2.6.1.21: D-amino-acid transaminase
This is an abbreviated version!
For detailed information about D-amino-acid transaminase, go to the full flat file.
Word Map on EC 2.6.1.21
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2.6.1.21
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pyridoxal
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racemase
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transamination
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d-glutamate
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pyridoxamine
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sphaericus
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quinonoid
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aldimine
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4-amino-4-deoxychorismate
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yoshimura
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ketimine
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pyridoxal-5'-phosphate-dependent
- 2.6.1.21
- pyridoxal
- racemase
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transamination
- d-glutamate
- pyridoxamine
- sphaericus
-
quinonoid
-
aldimine
- 4-amino-4-deoxychorismate
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yoshimura
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ketimine
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pyridoxal-5'-phosphate-dependent
Reaction
Synonyms
aminotransferase, D-alanine, AspFum, AspOry, AspTer, AtDAT1, D-AA transaminase, D-AAT, D-alanine aminotransferase, D-alanine transaminase, D-alanine:2-oxoglutarate aminotransferase, D-amino acid aminotransferase, D-amino acid transaminase, D-aspartate transaminase, D-aspartic aminotransferase, DAA aminotransferase, DAA transaminase, DAAT, DAT, DatA, Dret, Dret_1107, EC 2.6.1.10, FQP89_01185, GibZea, MSMEG_5795, MycVan, NeoFis, PenChr, TRA-01185
ECTree
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Cloned
Cloned on EC 2.6.1.21 - D-amino-acid transaminase
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expressed in Escherichia coli
expression of wild-type, L201A and L201W mutant enzymes in Escherichia coli
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gene DAAT, construction of expression plasmid pENTR-AtDAT1(Col-0), pENTR-AtDAT1(A77T), pENTR-AtDAT1(T303S), and pENTR-AtDAT1(Ler), recombinant expression of C-terminally GST and N-terminally His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) RIL. The wild-type and mutant enzymes are transfected into Arabdiospsis thaliana and Nicotianan benthamiana plants via the Agrobacterium tumefaciens strain pMP90-RK GV3101 transfection system, leaf infiltration resulting in insertion lines, SALK_011686 and SALK_111981, denoted as dat1-1 and dat1-2, respectively. Histochemical staining of GUS activity in seedlings and adult plants incubated overnight at 37°C in this buffer containing 1 mM X-Gluc (5-bromo-4-chloro-3-indolyl-beta-D-glucuronic acid) and 0.5 mM K3Fe(CN)6. RT-PCR enzyme expression analysis
gene Dret_1107, construction of the synthetic gene coding the transaminase Dret from Desulfohalobium retbaense, recombinant expression
gene FQP89_01185, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, quantitative RT-PCR enzyme expression analysis, recombinant expression in Escherichia coli strain BL21(DE3)
recombinant expression of C-terminally His-tagged wild-type DATA enzyme and mutant enzymes in Escherichia coli strain BL21(DE3)