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2.6.1.96: 4-aminobutyrate-pyruvate transaminase

This is an abbreviated version!
For detailed information about 4-aminobutyrate-pyruvate transaminase, go to the full flat file.

Word Map on EC 2.6.1.96

Reaction

4-aminobutanoate
+
glyoxylate
=
succinate semialdehyde
 +
glycine

Synonyms

4-aminobutyrate transaminase, BnaA.GABA-T.a, BnaA.GABA-T.b, BnaA01g24820D, BnaA05g17430D, BnaC.GABA-T.a, BnaC.GABA-T.b, BnaC05g30410D, GABA transaminase, GABA-T, GABA-T2, GABA-T3, gaba:pyruvate transaminase, GABA:pyruvate-T, gamma-aminobutyrate transaminase, gamma-aminobutyrate transaminase 1, mitochondrial, gamma-aminobutyric acid pyruvate transaminase, gamma-aminobutyric acid transaminase, gammaaAminobutyric acid transaminase, Os04g0614500, Osl2, Pop2, pyruvate-specific mitochondrial GABA-T

ECTree

     2 Transferases
         2.6 Transferring nitrogenous groups
             2.6.1 Transaminases
                2.6.1.96 4-aminobutyrate-pyruvate transaminase

Cloned

Cloned on EC 2.6.1.96 - 4-aminobutyrate-pyruvate transaminase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis, functional expression in Escherichia coli
gene GABA-T1, sequencce comparisons, expression of isozyme GABA-T1 in Escherichia coli strain BL21 (DE3), transient expression of fluorescence-labeled GABA-T proteins in onion epidermal cells, two of the four enzymes are targeted to mitochondria, a third to chloroplasts, and the fourth to cytosol
gene GABA-T2, sequencce comparisons, expression of isozyme GABA-T2 in Escherichia coli strain BL21 (DE3), transient expression of fluorescence-labeled GABA-T proteins in onion epidermal cells, two of the four enzymes are targeted to mitochondria, a third to chloroplasts, and the fourth to cytosol
gene GABA-T3, sequence comparisons, expression of isozyme GABA-T3 in Escherichia coli strain BL21 (DE3), transient expression of fluorescence-labeled GABA-T proteins in onion epidermal cells, two of the four enzymes are targeted to mitochondria, a third to chloroplasts, and the fourth to cytosol
gene GABA-T4, sequencce comparisons, expression of isozyme GABA-T4 in Escherichia coli strain BL21 (DE3), transient expression of fluorescence-labeled GABA-T proteins in onion epidermal cells, two of the four enzymes are targeted to mitochondria, a third to chloroplasts, and the fourth to cytosol
gene pop2, expression of N-termminally His6-tagged GABA-T POP2 in Escherichia coli strain BL-21(DE3) Rosetta (pLysS)
genes pop2-1, pop2-2, and pop2-3, pop2-1 are located on chromosome 3, DNA and amino acid sequennce determination and analysis
isozyme GABA-T1, DNA and amino acid sequence determination and analysis, expression of His-tagged GABA-T1 in Escherichia coli strain BL-21(DE3) Rosetta (pLysS), expression and subcellular localization of tomato GABA-T isozymes in Nicotiana tabacum BY-2 suspension-cultured cells
isozyme GABA-T2, DNA and amino acid sequence determination and analysis, expression of His-tagged GABA-T2 in Escherichia coli strain BL-21(DE3) Rosetta (pLysS), expression and subcellular localization of tomato GABA-T isozymes in Nicotiana tabacum BY-2 suspension-cultured cells
isozyme GABA-T3, DNA and amino acid sequence determination and analysis, expression of His-tagged GABA-T3 in Escherichia coli strain BL-21(DE3) Rosetta (pLysS), expression and subcellular localization of tomato GABA-T isozymes in Nicotiana tabacum BY-2 suspension-cultured cells
removal of the N-terminal targeting presequences yields good recovery of the soluble recombinant proteins in Escherichia coli when they are co-expressed with the GroES/EL molecular chaperone complex
transient expression of GABA-T in Nicotiana tabacum BY-2 cells. C-terminal myc-tagged GABA-T co-localizes with coexpressed mitochondrial betaATPase-CAT in BY-2 cells