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2.6.1.7: kynurenine-oxoglutarate transaminase

This is an abbreviated version!
For detailed information about kynurenine-oxoglutarate transaminase, go to the full flat file.

Word Map on EC 2.6.1.7

Reaction

L-kynurenine
+
2-oxoglutarate
=
4-(2-aminophenyl)-2,4-dioxobutanoate
 +
L-glutamate

Synonyms

AadAT, aminoadipate aminotransferase, aminotransferase, kynurenine, aspartate aminotransferase, Bna3, CCBL2, cysteine conjugate beta-lyase 1, cysteine conjugate beta-lyase 2, glutamic-oxaloacetic transaminase 2, glutamine transaminase K, KAT, KAT I, KAT II, KAT III, KAT IV, KAT-1, KAT-I, KAT-II, KAT2, KAT3, KAT3/GTL/CCBL2, KYAT3, kynurenine 2-oxoglutarate transaminase, kynurenine aminotransferase, kynurenine aminotransferase 2, kynurenine aminotransferase 3, kynurenine aminotransferase I, kynurenine aminotransferase II, kynurenine aminotransferase III, kynurenine aminotransferase IV, kynurenine aminotransferase-1, kynurenine aminotransferase-I, kynurenine aminotransferase-IV, kynurenine aminotransferases I, kynurenine aminotransferases II, kynurenine pyruvate aminotransferase, kynurenine transaminase (cyclizing), kynurenine-2-oxoglutarate aminotransferase, kynurenine/alpha-aminoadipate aminotransferase, L-kynurenine aminotransferase, mitochondrial aspartate aminotransferase, More, PhKAT, phKAT-II, type II kynurenine aminotransferase

ECTree

     2 Transferases
         2.6 Transferring nitrogenous groups
             2.6.1 Transaminases
                2.6.1.7 kynurenine-oxoglutarate transaminase

Purification

Purification on EC 2.6.1.7 - kynurenine-oxoglutarate transaminase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
acid precipitation, ammonium sulfate, DEAE-cellulose, hydroxylapatite
-
affinity chromatography, ion exchange chromatography (Q-Sepharose), gel filtration
ammonium sulfate precipitation, phenyl Sepharose column chromatography, DEAE Sepharose column chromatography, Blue Sepharose column chromatography, and Superdex 200 gel filtration
ammonium sulfate, DEAE-Sepharose, phenyl-Sepharose, Mono Q, Mono P
chitin affinity chromatography, cleavage of tag domain, ion exchange chromatography (DEAE-Sepharose and Mono-Q), gel filtration
DEAE Sepharose column chromatography, hydroxyapatite column chromatography, Mono-Q column chromatography, and gel filtration
DEAE-cellulose, heat treatment, hydroxyapatite, CM-Sephadex C-25, Sephadex G-100
-
DEAE-Sepharose column chromatography, hydroxyapatite column chromatography, and gel filtration
DEAE-Sepharose, ammonium sulfate, Sephadex G-25, partial purification of KAT I and KAT II
-
DEAE-Sepharose, phenyl-Sepharose, Superose 12, Mono Q
-
glutathione-Sepharose column chromatography
immobilized metal ion affinity chromatography (Ni2+)
partial purification
-
partial purification of kynurenine apotransaminase
-
polyethyleneimine, DEAE-cellulose, Bio-Gel P-300, hydroxyapatite, crystallization
Hansenula schneggii
-
recombinant His-tagged enzyme from Escherichia coli by nickel affinity purification, two different steps of anion exchange chromatography, and gel filtration
-
recombinant protein
truncated recombinant enzyme, DEAE-Sepharose, native PAGE
Q95VY4