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2.7.1.1: hexokinase

This is an abbreviated version!
For detailed information about hexokinase, go to the full flat file.

Word Map on EC 2.7.1.1

Reaction

ATP
+
D-glucose
=
ADP
 +
D-glucose 6-phosphate

Synonyms

6-phosphate glucose kinase, AtHXK1, ATP-D-hexose 6-phosphotransferase, ATP-D-hexose-6-phosphotransferase, ATP-dependent hexokinase, ATP: D-glucose 6-phosphotransferase, ATP: D-hexose 6-phosphotransferase, ATP:D-glucose 6-phosphotransferase, ATP:D-hexose 6-phosphotransferase, BmHk, brain form hexokinase, CzHXK1, FgHXK1, FgHXK2, GCK, GK, GKbeta, GlcK, glk, GlkB, glucokinase, glucokinase 1, glucokinase B, glucose ATP phosphotransferase, HaHXK1, hexokinase, hexokinase (phosphorylating), hexokinase 1, hexokinase 2, hexokinase 3, hexokinase 6, hexokinase A, hexokinase D, hexokinase Dor, hexokinase I, hexokinase II, hexokinase III, hexokinase IV, hexokinase PI, hexokinase PII, hexokinase type I, hexokinase type II, hexokinase type IV, hexokinase type IV glucokinase, hexokinase, tumor isozyme, hexokinase-1, hexokinase-10, hexokinase-2, hexokinase-4, hexokinase-5, hexokinase-6, hexokinase-7, hexokinase-8, hexokinase-9, hexokinase-II, hexokinase-like 1, hGK, hGK isoform 1, HK, HK II, HK-1, HK-I, HK-R, HK1, HK2, HK4, HKDC1, HKI, HKII, HKIII, HKL1, HPGLK1, HXK, HXK A, HXK II, Hxk1, HXK10, Hxk2, Hxk3, HXK4, HXK5, HXK6, HXK7, HXK8, HXK9, hxkC, hxkD, kinase, hexo- (phosphorylating), KlHxk1, LGK, LGK2, liver glucokinase, liver glucokinase isoform 2, MODY2 glucokinase, More, muscle form hexokinase, NfGlck, NtHXK1, OsHXK, OsHXK1, OsHXK10, OsHXK2, OsHXK3, OsHXK4, OsHXK5, OsHXK6, OsHXK7, OsHXK9, pHXK, plastid hexokinase, PpHXK1, PpHXK10, PpHXK11, PpHXK2, PpHXK3, PpHXK4, PpHXK5, PpHXK6, PpHXK7, PpHXK8, PpHXK9, Rag5p, ROK hexokinase, ScHK2, ScHXK2, SoHXK1, StHK, STK_23540, StoHK, TbHK1, TbHK2, TcHK, TthHK, type II hexokinase, VIT_18s0001g14230, xprF, Zm.5206, Zm.95484

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.1 Phosphotransferases with an alcohol group as acceptor
                2.7.1.1 hexokinase

