2.7.1.147: ADP-specific glucose/glucosamine kinase

This is an abbreviated version!
For detailed information about ADP-specific glucose/glucosamine kinase, go to the full flat file.

Word Map on EC 2.7.1.147

2.7.1.147

hyperthermophilic

thermococcus

embden-meyerhof

litoralis

phosphofructokinases

jannaschii

furiosus

glucokinases

ribokinase

methanococcales

thermococcales

6-phosphofructokinase

hexokinases

atp-pfks

methanosarcinales

Reaction

Synonyms

ADP-dependent glucokinase, ADP-dependent hexokinase, ADP-dependent kinase, ADP-GK, ADP-HK, ADP-Pfk, ADP-specific glucokinase, ADP:D-glucose 6-phosphotransferase, ADPGK, ancGK/PFK, AncMsPFK/GK, bifunctional ADP-dependent phosphofructokinase/glucokinase, GlcN kinase, glucosamine kinase, MevePFK/GK, MjPFK/GK, MmazPFK/GK, MmPFK/GK, More, NagC4, pfGK, pfkC, PhPFK, TK1110, tlGK

ECTree

2 Transferases

2.7 Transferring phosphorus-containing groups

2.7.1 Phosphotransferases with an alcohol group as acceptor

2.7.1.147 ADP-specific glucose/glucosamine kinase

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Results	in table
4	Activating Compound
195	AA Sequence
1	CAS Registry Number
27	Cloned(Commentary)
12	Cofactor
15	Crystallization (Commentary)
26	Disease/ Diagnostics
62	General Information
2	General Stability
3	IC50 Value
34	Inhibitors
43	kcat/KM [mM/s]
28	Ki Value [mM]
71	KM Value [mM]
4	Localization
77	Metals/Ions
14	Molecular Weight [Da]
50	Natural Substrates/ Products (Substrates)
144	Organism
1	Oxidation Stability
8	Pathway
16	PDB ID
22	pH Optimum
4	pH Range
24	Protein Variants
24	Purification (Commentary)
3	Reaction
1	Reaction Type
75	Reference
28	Source Tissue
13	Specific Activity [micromol/min/mg]
138	Substrates and Products (Substrate)
12	Subunits
141	Synonyms
1	Systematic Name
18	Temperature Optimum [°C]
7	Temperature Stability [°C]
42	Turnover Number [1/s]

Purification

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Purification on EC 2.7.1.147 - ADP-specific glucose/glucosamine kinase

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12 entries

600fold to homogeneity by ultracentrifugation, ion exchange chromatography, ammonium sulfate fractionation, hydrophobic interaction chromatography, gel filtration, hydroxyapatite chromatography, and 2 additional ion exchange chromatographic steps

Archaeoglobus fulgidus

660831

recombinant enzyme from Escherichia coli strain BL21(DE3) by heat treatment at 90°C, ammonium sulfate fractionation, ion exchange chromatography, and gel filtration

Pyrococcus furiosus

Q9V2Z6

662610

recombinant enzyme from Escherichia coli strain DH5alpha by nickel affinity and ion exchange chromatography, to homogeneity

Mus musculus

Q8VDL4

660867

recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, dialysis, and anion exchange chromatography

3 entries

recombinant N-terminally His-tagged enzyme from Escherichia coli by heat treatment and nickel affinity chromatography

Pyrococcus horikoshii

663324

recombinant N-terminally His6-tagged enzyme from Escherichia coli strains BL21(DE3) and BL21-C41(DE3) by heat treatment at 50°C for 30 min, nickel affinity chromatography, dialysis, and anion exchange chromatography

Methanohalobium evestigatum

D7E8P3

760433

recombinant N-terminally His6-tagged enzyme from Escherichia coli strains BL21(DE3) and BL21-C41(DE3) by nickel affinity chromatography, and dialysis

Methanococcoides burtonii

Q12WB9

760433

Resource Q column chromatography and Superdex S200 gel filtration

Thermococcus kodakarensis

Q5JE39

759521