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2.5.1.9: riboflavin synthase

This is an abbreviated version!
For detailed information about riboflavin synthase, go to the full flat file.

Word Map on EC 2.5.1.9

Reaction

2 6,7-dimethyl-8-(1-D-ribityl)lumazine =

riboflavin
+
4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine

Synonyms

heavy riboflavin synthase, light riboflavin synthase, lumazine synthase/riboflavin synthase complex, RibD, RibE1, riboflavin synthase, riboflavin synthetase, riboflavine synthase, riboflavine synthetase, synthase, riboflavin

ECTree

     2 Transferases
         2.5 Transferring alkyl or aryl groups, other than methyl groups
             2.5.1 Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
                2.5.1.9 riboflavin synthase

Purification

Purification on EC 2.5.1.9 - riboflavin synthase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
HisTrap column chromatography and Superdex 200 gel filtration
partially
recombinant enzyme from Escherichia coli
-
recombinant enzyme from Escherichia coli to homogeneity
recombinant wild-type and mutant enzymes from Escherichia coli by ion exchange chromatography, ultrafiltration, gel filtration, and hydrophobic interaction chromatography
wild-type enzyme and mutants
-