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2.7.1.127: inositol-trisphosphate 3-kinase

This is an abbreviated version!
For detailed information about inositol-trisphosphate 3-kinase, go to the full flat file.

Word Map on EC 2.7.1.127

Reaction

ATP
+
1D-myo-inositol 1,4,5-trisphosphate
=
ADP
 +
1D-myo-inositol 1,3,4,5-tetrakisphosphate

Synonyms

1D-myo-inositol-trisphosphate 3-kinase, Arg82, ArgRIII, D-myo-inositol 1,4,5-trisphosphate 3-kinase, GsIP3K-A, HsIP3K-A, inositol (1,4,5) trisphosphate 3 kinase B, inositol (1,4,5) trisphosphate 3-kinase, inositol 1,4,5-trisphosphate 3-kinase, inositol 1,4,5-trisphosphate 3-kinase A, inositol 1,4,5-trisphosphate 3-kinase B, inositol 1,4,5-trisphosphate 3-kinase C, inositol 1,4,5-trisphosphate 3-kinases, inositol 1,4,5-trisphosphate kinase, inositol 1,4,5-trisphosphate kinase 2, inositol 1,4,5-trisphosphate-3-kinase-A, inositol polyphosphate multikinase, inositol trisphosphate 3-kinase B, inositol(1,4,5)P3 3-kinase, inositol(1,4,5)trisphosphate 3-kinase, inositol(1,4,5)trisphosphate 3-kinases, inositol-1,4,5-trisphosphate 3-kinase, inositol-1,4,5-trisphosphate 3-kinase A, inositol-1,4,5-trisphosphate 3-kinase-A, inositol-1,4,5-trisphosphate-3-kinase, inositol-1,4,5-trisphosphate-3-kinase A, inositol-1,4,5-trisphosphate-3-kinase-A, inositol-trisphosphate 3-kinase B, Ins(1,4,5)P(3) 3-kinase B, Ins(1,4,5)P3 3-kinase, Ins(1,4,5)P3 3-kinase isoform B, Ins(1,4,5)P3 kinase, InsP3 3-kinase, InsP3 3-kinase A, InsP3 3-kinase B, InsP3 3-kinase C, InsP3K, InsP3KB, InsP3Kinase, IP3 3-kinase, IP3 3-kinase 2, IP3-3K, IP3K, IP3K-A, IP3K-B, IP3K-C, IP3K1, IP3K2, IP3KA, IP3KB, IP3KC, IP3kin, Ipk2, IPMK, ITPKA, Itpkb, Itpkc, kinase (phosphorylating), inositol 1,4,5-trisphosphate 3-, More, RnIP3K-C, wavy

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.1 Phosphotransferases with an alcohol group as acceptor
                2.7.1.127 inositol-trisphosphate 3-kinase

