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2.7.1.1: hexokinase

This is an abbreviated version!
For detailed information about hexokinase, go to the full flat file.

Word Map on EC 2.7.1.1

Reaction

ATP
+
D-glucose
=
ADP
 +
D-glucose 6-phosphate

Synonyms

6-phosphate glucose kinase, AtHXK1, ATP-D-hexose 6-phosphotransferase, ATP-D-hexose-6-phosphotransferase, ATP-dependent hexokinase, ATP: D-glucose 6-phosphotransferase, ATP: D-hexose 6-phosphotransferase, ATP:D-glucose 6-phosphotransferase, ATP:D-hexose 6-phosphotransferase, BmHk, brain form hexokinase, CzHXK1, FgHXK1, FgHXK2, GCK, GK, GKbeta, GlcK, glk, GlkB, glucokinase, glucokinase 1, glucokinase B, glucose ATP phosphotransferase, HaHXK1, hexokinase, hexokinase (phosphorylating), hexokinase 1, hexokinase 2, hexokinase 3, hexokinase 6, hexokinase A, hexokinase D, hexokinase Dor, hexokinase I, hexokinase II, hexokinase III, hexokinase IV, hexokinase PI, hexokinase PII, hexokinase type I, hexokinase type II, hexokinase type IV, hexokinase type IV glucokinase, hexokinase, tumor isozyme, hexokinase-1, hexokinase-10, hexokinase-2, hexokinase-4, hexokinase-5, hexokinase-6, hexokinase-7, hexokinase-8, hexokinase-9, hexokinase-II, hexokinase-like 1, hGK, hGK isoform 1, HK, HK II, HK-1, HK-I, HK-R, HK1, HK2, HK4, HKDC1, HKI, HKII, HKIII, HKL1, HPGLK1, HXK, HXK A, HXK II, Hxk1, HXK10, Hxk2, Hxk3, HXK4, HXK5, HXK6, HXK7, HXK8, HXK9, hxkC, hxkD, kinase, hexo- (phosphorylating), KlHxk1, LGK, LGK2, liver glucokinase, liver glucokinase isoform 2, MODY2 glucokinase, More, muscle form hexokinase, NfGlck, NtHXK1, OsHXK, OsHXK1, OsHXK10, OsHXK2, OsHXK3, OsHXK4, OsHXK5, OsHXK6, OsHXK7, OsHXK9, pHXK, plastid hexokinase, PpHXK1, PpHXK10, PpHXK11, PpHXK2, PpHXK3, PpHXK4, PpHXK5, PpHXK6, PpHXK7, PpHXK8, PpHXK9, Rag5p, ROK hexokinase, ScHK2, ScHXK2, SoHXK1, StHK, STK_23540, StoHK, TbHK1, TbHK2, TcHK, TthHK, type II hexokinase, VIT_18s0001g14230, xprF, Zm.5206, Zm.95484

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.1 Phosphotransferases with an alcohol group as acceptor
                2.7.1.1 hexokinase

