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2.7.1.107: diacylglycerol kinase (ATP)

This is an abbreviated version!
For detailed information about diacylglycerol kinase (ATP), go to the full flat file.

Word Map on EC 2.7.1.107

Reaction

ATP
+
1,2-diacyl-sn-glycerol
=
ADP
 +
1,2-diacyl-sn-glycerol 3-phosphate

Synonyms

1,2-diacylglycerol kinase, 1,2-diacylglycerol kinase epsilon, adenosine 5'-triphosphate:1,2-diacylglycerol 3-phosphotransferase, arachidonoyl-specific diacylglycerol kinase, ATP:diacylglycerol phosphotransferase, DAG kinase, DAG-kinase, DAGK, DAGKalpha, DAGKepsilon, DG kinase, DGK, DGK zeta, DGK-1, DGK-3, DGK-3 diacylglycerol kinase, DGK-alpha, DGK-theta, DGK-zeta, DGK1, DGK2, DGK3, DGK4, DGK5, DGK6, DGK7, DGKA, DGKalpha, DgkB, DGKbeta, DGKdelta, DGKE, DGKepsilon, DGKeta, DGKeta1, DGKeta2, DGKeta3, DGKeta4, DGKgamma, DGKiota, DGKkappa, DGKksi, DGKtheta, DGKzeta, diacylglycerol kinase, diacylglycerol kinase (ATP dependent), diacylglycerol kinase 4, diacylglycerol kinase alpha, diacylglycerol kinase beta, diacylglycerol kinase delta, diacylglycerol kinase epsilon, diacylglycerol kinase eta, diacylglycerol kinase theta, diacylglycerol kinase zeta, diacylglycerol kinase-alpha, diacylglycerol kinase-epsilon, diacylglycerol kinase-zeta, diacylglycerol:ATP kinase, diglyceride kinase, epsilonDGK, eye-specific diacylglycerol kinase, kinase (phosphorylating), 1,2-diacylglycerol, kinase, 1,2-diacylglycerol (phosphorylating), OsDAGK1, Rv2252, sn-1,2-diacylglycerol kinase, type V DGK, YegS, YerQ

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.1 Phosphotransferases with an alcohol group as acceptor
                2.7.1.107 diacylglycerol kinase (ATP)

Purification

Purification on EC 2.7.1.107 - diacylglycerol kinase (ATP)

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
a heat-labile 80000 Da enzyme and a heat-stable 150000 Da enzyme
-
apoprotein
-
CHAPS is the most effective detergent for isozyme DGK2 solubilization, NusA/His6-tagged fusion isozyme DGK2 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
DGK I and DGK IV
-
gene dgkA, recombinant N-terminally His-tagged wild-type and mutant enzymes from Escherichia coli strain WH1061 by nickel affinity chromatography and gel filtration. The size-exclusion chromatography step has no effect on specific activity
isoenzyme DGK-I and DGK-II, DGK-II only partially purified
-
native enzyme, by ultracentrifugation, gel filtration, and antibody affinity chromatography
-
Ni-NTA agarose column chromatography and Superdex S200 gel filtration
Ni-NTA agarose column chromatography, Superdex 200 gel filtration or Superdex 75 gel filtration
Ni-NTA agarose column chromatography, Superdex 75 gel filtration and Superdex 200 gel filtration
Ni-NTA column chromatography
Ni-NTA column chromatography and Sephadex G-25M gel filtration
Ni-NTA resin column chromatography
partially, recombinant wild-type and point mutant enzymes from yeast by ion exchange chromatography
-
recombinant FLAG-tagged splicing variants delta1 and delta2 from 3T3-L1 pre-adipocytes by affinity chromatography
-
recombinant GST-fusion isozymes DGKalpha, DGKgamma, and DGKbeta from Escherichia coli, and Sf9 cells, respectively, and from COS-7 cells, by glutathione affinity chromatography
-
recombinant HIs-tagged wild-type and mutant enzymes
-
recombinant human His6-tagged DGK isozyme theta from COS-7 cells by nickel affinity chromatography
recombinant N-terminally His-tagged wild-type and mutant enzymes or MBP-fusion protein from insect cells by nickel affinity chromatography
-
recombinant protein
truncated protein lacking the 40 N-terminal amino acids may be extracted with 1.5 M KCl at neutral pH value, while wild-type protein remains fully membrane bound
-