2.6.1.30: pyridoxamine-pyruvate transaminase
This is an abbreviated version!
For detailed information about pyridoxamine-pyruvate transaminase, go to the full flat file.
Word Map on EC 2.6.1.30
-
2.6.1.30
-
pyridoxal
-
mesorhizobium
-
loti
-
5'-phosphate
-
transamination
-
5'-deoxypyridoxal
-
l-alanine
-
pyridoxine
-
nabh4
-
4-oxidase
-
analysis
-
synthesis
- 2.6.1.30
- pyridoxal
-
mesorhizobium
- loti
- 5'-phosphate
-
transamination
- 5'-deoxypyridoxal
- l-alanine
- pyridoxine
- nabh4
-
4-oxidase
- analysis
- synthesis
Reaction
Synonyms
aminotransferase, pyridoxamine-pyruvate, PM-pyruvate transaminase, PPAT, pyridoxamine-pyruvate aminotransferase, pyridoxamine-pyruvic transaminase
ECTree
2.6.1.30 pyridoxamine-pyruvate transaminaseAdvanced search results
results (
results (Engineering
Engineering on EC 2.6.1.30 - pyridoxamine-pyruvate transaminase
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
top
PROTEIN VARIANTS 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
C198A
E68A
low pyridoxamine 5'-phosphate:pyruvate aminotransferase activity, decrease in activity towards pyridoxamine
E68G
low pyridoxamine 5'-phosphate:pyruvate aminotransferase activity, decrease in activity towards pyridoxamine
K197L
V70K
active site residue, increase in reactivity toward 2-oxoglutarate
E68A
-
low pyridoxamine 5'-phosphate:pyruvate aminotransferase activity, decrease in activity towards pyridoxamine
-
E68G
-
low pyridoxamine 5'-phosphate:pyruvate aminotransferase activity, decrease in activity towards pyridoxamine
-
additional information
C198A
enzyme shows the same specific activity as that of the wild type enzyme
additional information
pyridoxamine is produced from pyridoxine by bioconversion using a Rhodococcus expression system. Approximately 450 mM pyridoxal are produced from 500 mM pyridoxine using recombinant Rhodococcus erythropolis expressing the pyridoxine 4-oxidase gene derived from Mesorhizobium loti. In the bioconversion of pyridoxal to pyridoxamine using recombinant Rhodococcus erythropolis expressing the pyridoxamine-pyruvate aminotransferase gene derived from Mesorhizobium loti, the bioconversion rate is approximately 80% under the same conditions as pyridoxal production. Finally, in the bioconversion of pyridoxine to pyridoxamine through pyridoxal using recombinant Rhodococcus erythropolis coexpressing the genes for pyridoxine 4-oxidase and pyridoxamine-pyruvate aminotransferase, the bioconversion rate is approximately 75%. Reactor bioconversion using the Rhodococcus expression system, method evaluation and optimization, overview
additional information
-
pyridoxamine is produced from pyridoxine by bioconversion using a Rhodococcus expression system. Approximately 450 mM pyridoxal are produced from 500 mM pyridoxine using recombinant Rhodococcus erythropolis expressing the pyridoxine 4-oxidase gene derived from Mesorhizobium loti. In the bioconversion of pyridoxal to pyridoxamine using recombinant Rhodococcus erythropolis expressing the pyridoxamine-pyruvate aminotransferase gene derived from Mesorhizobium loti, the bioconversion rate is approximately 80% under the same conditions as pyridoxal production. Finally, in the bioconversion of pyridoxine to pyridoxamine through pyridoxal using recombinant Rhodococcus erythropolis coexpressing the genes for pyridoxine 4-oxidase and pyridoxamine-pyruvate aminotransferase, the bioconversion rate is approximately 75%. Reactor bioconversion using the Rhodococcus expression system, method evaluation and optimization, overview
-
additional information
-
pyridoxamine is produced from pyridoxine by bioconversion using a Rhodococcus expression system. Approximately 450 mM pyridoxal are produced from 500 mM pyridoxine using recombinant Rhodococcus erythropolis expressing the pyridoxine 4-oxidase gene derived from Mesorhizobium loti. In the bioconversion of pyridoxal to pyridoxamine using recombinant Rhodococcus erythropolis expressing the pyridoxamine-pyruvate aminotransferase gene derived from Mesorhizobium loti, the bioconversion rate is approximately 80% under the same conditions as pyridoxal production. Finally, in the bioconversion of pyridoxine to pyridoxamine through pyridoxal using recombinant Rhodococcus erythropolis coexpressing the genes for pyridoxine 4-oxidase and pyridoxamine-pyruvate aminotransferase, the bioconversion rate is approximately 75%. Reactor bioconversion using the Rhodococcus expression system, method evaluation and optimization, overview
-
