2.6.1.98: UDP-2-acetamido-2-deoxy-ribo-hexuluronate aminotransferase
This is an abbreviated version!
For detailed information about UDP-2-acetamido-2-deoxy-ribo-hexuluronate aminotransferase, go to the full flat file.
Reaction
Synonyms
aminotransferase WbpE, PGN_1236, PorR, WbpE, WlbC, WpbE
ECTree
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Crystallization
Crystallization on EC 2.6.1.98 - UDP-2-acetamido-2-deoxy-ribo-hexuluronate aminotransferase
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purifed recombinant detagged WbpE in complex with the cofactor pyridoxal 5'-phosphate and product UDP-2-acetamido-3-amino-2,3-dideoxy-D-glucuronic acid as the external aldimine, mixing of 0.0015 ml of protein solution containing 10 mg/ml protein in 25 mM HEPES, pH 8.0, 100 mM NaCl, 0.5% glycerol, and 0.5 mM UDP-2-acetamido-3-amino-2,3-dideoxy-D-glucuronic acid, with 0.0015 ml of reservoir solution containing 0.1 M Bis-Tris, pH 5.5, 0.2 M ammonium sulfate and 25% PEG 3350 for the wild-type enzyme, and 0.1 M Bis-Tris, pH 5.5, 0.2 M ammonium acetate, 10 mM SrCl2 and 25% PEG 3350 for the sselenomethionine-labeled enzyme, X-ray diffraction structure determination and analysis at 1.9 A resolution, molecular replacement
purified enzyme in complex with dTDP-3-amino-3,6-dideoxy-D-galactose and dTDP-3-amino-3,6-dideoxy-D-glucose, X-ray diffraction structure determination and analysis at 1.3 A resolution, structure correction