Specific Activity

Specific Activity on EC 2.7.1.1 - hexokinase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0001
-
fetal glucokinase
0.0003
-
adult glucokinase
0.008
-
D-fructose as substrate, hxk mutant
0.033 - 0.04
-
-
0.06
-
HK3, at pH 8
0.076
-
D-glucose as substrate, hxk mutant
0.11 - 0.16
-
Hex I and II, at pH 8.2
0.114
-
healthy leaves, soluble glucokinase activity
0.151
-
D-glucose as substrate, wild type hexokinase
0.197
-
D-fructose as substrate, wild type hexokinase
0.199
-
TMV-infected leaves, soluble glucokinase activity
0.25
-
hexokinase type II, at 37°C, pH 8.1
0.26
-
hepatocytes
0.34
-
S151G, D-mannose as substrate
0.55 - 1.95
-
hexokinases Ia, Ib and Ic from adult erythrocytes, at 37°C, pH 7.2
0.57
-
rat hepatocytes treated with adenovirus containing the entire coding sequence of rat liver glucokinase
0.574
-
healthy leaves, particulate glucokinase activity
0.6
-
S151G, D-glucose as substrate
0.609
-
healthy leaves, soluble fructokinase activity
0.688
0.693
-
TMV-infected leaves, particulate glucokinase activity
0.72
-
HK2, at pH 8
0.786
substrate: D-fructose
0.8
-
at 30°C
0.84
-
HK1, at pH 8
0.892
-
TMV-infected leaves, total glucokinase activity
0.941
substrate: D-mannose
1.297
-
healthy leaves, total fructokinase activity
1.4
-
N166R-S151A, D-mannose as substrate
1.43 - 2.22
-
hexokinases Ia, Ib and Ic from umbilical cord blood, at 37°C, pH 7.2
1.44
cell extract of recombinant Escherichia coli expressing the full length enzyme
1.5
-
partially purified liver enzyme
1.95
substrate: D-glucose
110
-
catalytically active 51 kDa C fragment of hexokinase
112
-
pH 8.0, 22°C
122
-
wild-type, D-fructose as substrate
13.06
-
at 30 °C, pH 8.1
137
-
enzyme form Hex A, at 25°C, pH 8
14.5
-
N166R-S151A, D-fructose as substrate
141
purified recombinant catalytic C-terminal domain of the enzyme
145.6
-
erythrocyte, at 37°C
15
-
D-fructose as substrate
150
-
hexokinase from heart, at 25°C, pH 8
165
-
at 37°C
180
-
purified recombinant HK-11aa, a truncated HK I form lacking the first 11 amino acids, at 22°C
19
-
D-mannose as substrate
19.3
pH 7.6, 22°C
19.44
natural hexokinase from epimastigotes, at 25°C, pH 7.5
190
-
pure enzyme, at 25°C, pH 7.6
191.5
-
hexokinase type III, at 37°C, pH 8.1
192.5
-
at 37°C
196
purified recombinant enzyme
2.16
-
S151C, D-mannose as substrate
20.25
-
at pH 7.2
22.2
-
at 25°C, pH 7.2, hexokinase from erythrocytes
23.5
-
hexokinase C, at 30°C, pH 7.5
23.8
-
S151A, D-fructose as substrate
26
pH 7.5, 50°C, substrate: D-glucose
268.8
purified enzyme, substrate D-fructose
27
-
D-glucosamine as substrate
3.18
-
S151G, D-fructose as substrate
3.32
-
S151A, D-mannose as substrate
3.5
cell extract of recombinant Escherichia coli expressing the catalytic C-terminal domain of the enzyme
3.8
-
unpurified recombinant HK-11aa, a truncated HK I form lacking the first 11 amino acids, at 22°C
310
-
N166R, D-fructose as substrate
35.7
-
D-mannose as substrate
36
-
catalytically active recombinant carboxyl-domain of hexokinase type III, at 37°C, pH 8.1
37
-
hexokinase from blood, at 25°C
38.1
-
D-glucose as substrate
4.04
recombinant hexokinase, expressed in Escherichia coli BL21, at 37°C, pH 7
4.1
-
S151A, 2-deoxy-D-glucose as substrate
40
-
D-glucose
45
-
2-deoxy-D-glucose as substrate
5.6
-
in purified glycosomes, at 25°C, pH 7.6
58
-
hexokinase from heart, at 25°C, pH 7.2
58.2
-
recombinant hexokinase I expressed in E. coli
6.6
-
S151A, D-glucose as substrate
60 - 61
wild-type and nonaggregating interface mutant hexokinase I, pH 7.8
60.8
-
N166R, D-mannose as substrate
70
-
purified liver enzyme
8.4
-
at 10 mM D-glucose
80
-
wild-type and N166R, D-glucose as substrate
86
-
hexokinase B, at 30°C, pH 7.5
9.12
-
S151C, D-fructose as substrate
9.27
recombinant hexokinase expressed in Escherichia coli XL-1 Blue, at 25°C, pH 7.5
94
purified recombinant full length enzyme
95.7
purified enzyme, substrate D-glucose
98.7
-
D-fructose as substrate
additional information