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2.7.1.107: diacylglycerol kinase (ATP)

This is an abbreviated version!
For detailed information about diacylglycerol kinase (ATP), go to the full flat file.

Word Map on EC 2.7.1.107

Reaction

ATP
+
1,2-diacyl-sn-glycerol
=
ADP
 +
1,2-diacyl-sn-glycerol 3-phosphate

Synonyms

1,2-diacylglycerol kinase, 1,2-diacylglycerol kinase epsilon, adenosine 5'-triphosphate:1,2-diacylglycerol 3-phosphotransferase, arachidonoyl-specific diacylglycerol kinase, ATP:diacylglycerol phosphotransferase, DAG kinase, DAG-kinase, DAGK, DAGKalpha, DAGKepsilon, DG kinase, DGK, DGK zeta, DGK-1, DGK-3, DGK-3 diacylglycerol kinase, DGK-alpha, DGK-theta, DGK-zeta, DGK1, DGK2, DGK3, DGK4, DGK5, DGK6, DGK7, DGKA, DGKalpha, DgkB, DGKbeta, DGKdelta, DGKE, DGKepsilon, DGKeta, DGKeta1, DGKeta2, DGKeta3, DGKeta4, DGKgamma, DGKiota, DGKkappa, DGKksi, DGKtheta, DGKzeta, diacylglycerol kinase, diacylglycerol kinase (ATP dependent), diacylglycerol kinase 4, diacylglycerol kinase alpha, diacylglycerol kinase beta, diacylglycerol kinase delta, diacylglycerol kinase epsilon, diacylglycerol kinase eta, diacylglycerol kinase theta, diacylglycerol kinase zeta, diacylglycerol kinase-alpha, diacylglycerol kinase-epsilon, diacylglycerol kinase-zeta, diacylglycerol:ATP kinase, diglyceride kinase, epsilonDGK, eye-specific diacylglycerol kinase, kinase (phosphorylating), 1,2-diacylglycerol, kinase, 1,2-diacylglycerol (phosphorylating), OsDAGK1, Rv2252, sn-1,2-diacylglycerol kinase, type V DGK, YegS, YerQ

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.1 Phosphotransferases with an alcohol group as acceptor
                2.7.1.107 diacylglycerol kinase (ATP)

Temperature Stability

Temperature Stability on EC 2.7.1.107 - diacylglycerol kinase (ATP)

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25
recombinant purified isoform DGKepsilon and DGKepsilonDELTA40 are largely inactivated after being stored at room temperature for 7.5 h. After incubation at room temperature for 2 h, the proportions of activity that isoforms DGKepsilon and DGKepsilonDELTA40 retain are 35.2 and 50.3%, respectively
4
after 6.5 h at 4°C, isoform DGKepsilon and DGKepsilonDELTA40 retain 19.1% and 15.8% of their initial activities, respectively, and 48.2% and 38.1% when stored in 50% glycerol, respectively
42
-
5 min, complete loss of activity of DGK-II and DGK-III, about 10% loss of activity of enzyme form DGK-I
43 - 45
-
preincubation of microtubules, 10 min, 50% loss of activity
57
-
Triton-solubilized preparation, t1/2: 12 min
additional information
-
addition of phospholipids provides some protection from thermal inactivation