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2.7.1.119: hygromycin-B 7''-O-kinase

This is an abbreviated version!
For detailed information about hygromycin-B 7''-O-kinase, go to the full flat file.

Word Map on EC 2.7.1.119

Reaction

ATP
+
hygromycin B
=
ADP
 +
7''-O-phosphohygromycin B

Synonyms

HPH, HPT, hygromycin B phosphotransferase, hygromycin phosphotransferase, phosphotransferase, hygromycin B

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.1 Phosphotransferases with an alcohol group as acceptor
                2.7.1.119 hygromycin-B 7''-O-kinase

Engineering

Engineering on EC 2.7.1.119 - hygromycin-B 7''-O-kinase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
L79F/S83L/G189R/E237K/S252P/D285N/L290M
random mutagenesis, mutant HYG10 contains seven substitutions and a duplication, it is thermostabilized compared to the wild-type enzyme, three-dimensional structure analysis, overview. Substitutions G189R, E237K, and D285N are located at the protein surface. The mutant shows altered kinetics at 45°C compared to the wild-type enzyme
additional information
-
use the hygromycin phosphotransferase gene (hpt) as a selective marker gene for tracking plastid transformation in rice. A site-specific transformation vector of rice plastid, pCTE04, is constructed for dicistronic expression of the hygromycin phosphotransferase (hpt) and enhanced green fluorescent protein genes (EGFP). Transplastomic rice plants successfully grow up after a gradual selection of hygromycin resistance. Integration of the dicistronic expression cassette into the intergenic region trnI-trnA of plastid genome is verified by Southern blotting analysis. Transplastomic expression of the hpt and EGFP genes is identified by RT-PCR. The stable expression of dicistronic cassette in the rice is confirmed by confocal microscopy