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0.59
2-norpyridoxal
-
pH 8.9, 25°C
0.16
2-Norpyridoxamine
-
pH 8.9, 25°C
8.8 - 110.3
2-oxoglutarate
0.89
3-hydroxypyridine-4-aldehyde
-
pH 8.9, 25°C
0.009
5-Deoxypyridoxal
-
pH 8.9, 25°C
0.014
5-Deoxypyridoxamine
-
pH 8.9, 25°C
1.3
omega-methylpyridoxal
-
pH 8.9, 25°C
3.4
pyridoxamine 5'-phosphate
mutant E68A, pH 9.0, 30°C
additional information
additional information
-
20
(S)-2-aminobutyrate
-
20
(S)-2-aminobutyrate
30°C, pH 9.0
7.1
2-oxobutyrate
-
7.1
2-oxobutyrate
30°C, pH 9.0
8.8
2-oxoglutarate
Y35H/V70R/F247C mutant protein, pH 9.0, 30°C
12.6
2-oxoglutarate
Y35H/V70K mutant protein, pH 9.0, 30°C
17.2
2-oxoglutarate
M2T/Y35H/V70K/E212G mutant protein, pH 9.0, 30°C
21.1
2-oxoglutarate
M2T/Y35H/V70K mutant protein, pH 9.0, 30°C
61.3
2-oxoglutarate
V70R/F247C mutant protein, pH 9.0, 30°C
77.3
2-oxoglutarate
M2T/V70K mutant protein, pH 9.0, 30°C
80.1
2-oxoglutarate
wild type protein, pH 9.0, 30°C
102.6
2-oxoglutarate
V70K mutant protein, pH 9.0, 30°C
106.8
2-oxoglutarate
M2T/V70K/E212G mutant protein, pH 9.0, 30°C
110.3
2-oxoglutarate
V70K/E212G mutant protein, pH 9.0, 30°C
0.58
L-alanine
-
with omega-methylpyridoxal, pH 8.9, 25°C
1.6
L-alanine
-
with pyridoxal, pH 8.9, 25°C
1.9
L-alanine
-
with 5-deoxypyridoxal, pH 8.9, 25°C
2.7
L-alanine
-
with 2-norpyridoxal, pH 8.9, 25°C
11
L-alanine
30°C, pH 9.0
64.5
L-alanine
-
with 3-hydroxypyridine-4-aldehyde, pH 8.9, 25°C
29.3
L-glutamate
Y35H/V70K mutant protein, pH 9.0, 30°C
42.4
L-glutamate
Y35H/V70R/F247C mutant protein, pH 9.0, 30°C
44.2
L-glutamate
M2T/Y35H/V70K mutant protein, pH 9.0, 30°C
51
L-glutamate
M2T/Y35H/V70K/E212G mutant protein, pH 9.0, 30°C
123.4
L-glutamate
wild type protein, pH 9.0, 30°C
133.5
L-glutamate
V70R/F247C mutant protein, pH 9.0, 30°C
142
L-glutamate
V70K mutant protein, pH 9.0, 30°C
0.012
pyridoxal
-
pH 8.9, 25°C
0.059
pyridoxal
30°C, pH 9.0
0.013
pyridoxamine
-
pH 8.9, 25°C
0.043
pyridoxamine
-
pH 8.5, 37°C
0.044
pyridoxamine
30°C, pH 9.0
0.044
pyridoxamine
wild-type, pH 9.0, 30°C
0.09
pyridoxamine
-
pH 8.5, 37°C
0.1
pyridoxamine
mutant R336A, pH 9.0, 30°C
0.35
pyridoxamine
-
with 3-hydroxy-4-aminomethylpyridine, pH 8.9, 25°C
1.3
pyridoxamine
mutant E68A, pH 9.0, 30°C
2.5
pyridoxamine
mutant E68G, pH 9.0, 30°C
0.1
pyruvate
-
pH 8.5, 37°C
0.31
pyruvate
-
pH 8.5, 37°C
0.34
pyruvate
30°C, pH 9.0
0.34
pyruvate
wild-type, pH 9.0, 30°C
0.35
pyruvate
-
with 3-hydroxy-4-aminomethylpyridine, pH 8.9, 25°C
0.4
pyruvate
-
with omega-methylpyridoxamine, pH 8.9, 25°C
0.42
pyruvate
-
with 5-deoxypyridoxamine, pH 8.9, 25°C
0.52
pyruvate
-
with 2-norpyridoxamine, pH 8.9, 25°C
6.9
pyruvate
mutant R336A, pH 9.0, 30°C
7.4
pyruvate
-
with 3-hydroxy-4-aminomethylpyridine, pH 8.9, 25°C
additional information
additional information
-
kinetics
-
additional information
additional information
-
kinetics
-
additional information
additional information
-
kinetics
-
additional information
additional information
Soil bacterium
-
effect of pH on kinetic parameters
-
2.6.1.30 pyridoxamine-pyruvate transaminase
results (
results (
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