and phosphocellulose resin chromatography
recombinant enzyme from strain expression in strain 12S16cpep4D by nickel affinity chromatography
-
recombinant GST-fusion isozymes from Escherichia coli by glutathione affinity chromatography
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli by nickel affinity and heparin affinity chromatography, followed by tag cleavage through TEV protease, another step of heparin affinity chromatography, and gel filtration
recombinant N-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3) by cobalt affinity chromatography
-
recombinant wild-type enzyme and deletion mutant from Escherichia coli by ion exchange and phosphocellulose resin chromatography
-