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2.5.1.94: adenosyl-chloride synthase

This is an abbreviated version!
For detailed information about adenosyl-chloride synthase, go to the full flat file.

Word Map on EC 2.5.1.94

Reaction

S-adenosyl-L-methionine
+
chloride
=
5-deoxy-5-chloroadenosine
 +
L-methionine

Synonyms

5'-chloro-5'-deoxyadenosine synthase, AclH, chlorinase, Cla1, ClA2, S-adenosyl-L-methionine-dependent chlorinase, SalL, SAM-dependent chlorinase

ECTree

     2 Transferases
         2.5 Transferring alkyl or aryl groups, other than methyl groups
             2.5.1 Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
                2.5.1.94 adenosyl-chloride synthase

Engineering

Engineering on EC 2.5.1.94 - adenosyl-chloride synthase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
G131S
-
the mutation results in instability and arrested chlorination
Y70T
-
the mutation leads to a 1000fold loss of chlorination activity
G131S
the mutation leads to the complete loss of all SalL halogenase activities and the inability to crystallize SalL
W129F
the mutant shows 52% chlorinase activity, 76% brominase activity, and 3% iodidase activity compared to the wild type enzyme
Y70T
the mutation results in a two-orders-of-magnitude reduction of SalL activity, the mutant shows 0.07% chlorinase activity, 0.1% brominase activity, and 0.4% iodidase activity compared to the wild type enzyme
Y70T/G131S
the mutant shows 0.3% chlorinase activity, 0.5% brominase activity, and no iodidase activity compared to the wild type enzyme
additional information