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2.7.1.148: 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase

This is an abbreviated version!
For detailed information about 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase, go to the full flat file.

Word Map on EC 2.7.1.148

Reaction

ATP
+
4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
=
ADP
 +
2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol

Synonyms

4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase, 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase, 4-(cytidine-5-diphospho)-2-C-methyl-D-erythritol kinase, 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase, 4-diphosphocytidyl-2-C-methylerythritol 2-kinase, 4-diphosphocytidyl-2C-methyl-D-erythritol 2-kinase, 4-diphosphocytidyl-2C-methyl-D-erythritol kinase, AaIspE, BsIspE, CcCMK1, CDP-ME, CDP-ME kinase, CDP-methylerythritol kinase, CDPME kinase, CDPMEK, CMK, EcIspE, GbCMK1, GbCMK2, IspDF, IspE, More, PvIspE, Ripening-associated protein pTOM41, TwCMK, YchB

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.1 Phosphotransferases with an alcohol group as acceptor
                2.7.1.148 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase

Cloned

Cloned on EC 2.7.1.148 - 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene ispE, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, genotyping. Multiple sequence analysis (MSA) of deduced amino acid sequences of PvIspE gene reveals that PvIspE amino acid sequences are regionally conserved as they show 100% similarity among all Indian isolates from a particular region. Specific mutations are found in different regions
gene ispE, expression of the His-tagged in strain BL21(DE3)
-
gene ispE, recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) from pGEX-6P-1 expression vector
gene ispE, recombinant expression of GST-tagged enzyme in Escherichia coli strain DH5alpha from pGEX-6P-1 expression vector
gene TwCMK, DNA and amino acid sequence determination and analysis, realtime fluorescence quantification PCR, RT-qPCR, expression analysis
-
gene ychB, expression of the selenomethionine-labeled enzyme in Escherichia coli strain B834(DE3)
-
gene ychB, overexpression of His6-tagged enzyme
-
overexpression in Escherichia coli
-
putative catalytic domain, residues 81-401
The putative ispE gene from Aquifex aeolicus is placed under the control of the T7 promoter and lac operator in the hyperexpression plasmid pET-15b, and heat transformed into Escherichia coli BL21 (DE3).
The two CMK sequences encoding the mature CMK proteins are amplified with their respective primer pairs and cloned to the pMW118 vector to derive the pMW-CMK1 and the pMW-CMK2 plasmids. NMW29, an Escherichia coli ychB mutant harboring the pTMV20KM plasmid. The strain is transformed with the pMW-CMK1 and the pMW-CMK2 plasmids and selected on the LB medium without mevalonate.
transcriptome analysis, CcCMK1 DNA and amino acid sequence determination and analysis, real-time PCR enzyme expression analysis