2.7.1.119: hygromycin-B 7''-O-kinase

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For detailed information about hygromycin-B 7''-O-kinase, go to the full flat file.

Word Map on EC 2.7.1.119

2.7.1.119

agrobacterium

tumefaciens

agrobacterium-mediated

protoplast

neomycin

beta-glucuronidase

tumefaciens-mediated

hygromycin-resistant

calli

co-cultivation

nidulans

embryogenic

bombard

acetosyringone

co-transformation

hptii

camv35s

biolistic

fescue

b-resistant

marker-free

nopaline

hygroscopicus

microprojectile

arundinacea

festucae

peg-mediated

phosphinothricin

molecular biology

biotechnology

Reaction

Synonyms

HPH, HPT, hygromycin B phosphotransferase, hygromycin phosphotransferase, phosphotransferase, hygromycin B

ECTree

2 Transferases

2.7 Transferring phosphorus-containing groups

2.7.1 Phosphotransferases with an alcohol group as acceptor

2.7.1.119 hygromycin-B 7''-O-kinase

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Results	in table
28	AA Sequence
2	Application
1	CAS Registry Number
2	Cloned(Commentary)
1	Cofactor
1	General Stability
3	Inhibitors
4	kcat/KM [mM/s]
2	Ki Value [mM]
9	KM Value [mM]
1	Localization
3	Metals/Ions
3	Molecular Weight [Da]
3	Natural Substrates/ Products (Substrates)
4	Organism
2	PDB ID
2	pH Optimum
1	pH Range
2	Protein Variants
3	Purification (Commentary)
1	Reaction
1	Reaction Type
4	Reference
2	Specific Activity [micromol/min/mg]
1	Storage Stability
5	Substrates and Products (Substrate)
2	Subunits
7	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]
2	Temperature Stability [°C]
4	Turnover Number [1/s]

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Cloned on EC 2.7.1.119 - hygromycin-B 7''-O-kinase

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gene hyg, subcloning in Escherichia coli strain JM109, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3), recombinant enzyme expression from plasmid pT8S-P31 in Thermus thermophilus strain B27 TH104 providing hygromycin B resistance up to 60°C, while recombinant expression of a mutant variant HYG10, containing seven substitutions and a duplication, provides HgB resistance up to 74°C

Streptomyces hygroscopicus

P09979

737861

recombinant gene expression in transgenic Oryza sativa from site-specific transformation vector of rice plastid, pCTE04

Streptomyces sp.

761965