2.6.1.30: pyridoxamine-pyruvate transaminase

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For detailed information about pyridoxamine-pyruvate transaminase, go to the full flat file.

Reaction

Synonyms

aminotransferase, pyridoxamine-pyruvate, PM-pyruvate transaminase, PPAT, pyridoxamine-pyruvate aminotransferase, pyridoxamine-pyruvic transaminase

ECTree

     2 Transferases

         2.6 Transferring nitrogenous groups

             2.6.1 Transaminases

                2.6.1.30 pyridoxamine-pyruvate transaminase

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Results	in table
1	Activating Compound
26	AA Sequence
4	Application
1	CAS Registry Number
5	Cloned(Commentary)
4	Cofactor
2	Crystallization (Commentary)
1	General Stability
8	Inhibitors
17	kcat/KM [mM/s]
63	KM Value [mM]
1	Metals/Ions
7	Molecular Weight [Da]
9	Natural Substrates/ Products (Substrates)
26	Organism
3	Pathway
8	PDB ID
4	pH Optimum
2	pH Range
25	Protein Variants
5	Purification (Commentary)
4	Reaction
1	Reaction Type
16	Reference
1	Source Tissue
2	Specific Activity [micromol/min/mg]
1	Storage Stability
37	Substrates and Products (Substrate)
11	Subunits
21	Synonyms
1	Systematic Name
7	Temperature Optimum [°C]
2	Temperature Range [°C]
2	Temperature Stability [°C]
36	Turnover Number [1/s]

Crystallization

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Crystallization on EC 2.6.1.30 - pyridoxamine-pyruvate transaminase

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CRYSTALLIZATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

native enzyme, space group P43212, diffraction to 2.0 A resolution. Complexes with pyridoxamine, pyridoxal, and pyridoxyl-L-alanine at 1.7 A, 1.7 A, and 2.0 A resolution, respectively. Enzyme is a homotetramer and each subunit is composed of a large N-terminal domain, consisting of seven beta-sheets and eight alpha-helices, and a smaller C-terminal domain, consisting of three beta-sheets and four alpha-helices. The substrate pyridoxal is bound through an aldimine linkage to Lys197 in the active site. The carboxylate group of the substrate amino/keto acid is hydrogen-bonded to Arg336 and Arg345

Mesorhizobium loti

Q988B8

687694

addition of saturated ammonium sulfate solution to the concentrated protein solution until the first permanent turbidity appears, several days at 5°C

Pseudomonas sp.

-

639956