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2.7.1.127: inositol-trisphosphate 3-kinase

This is an abbreviated version!
For detailed information about inositol-trisphosphate 3-kinase, go to the full flat file.

Word Map on EC 2.7.1.127

Reaction

ATP
+
1D-myo-inositol 1,4,5-trisphosphate
=
ADP
 +
1D-myo-inositol 1,3,4,5-tetrakisphosphate

Synonyms

1D-myo-inositol-trisphosphate 3-kinase, Arg82, ArgRIII, D-myo-inositol 1,4,5-trisphosphate 3-kinase, GsIP3K-A, HsIP3K-A, inositol (1,4,5) trisphosphate 3 kinase B, inositol (1,4,5) trisphosphate 3-kinase, inositol 1,4,5-trisphosphate 3-kinase, inositol 1,4,5-trisphosphate 3-kinase A, inositol 1,4,5-trisphosphate 3-kinase B, inositol 1,4,5-trisphosphate 3-kinase C, inositol 1,4,5-trisphosphate 3-kinases, inositol 1,4,5-trisphosphate kinase, inositol 1,4,5-trisphosphate kinase 2, inositol 1,4,5-trisphosphate-3-kinase-A, inositol polyphosphate multikinase, inositol trisphosphate 3-kinase B, inositol(1,4,5)P3 3-kinase, inositol(1,4,5)trisphosphate 3-kinase, inositol(1,4,5)trisphosphate 3-kinases, inositol-1,4,5-trisphosphate 3-kinase, inositol-1,4,5-trisphosphate 3-kinase A, inositol-1,4,5-trisphosphate 3-kinase-A, inositol-1,4,5-trisphosphate-3-kinase, inositol-1,4,5-trisphosphate-3-kinase A, inositol-1,4,5-trisphosphate-3-kinase-A, inositol-trisphosphate 3-kinase B, Ins(1,4,5)P(3) 3-kinase B, Ins(1,4,5)P3 3-kinase, Ins(1,4,5)P3 3-kinase isoform B, Ins(1,4,5)P3 kinase, InsP3 3-kinase, InsP3 3-kinase A, InsP3 3-kinase B, InsP3 3-kinase C, InsP3K, InsP3KB, InsP3Kinase, IP3 3-kinase, IP3 3-kinase 2, IP3-3K, IP3K, IP3K-A, IP3K-B, IP3K-C, IP3K1, IP3K2, IP3KA, IP3KB, IP3KC, IP3kin, Ipk2, IPMK, ITPKA, Itpkb, Itpkc, kinase (phosphorylating), inositol 1,4,5-trisphosphate 3-, More, RnIP3K-C, wavy

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.1 Phosphotransferases with an alcohol group as acceptor
                2.7.1.127 inositol-trisphosphate 3-kinase

Expression

Expression on EC 2.7.1.127 - inositol-trisphosphate 3-kinase

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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
cancer cell lines show large differences in their enzyme levels: whereas SKBR-3 and T47D cells expressed very low enzyme levels, very high expression levels are found in the highly metastatic cell lines H1299, MDA231, and HepG2, most of the transformed cell lines (H1299, MDA231, Mevo, Jurkat T-cells, HEK293, HepG2) examined express increased levels of ITPKA compared to the low levels in non-transformed cells (IMR-90, HmEpc, HSF-14, leucocytes)
H1299 control and H1299 cells overexpressing inositol 1,4,5-trisphosphate-3-kinase-A (H1299-ITPKA) cells are injected subcutaneously in BALB/c severe combined immunodeficient SCID mice, high expression of ITPKA increases invasive migration in vitro and metastasis in a xenograft SCID mouse model, high expression of ITPKA during invasion of tumour cells from the primary tumour to the neighbouring tissue
in lung and breast cancer expression of ITPKA is stimulated by gene body methylation, ITPKA gene body methylation occurs early in tumor development
IP3K2 expression and Ca2+ entry are upregulated in osteosarcoma cells following treatment with chemotherapeutic drugs, e.g. doxorubicin and cisplatin
overexpression of IP3K-A in DIV 22 hippocampal neurons by infection with adenovirus containing full-length inositol 1,4,5-trisphosphate 3-kinase A leads to a markedly increased number of dendritic structure 18 h after infection, overexpressed GFP-IP3K-A signals are highly localized in the heads of dendritic protrusions
phenotypic consequences of enzyme expression in tumour cells: enzyme is stably knocked-downed in 2 cell lines with high enzyme expression levels (MDA231, H1299) and is stably overexpressed in low enzyme expressing A549 cells. MDA231, H1299 and A549-ITPKA cells show dramatically changed phenotypes compared with their respective control cells: highly metastatic MDA231 control cells migrate completely through the matrix and grow in an evenly scattered pattern, knock down variants grow as cell clusters inside the gelatin layer or adhered to the bottom of the culture dish in tight aggregates. Also H1299 control cells completely migrate through the gelatin. H1299 kd cells by contrast remain inside the gelatin matrix, forming tight aggregates of round cells. In cells with up-regulated enzyme expression, the opposite behaviour as in cells with low enzyme expression is observed. Most of the A549-ITPKA cells migrate through the gelatin layer and adhered to the bottom of the culture dish. Fewer A549 control cells migrated through the gelatin. Non-migrating cells remain in the matrix and cell clusters have a rounded morphology. To analyze the influence of enzyme on invasive migration, transwell migration assays are performed. Transwell migration of MDA231 kd cells is reduced by 73%, and that of H1299 kd cells by 58%, as compared to the respective control cells exhibiting high levels of enzyme. Approximately twice as many A549-ITPKA cells penetrate as A549 control cells (101%). A549-ITPKA cells show 2.5fold higher migration compared to control A549 cells, while migration of H1299 kd cells is reduced 3.4fold relative to H1299 control cells
the microRNA miR-140-5p inhibits IP3k2 expression in osteosarcoma cells via predicted 3'-UTR target sites and mRNA destabilization. Inhibiting IP3k2 protein expression promotes autophagy in response to anticancer drug treatment
Western blot analysis reveals that nontransformed cells express low (IMR-90, HSF-14) to nearly undetectable (HmEpc, leucocytes) levels of ITPKA