2.7.1.107: diacylglycerol kinase (ATP)

This is an abbreviated version!
For detailed information about diacylglycerol kinase (ATP), go to the full flat file.

Reaction

Synonyms

1,2-diacylglycerol kinase, 1,2-diacylglycerol kinase epsilon, adenosine 5'-triphosphate:1,2-diacylglycerol 3-phosphotransferase, arachidonoyl-specific diacylglycerol kinase, ATP:diacylglycerol phosphotransferase, DAG kinase, DAG-kinase, DAGK, DAGKalpha, DAGKepsilon, DG kinase, DGK, DGK zeta, DGK-1, DGK-3, DGK-3 diacylglycerol kinase, DGK-alpha, DGK-theta, DGK-zeta, DGK1, DGK2, DGK3, DGK4, DGK5, DGK6, DGK7, DGKA, DGKalpha, DgkB, DGKbeta, DGKdelta, DGKE, DGKepsilon, DGKeta, DGKeta1, DGKeta2, DGKeta3, DGKeta4, DGKgamma, DGKiota, DGKkappa, DGKksi, DGKtheta, DGKzeta, diacylglycerol kinase, diacylglycerol kinase (ATP dependent), diacylglycerol kinase 4, diacylglycerol kinase alpha, diacylglycerol kinase beta, diacylglycerol kinase delta, diacylglycerol kinase epsilon, diacylglycerol kinase eta, diacylglycerol kinase theta, diacylglycerol kinase zeta, diacylglycerol kinase-alpha, diacylglycerol kinase-epsilon, diacylglycerol kinase-zeta, diacylglycerol:ATP kinase, diglyceride kinase, epsilonDGK, eye-specific diacylglycerol kinase, kinase (phosphorylating), 1,2-diacylglycerol, kinase, 1,2-diacylglycerol (phosphorylating), OsDAGK1, Rv2252, sn-1,2-diacylglycerol kinase, type V DGK, YegS, YerQ

ECTree

     2 Transferases

         2.7 Transferring phosphorus-containing groups

             2.7.1 Phosphotransferases with an alcohol group as acceptor

                2.7.1.107 diacylglycerol kinase (ATP)

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Results	in table
126	Activating Compound
35335	AA Sequence
21	Application
1	CAS Registry Number
74	Cloned(Commentary)
53	Cofactor
5	Crystallization (Commentary)
777	Disease/ Diagnostics
9	Expression
70	General Information
11	General Stability
3	IC50 Value
111	Inhibitors
14	Ki Value [mM]
82	KM Value [mM]
162	Localization
79	Metals/Ions
47	Molecular Weight [Da]
91	Natural Substrates/ Products (Substrates)
405	Organism
6	Pathway
106	PDB ID
22	pH Optimum
9	pH Range
2	pI Value
4	Posttranslational Modification
205	Protein Variants
32	Purification (Commentary)
8	Reaction
1	Reaction Type
207	Reference
1	Renatured (Commentary)
308	Source Tissue
44	Specific Activity [micromol/min/mg]
7	Storage Stability
270	Substrates and Products (Substrate)
66	Subunits
201	Synonyms
1	Systematic Name
11	Temperature Optimum [°C]
8	Temperature Stability [°C]
16	Turnover Number [1/s]

Storage Stability

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Storage Stability on EC 2.7.1.107 - diacylglycerol kinase (ATP)

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STORAGE STABILITY

ORGANISM

UNIPROT

LITERATURE

-20°C, in presence of 1 mM dithiothreitol, microsomal and soluble enzyme stable for about 1 week

Rattus norvegicus

-

640368

-80°C, purified isoform DGKepsilon and DGKepsilonDELTA40, 14 weeks, no significant loss of activity

Homo sapiens

P52429

760582

4°C, -20°C or -70°C, more than 50% of the activity is lost after overnight storage in presence of 10 or 20% glycerol

Mesocricetus auratus

Q64398

640381

4°C, enzyme concentrated by polyethyleneglycol 6000, stable for 1 week

Sus scrofa

-

640370

4°C, purified enzyme in 20 mM Tris-HCl, pH 7.4 with 200 mM NaCl, 3 mM CaCl2, 3 mM MgCl2, 0.5 mM dithiothreitol, and 5% (v/v) glycerol, at least 3 months, no loss of activity

Homo sapiens

P23743

762100

4°C, purified enzyme, 50% loss of activity after 3 days

Sus scrofa

-

640370

the 150000 Da enzyme is very unstable but can be stored at -80°C in presence of bovine serum albumin

Sus scrofa

-

640353