Purification

Purification on EC 2.7.1.1 - hexokinase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
106fold by 2 steps of ion exchange chromatography, and gel filtration
120fold: recombinant carboxyl-domain of hexokinase type III, partial: hexokinase type III from lymphocytes
-
13fold
-
146fold
-
253fold, major isoenzyme form
-
3 isoenzymes PI, PII and glucokinase, partial
-
3 isoenzymes: HK1: 140fold, HK2: 40fold, HK3: 25fold
-
48.6fold: natural hexokinase from epimastigotes, 66.3fold: recombinant hexokinase expressed in Escherichia coli XL-1 Blue
about 600fold
-
affinity chromatography
-
erythrocyte enzyme and reticulocyte hexokinase Ia and Ib
-
erythrocyte enzyme: 300000fold
-
erythrocyte hexokinase type I, 84000fold
-
from blood: 175000fold, from heart: 1700fold
-
from cytosol, hexokinase B: 539fold, hexokinase C: 235fold
-
from erythrocytes: 110000fold, and from heart
-
from liver, to homogeneity
-
from muscle
-
from overproducing strain
-
full length recombinant protein
glutathione S-transferase (GST)-GlkB fusion proteins, wild-type and mutant
glutathione Sepharose column chromatography, Sephadex G-25 gel filtration
hexokinase and glucokinase
-
hexokinase I from brain
hexokinase I, 103fold
-
hexokinase Ia: 55460fold, hexokinase Ib: 51260fold
-
hexokinase III: 290000fold, hexokinase II: partial, 39160fold
-
hexokinase type II, 5330fold
-
HisTrap column chromatography and Superose 6 gel filtration
-
intact hexokinase I, isolation of a catalytically active 51 kDa C fragment and a 52 kDa N fragment without catalytic activity
-
isoenzymes Hex I and Hex II, partial
-
isoenzymes PI and PII
-
isoenzymes PI, PII and PIIM
-
isozymes HKI and HKII, 170fold, from immature seeds, by ammonium sulfate fractionation, ion exchange chromatography, and gel filtration
-
kinetic-based biospecific affinity chromatographic studies
-
Ni-NTA column chromatography
-
Ni-NTA column chromatography, Hitrap Q FF column chromatography, and Superdex 200 gel filtration
-
Ni-NTA resin column chromatography
nickel and FLAG affinity column chromatography and gel filtration
-
partial
partial, 52240fold
-
partial, from adult and fetal erythrocytes, 1000-2000fold
-
partially, subcellular fractionation by anion exchange chromatography of isozymes from healthy and TMV-infected leaves
-
phenyl-Sepharose column chromatography, ammonium sulfate precipitation, Q-Sepharose column chromatography, glutathione-Sepharose 4B column chromatography
purification of 2 enzyme forms: Hex A: 1370fold, Hex C2: 600fold
-
recombinant
-
recombinant enzyme
recombinant enzyme from enzyme-deficient strain JA6DELTArag5, 25fold by anion exchange chromatography and ammonium sulfate fractionation
recombinant enzyme from Escherichia coli strain MV1184, to homogeneity by heat precipitation at 80°C, successive ion exchange chromatography and gel filtration
recombinant FLAG-tagged hepatic wild-type and mutant enzymes from Escherichia coli strain DH5 alpha by ion exchange and glucosamine affinity chromatography, and gel filtration
recombinant full length enzyme and C-terminal domain from Escherichia coli strain BL21(DE3) by ion exchange, hydrophobic interaction, anddye ligand affinity chromatography, and gel filtration
recombinant full-length HK I, truncate form lacking the first 11 amino acids named HK-11aa, 50 kDa C-terminal half containing the catalytic domain
-
recombinant hexokinase I expressed in Escherichia coli BL21(DE3)
-
recombinant hexokinase, expressed in Escherichia coli
recombinant hexokinase, expressed in Escherichia coli BL21
recombinant hexokinases II and III, expressed in yeast
-
recombinant HXK1 and HXK2, expressed in Escherichia coli K38
-
recombinant N-terminally His6-tagged hepatic enzyme from Escherichia coli by nickel affinity chromatography to over 90% purity
-
recombinant N-terminally His6-tagged pancreatic enzyme from Escherichia coli by nickel affinity chromatography to over 90% purity
-
recombinant wild-type and mutant enzymes from Escherichia coli to homogeneity
-
recombinant wild-type and nondimerizing mutant hexokinase I, expressed in Escherichia coli ZSC13
solubilization studies of mitochondrial HK I
-
soluble hexokinase type I with 2 unseparated subtypes, 11600fold
-
sperm membrane hexokinase p95/116
-
using affinity and ion-exchange chromatography
-
using hydrophobic-interaction chromatography on a butyl-sepharose™ 4 Fast Flow column. Main hexokinase isoform is purified to homogeneity using further chromatographic separations on red dye, DEAE Fractogel, hydroxyapatite, cibacron blue, and MonoQ matrices
-
using immobilized metal ion affinity chromatography
using Ni-NTA chromatography
using Ni-NTA chromatography followed by ion-exchange chromatography on a HiTrap Q FF column and gel filtration on a Superdex 200 column
wild type, mutant V62M, and E300K GST-glucokinase proteins
-