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2.7.1.151: inositol-polyphosphate multikinase

This is an abbreviated version!
For detailed information about inositol-polyphosphate multikinase, go to the full flat file.

Word Map on EC 2.7.1.151

Reaction

ATP
+
1D-myo-inositol 1,4,5,6-tetrakisphosphate
=
ADP
 +
1D-myo-inositol 1,3,4,5,6-pentakisphosphate

Synonyms

1,3,4,6-tetrakisphosphate 5-kinase, 5-kinase, Arg82, ArgRIII, ARGSIII, AtIpk2a, AtIpk2b, AtIpk2beta, HsIPMK, Impk, inositol 1,4,5-trisphosphate 3-kinase, inositol phosphate multikinase, inositol phosphate multikinase 2, inositol polyphosphate 6-/3-kinase, inositol polyphosphate kinase, inositol polyphosphate multikinase, Ins(1,4,5)P3 3-kinase, InsP4 5-kinase, IP3 3-kinase, IP3/IP4 6-/3-kinase, IP3K, IPK, Ipk2, Ipk2/Impk/IP3K, Ipk2a, Ipk2beta/IP3K, IPKII, IPMK, ITPK1, Kcs1, Kcs1p, More, phosphoinositol kinase, PI3K, StIPMK

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.1 Phosphotransferases with an alcohol group as acceptor
                2.7.1.151 inositol-polyphosphate multikinase

Cloned

Cloned on EC 2.7.1.151 - inositol-polyphosphate multikinase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis, no functional complementation of yeast mutant strains, expression of the N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3)
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DNA and amino acid sequence determination and analysis, overexpression as C-terminally GFP-tagged fusion protein in Escherichia coli strain BL21(DE3), transient expression in NRK 52E cells as C- or N-terminally GFP-tagged fusion protein
Examinations of plants deficient in IPMK suggest roles in pollen germination, root growth, as well axillary shoot branching.
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expressed in Lycopersicon esculentum cv. PKM
expression in bacteria as glutathione S-transferase fusion protein
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expression in enzyme-deficient yeast cells, stable overexpression in HEK-293 cells, abrogates the in vivo elevation of 1D-myo-inositol 1,4,5,6-tetrakisphosphate from the Salmonella dublin SopB protein
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expression in HEK293 cells as glutathione S-transferase fusion protein
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expression in Saccharomyces cerevisiae. A lysine accumulation phenotype of ScIpk2DELTA mutant yeast is rescued by Solanum tuberosum inositol phosphate multikinase
Expression of AtIpk2b in yeast mutant arg82D, quantitative real-time PCR analysis of AtIpk2b expression in Arabidopsis. AtIpk2b cDNA containing the complete open reading frame is inserted via XbaI-SalI sites into the binary vector pBinAR-HPT. Resulting construct is introduced into Agrobacterium tumefaciens (strain LBA4404). Tobacco leaf disks are infected with the transformed Agrobacteria.
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expression of HA-tagged enzyme in human HEK293 and in COS-7 cells
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expression of wild-type enzyme and deletion mutant in Escherichia coli
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gene impk, adeno-associated virus serotype 2-mediated delivery of IPMK into mouse brain, in the striatum of R6/2 Huntington's disease mice
gene impk, recombinant GST-tagged IMPK overexpression in human colon cancer cell line HCT-116 and the human osteosarcoma cell line U2-OS. Overexpression of kinase-deficient IPMK inhibits cell proliferation in etoposide-treated U2OS cells to a similar extent as does wild-type IPMK
gene ipk2 is located on chromosome 10
-
gene ipmk, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli
gene KCS1, subcloning in Escherichia coli strain XL1-B, overexpression in strain arg82DELTA, expression in strain 12S16cpep4D
-
genetic map of inositol metabolism, overexpression of isozyme Kcs1 can complement isozyme Ipk2 deficiency
-
His-tagged enzyme, expression in Escherichia coli
IPMK knockout mice demonstrates the critical roles of IPMK in embryogenesis and central nevous system development. Deletion of IPMK in mice results in early lethality.
isozyme Ipk2alpha, location on chromosome 5, DNA and amino acid sequence determination and analysis, expression of wild-type and mutant isozyme in Escherichia coli with or without GST-fusion tag, functional complementation of an enzyme-deficient yeast strain
isozyme Ipk2beta, location on chromosome 5, DNA and amino acid sequence determination and analysis, expression of wild-type and mutant isozyme in Escherichia coli with or without GST-fusion tag, functional complementation of an enzyme-deficient yeast strain
Observed derepression and constitutive elevated levels levels of the transcripts in IPMK mutant yeast but not in ipk1- or ipk6-deficient yeasts confirms the requirement of IPMK in the transcriptional regulation of arginine anabolism and catabolism.
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overexpressed in Escherichia coli strain BL21Star
-
To create an AtIpk2b overexpression construct, the AtIpk2b cDNA is cut from plasmid pmIP3K with XbaI-XhoI and inserted via XbaI-SalI sites into pBinAR-HPT vector behind the cauliflower mosaic virus 35S promoter. The resulting plasmid is first introduced into Agrobacterium tumefaciens by electroporation and then transformed Arabidopsis ecotype C24 by vacuum infiltration. Transgenic plants are selected on MS medium containing 20 mg/L hygromycin. Eleven AtIpk2b overexpression plants, named OX-2, OX-3, OX-5, OX-8, OX-9, OX-15, OX-25, OX-26, OX-33, OX-35, and OX-64, are identified. Compared to the wild type, branching of axillary shoots is more pronounced in the overexpression lines.
While wild-type yeast displays sequential phosphorylation of Ins(1,4,5)P3 to Ins6, mutation of locus termed GSL3 resulted in the loss of Ins(1,4,5)P3-initiated higher isnositol polyphosphate metabolism. IPMK is identified as GSL3 (Ipk2) and confirmed its requirement for mRNA export.
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