2.5.1.97: pseudaminic acid synthase

This is an abbreviated version!
For detailed information about pseudaminic acid synthase, go to the full flat file.

Reaction

Synonyms

NeuB3, PseG, PseI

ECTree

     2 Transferases

         2.5 Transferring alkyl or aryl groups, other than methyl groups

             2.5.1 Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)

                2.5.1.97 pseudaminic acid synthase

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Results	in table
1889	AA Sequence
2	Cloned(Commentary)
1	General Information
2	KM Value [mM]
5	Metals/Ions
2	Molecular Weight [Da]
2	Natural Substrates/ Products (Substrates)
2	Organism
4	Pathway
4	PDB ID
1	pH Optimum
2	Purification (Commentary)
1	Reaction
2	Reference
5	Substrates and Products (Substrate)
2	Subunits
3	Synonyms
1	Systematic Name
1	Turnover Number [1/s]

Substrates Products

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Substrates Products on EC 2.5.1.97 - pseudaminic acid synthase

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SUBSTRATE

PRODUCT                       

REACTION DIAGRAM

ORGANISM

UNIPROT

COMMENTARY
(Substrate)

LITERATURE
(Substrate)

COMMENTARY
(Product)

LITERATURE
(Product)

Reversibility
r=reversible
ir=irreversible
?=not specified

phosphoenolpyruvate + 2,4-diacetamido-2,4,6-trideoxy-beta-L-altropyranose + H2O

phosphate + 5,7-bis(acetylamino)-3,5,7,9-tetradeoxy-L-glycero-alpha-L-manno-2-nonulopyranosonic acid

4 entries

additional information

?

-

Campylobacter jejuni

-

N-acetyl-D-mannosamine can not replace 2,4-diacetamido-2,4,6-trideoxy-beta-L-altropyranose as a substrate indicating that the enzyme does not possess detectable sialic acid synthase activity

715481

-

-

?

phosphoenolpyruvate + 2,4-diacetamido-2,4,6-trideoxy-beta-L-altropyranose + H2O

phosphate + 5,7-bis(acetylamino)-3,5,7,9-tetradeoxy-L-glycero-alpha-L-manno-2-nonulopyranosonic acid

Campylobacter jejuni

-

pseudaminic acid biosynthesis

715481

-

-

?

phosphoenolpyruvate + 2,4-diacetamido-2,4,6-trideoxy-beta-L-altropyranose + H2O

phosphate + 5,7-bis(acetylamino)-3,5,7,9-tetradeoxy-L-glycero-alpha-L-manno-2-nonulopyranosonic acid

Campylobacter jejuni

-

phosphoenolpyruvate attacks the si-face of the aldehyde of 2,4-diacetamido-2,4,6-trideoxy-beta-L-altropyranose to generate an (S)-configuration at C-4. The proposed catalytic mechanism of pseudaminic acid synthase is the attack of the C-3 of PEP to the aldehyde of the open chain form of 6-deoxy-AltdiNAc and an attack of water to the C-2 of phosphoenolpyruvate to form a tetrahedral intermediate. The tetrahedral intermediate then collapses to release inorganic phosphate and form the open chain form of pseudaminic acid that cyclizes to the pyranose form in solution

715481

-

-

?

phosphoenolpyruvate + 2,4-diacetamido-2,4,6-trideoxy-beta-L-altropyranose + H2O

phosphate + 5,7-bis(acetylamino)-3,5,7,9-tetradeoxy-L-glycero-alpha-L-manno-2-nonulopyranosonic acid

Helicobacter pylori

-

pseudaminic acid biosynthesis

692521

-

-

?

phosphoenolpyruvate + 2,4-diacetamido-2,4,6-trideoxy-beta-L-altropyranose + H2O

phosphate + 5,7-bis(acetylamino)-3,5,7,9-tetradeoxy-L-glycero-alpha-L-manno-2-nonulopyranosonic acid

Helicobacter pylori

-

combining the five enzymes of pseudaminic acid biosynthesis (PseB, PseC, PseH, PseG, PseI) in a one-pot reaction results in 100% conversion of UDP-N-acetylglucosamine to pseudaminic acid after 20 h

692521

-

-

?