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a recombinant seedling OAT is obtained by cDNA expression in Escherichia coli and its substrate specificity is measured, the enzyme is found to be strictly specific for L-ornithine showing practically no activity with putrescine, 1,3-diamimopropane and 4-aminobutyrate
calmodulin-binding peptide fusion protein
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expressed in Escherichia coli
expressed in Escherichia coli as a His-tagged fusion protein
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expression in Escherichia coli
expression of the entire human gene in Escherichia coli
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fusion with maltose binding protein
gene TaOAT-5AL, located on chromosome 5, DNA and amino acid sequence determination and analysis, sequence alignment between genomic DNA and its corresponding cDNA obtains a total of ten exons and nine introns, the three genes have 87.68 and 87.18% identity at the gDNA and cDNA levels, respectively, genetic structure, and phylogenetic tree. Two transcript variants of TaOAT-5AL are revealed, named TaOAT-5AL-1 and TaOAT-5AL-2 and characterized by 1497 bp and 1287 bp in cDNA length, respectively. Compared to TaOAT-5AL-2, TaOAT-5AL-1 contains an additional 120-bp insertion encompassing an in-frame stop codon, which resulted in a premature protein. The additional insertion is genotypically confirmed by sequencing results from six cultivars. The TaOAT-5AL-1 gene transcript variant contains in-frame stop codon that causes the incomplete translation of protein. Recombinant overexpression of TaOAT-AL-2 in Triticum aestivum plants, recombinant expression of C-terminally GFP-tagged enzyme in Triticum aestivum plants in mitochondria, quantitative reverse transcription PCR (qRT-PCR) analysis
gene TaOAT-5BL, located on chromosome 5, DNA and amino acid sequence determination and analysis, sequence alignment between genomic DNA and its corresponding cDNA obtains a total of ten exons and nine introns, the three genes have 87.68 and 87.18% identity at the gDNA and cDNA levels, respectively, genetic structure and phylogenetic tree, recombinant overexpression in Triticum aestivum plants, recombinant expression of C-terminally GFP-tagged enzyme in triticum aestivum plants in mitochondria quantitative reverse transcription PCR (qRT-PCR) analysis
gene TaOAT-5DL, located on chromosome 5, DNA and amino acid sequence determination and analysis, sequence alignment between genomic DNA and its corresponding cDNA obtains a total of ten exons and nine introns, the three genes have 87.68 and 87.18% identity at the gDNA and cDNA levels, respectively, genetic structure and phylogenetic tree, recombinant overexpression in Triticum aestivum plants, recombinant expression of C-terminally GFP-tagged enzyme in triticum aestivum plants in mitochondria, quantitative reverse transcription PCR (qRT-PCR) analysis
it is believed that there is only one gene for OAT, pseudo-genes are not transcribed or lead to non-functional proteins, DNA and amino acid sequence comparisons and phylogenetic analysis
it is believed that there is only one gene for OAT, pseudo-genes are not transcribed or lead to non-functional proteins, DNA and amino acid sequence comparisons and phylogenetic analysis, genetic organization and localization of OAT genes, overview
mothbean enzyme is expressed in Escherichia coli
Nicotiana plumbaginifolia plants overexpressing OAT from Arabidopsis synthesize more proline than the control plants and show a higher biomass and a higher germination rate under osmotic stress conditions
recombinant enzyme expression in Escherichia coli strain BL21(DE3)pLysS
recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) Codon Plus
recombinant expression of mutant R180T enzyme in CHO-K1 cells
recombinant expression of wild-type and mutant enzymes in Corynebacterium glutamincum strain 1006, subcloning in Escherichia coli strain DH5alpha
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expression in Escherichia coli
expression in Escherichia coli
it is believed that there is only one gene for OAT, pseudo-genes are not transcribed or lead to non-functional proteins, DNA and amino acid sequence comparisons and phylogenetic analysis
it is believed that there is only one gene for OAT, pseudo-genes are not transcribed or lead to non-functional proteins, DNA and amino acid sequence comparisons and phylogenetic analysis
it is believed that there is only one gene for OAT, pseudo-genes are not transcribed or lead to non-functional proteins, DNA and amino acid sequence comparisons and phylogenetic analysis
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it is believed that there is only one gene for OAT, pseudo-genes are not transcribed or lead to non-functional proteins, DNA and amino acid sequence comparisons and phylogenetic analysis
it is believed that there is only one gene for OAT, pseudo-genes are not transcribed or lead to non-functional proteins, DNA and amino acid sequence comparisons and phylogenetic analysis
it is believed that there is only one gene for OAT, pseudo-genes are not transcribed or lead to non-functional proteins, DNA and amino acid sequence comparisons and phylogenetic analysis
it is believed that there is only one gene for OAT, pseudo-genes are not transcribed or lead to non-functional proteins, DNA and amino acid sequence comparisons and phylogenetic analysis
it is believed that there is only one gene for OAT, pseudo-genes are not transcribed or lead to non-functional proteins, DNA and amino acid sequence comparisons and phylogenetic analysis
it is believed that there is only one gene for OAT, pseudo-genes are not transcribed or lead to non-functional proteins, DNA and amino acid sequence comparisons and phylogenetic analysis