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2.5.1.9: riboflavin synthase

This is an abbreviated version!
For detailed information about riboflavin synthase, go to the full flat file.

Word Map on EC 2.5.1.9

Reaction

2 6,7-dimethyl-8-(1-D-ribityl)lumazine =

riboflavin
+
4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine

Synonyms

heavy riboflavin synthase, light riboflavin synthase, lumazine synthase/riboflavin synthase complex, RibD, RibE1, riboflavin synthase, riboflavin synthetase, riboflavine synthase, riboflavine synthetase, synthase, riboflavin

ECTree

     2 Transferases
         2.5 Transferring alkyl or aryl groups, other than methyl groups
             2.5.1 Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
                2.5.1.9 riboflavin synthase

Cloned

Cloned on EC 2.5.1.9 - riboflavin synthase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloning of sequence segments of residues 1-97 and 101-213, and expression in Escherichia coli
-
DNA and amino acid sequence determination and analysis, functional expression in riboflavin-deficient Escherichia coli mutant BSV23
DNA and amino acid sequence determination and analysis, subcloning and expression in Escherichia coli strains M15 and XL1-Blue, wild-type and mutant enzymes
expressed in Escherichia coli BL21(DE3) cells
expressed in Nicotiana tabacum
-
expression in Escherichia coli
expression in Escherichia coli BL21(DE3) cells
-
expression of beta60 capsid
-
expression of wild-type and mutant enzymes in strain BL21(DE3)
-
optimization of expression in Escherichia coli by construction of a synthetic gene
optimization of expression in Escherichia coli by construction of a synthetic gene, phylogenetic analysis
phylogenetic analysis, highly effective expression in Escherichia coli by construction of a synthetic gene optimized for heterologous expression by exchange of 59 codons
-
recombinant N-terminal domain of riboflavin synthase is prepared
-
ribC hyperexpression strain, DNA sequence determination of mutants
-
wild-type enzyme and the four deletion constructs are individually co-expressed with His6-tagged lumazine synthase in Escherichia coli. Lumazine synthase forms cage complexes with the cognate riboflavin synthase from Aquifex aeolicus when both proteins are co-produced in the cytosol of Escherichia coli. A 12-amino acid-long peptide at the C terminus of riboflavin synthase serves as a specific localization sequence responsible for targeting the guest to the protein compartment
-