Cloned

Cloned on EC 2.7.1.127 - inositol-trisphosphate 3-kinase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
a cDNA encoding InsP3 3-kinase B is cloned from a thymus cDNA library and expressed in Escherichia coli, sequence of the 673 amino acids protein
-
cDNA encoding InsP3 3-kinase B is cloned from a hippocampus cDNA library and expressed in Escherichia coli, amino acid sequence
-
cDNAs encoding enzyme isoforms A from brain and B are cloned and overexpressed in B31 cells, a rat-1 fibroblast cell line expressing pp60v-src
-
Cloning and expression of isoform A and of a fragment comprising the catalytic calmodulin binding domain (amino acids 165-462) in Escherichia coli BL21(DE3).
Cloning and expression of isoform A and of an enzyme fragment comprising the catalytic calmodulin binding domain in Escherichia coli BL21(DE3). Creating of a point mutant by using PCR-based site-directed QuikChange mutagenesis and expression in Escherichia coli.
-
Cloning and expression of isoform B and of a fragment comprising the catalytic calmodulin binding domain (amino acids 165-462) in Escherichia coli BL21(DE3).
cloning and expression of the isoenzymes A, B and C in COS-7 cells, the 3 isoenzymes A, B and C can be distinguished by their localization on transfection in COS-7 cells
-
cloning of a full-length cDNA encoding IP3K isoform C from tongue epithelium, cloning of an enzymatically active and Ca2+/calmodulin-regulated fragment of isoform C and expression in Escherichia coli BL21(DE3)pRIL
-
cloning of isozyme IP3K-A from erythrocytes and expression of a fragment comprising the catalytic domain and the CaM binding domain of the wild-type and mutant enzyme, respectively, in Escherichia coli strain BL21(DE3)
-
Cloning, Expression, and Purification of RnIP3K-C isoform and of two different recombinant fragments IP3K-C, one comprising the catalytic domain and the calmodulin binding domain, the other comprising only the catalytic domain, are expressed in Escherichi coli BL21(DE3) cells.
entire gene encoding IP3K is cloned and 130fold overexpressed in Escherichia coli BL21(DE3), when induced by isopropyl-beta-D-thiogalactoside
-
establishment of A549 or H1299 cells overexpressing the enzyme: the cDNA fragment encoding for ITPKAis cloned into the retroviral vector MigRI, for virus production this construct is co-transfected with SVgp (M57) and pHCMV-VSV-G (M75) into HEK293 phoenix ampho cells, after 48 h of incubation the supernatant containing the viruses is filtered and transferred to A549 or H1299 cells
expression of full length enzyme in Escherichia coli: a cDNA fragment encoding the full length form of ITPKA is inserted into the vector pGEX-4T-3 encoding a N-terminal Glutathione S-transferase-tag
expression of HA-tagged enzyme in human HEK293 and in COS-7 cells
-
expression of His6-tagged fragment comprising residues 151-461 in Escherichia coli, expression of wild-type and mutant cDNA fragments encoding for residues 187-461 of the catalytic domain of isozyme A as unlabeled or selenomethionine-labeled GST fusion proteins in Escherichia coli strains C41(DE3) and B834(DE3)
-
expression of isozymes IP3-3KA, IP3-3KB, and IP3-3KC in COS-7 cells
-
expression of mutant cDNA fragment encoding for residues 185-459 of the catalytic domain of isozyme A as unlabeled or selenomethionine-labeled GST fusion proteins in Escherichia coli
-
for stable knock-down of enzyme expression in MDA231 and H1299 cells a lentiviral transduction approach is employed
full-length and truncated isoenzyme A is cloned and expressed in HeLa cells, COS-7 cells and primary neuronal cultures
-
fusion protein of HsIP3K-C with an N-terminal EGFP tag (EGFP/HsIP3KC) is transiently expressed in NRK52E cells
gene IP3k2, quantitative real-time PCR expression analysis
gene Itpka
gene Itpka, quantitative real-time PCR expression analysis
gene Itpka, quantitative reverse transcription-PCR enzyme expression analysis
gene ITPKA, recombinant expression in Escherichia coli strain BL21
gene ITPKA, recombinnant expression of N-terminally GST-tagged isozyme ITPKA in Escherichia coli strain BL21 C41lys
gene Itpkb
gene Itpkc
gene Itpkc, quantitative real-time PCR expression analysis, recombinant expression in human HEK-293 cells
gene wavy, DNA and amino acid sequence determination and analysis, detailed analysis of genetic organization, overview
generation of knock-out mice
-
generation of knock-out rats and point mutant, IP3K-A is overexpressed in DIV 22 hippocampal neurons by infection with adenovirus containing full-length IP3K-A
inositol 1,4,5-trisphosphate 3-kinase B-deficient mice are crossed to transgenic mice that express a B cell receptor specific for hen egg lysozyme
-
IP3K is overexpressed in Escherichia coli
-
isoenzyme A is cloned and overexpressed in CHO cells
-
isoform B is cloned and expressed in pTet-Off-transfected HeLa cells
-
isozymes IP3K-A and IP3K-B, expression of a fragment comprising the catalytic domain and the CaM binding domain, residues 165-462 of isozyme A, of the wild-type and mutant isozyme A and isozyme B, respectively, in Escherichia coli strain BL21(DE3)
-
overexpression of enzyme D-IP3K1 leads to increased resistance of flies to H2O2, but not to paraquat-induced oxidative stress, due to a decreased 1D-myo-inositol 1,4,5-trisphosphate level
-
stable expression of the C-terminal catalytic domain 3KA-cat30, comprising residues Ser185-Arg459, as N-terminally His-tagged and S-tagged protein in Escherichia coli strain BL21(DE3)
-
transient expression of isozyme B fragment 108-170 fused to EGFP in NRK 52E and PC12 cells co-localizes with F-actin, overexpression of isozyme B fused to EGFP in Escherichia coli, the enzyme co-localizes in with the cytoskeleton as well as with the endoplasmic reticulum and the plasma membrane, overexpression of inactive isozyme B lacking the F-actin target sequence at the noncatalytical N-terminus
-
transient expression of isozyme B in NRK 52E and PC12 cells, DNA and amino acid sequence determination and analysis, expression of isoform B comprising residues 555-934 as Strep-Tactin-tagged enzyme in Escherichia coli strain XL1-Blue, expression of isoform B comprising residues 108-170 as GST-fusion protein in Escherichia coli strain BL21(DE3)