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2.5.1.78: 6,7-dimethyl-8-ribityllumazine synthase

This is an abbreviated version!
For detailed information about 6,7-dimethyl-8-ribityllumazine synthase, go to the full flat file.

Word Map on EC 2.5.1.78

Reaction

1-deoxy-L-glycero-tetrulose 4-phosphate
+
5-amino-6-(D-ribitylamino)uracil
=
6,7-dimethyl-8-(D-ribityl)lumazine
+ 2 H2O +
phosphate

Synonyms

6,7-dimethyl-8-(d-ribityl)lumazine synthase, 6,7-dimethyl-8-ribityllumazine synthase, 6,7-dimethyl-8-ribityllumazine synthase 1, 6,7-dimethyl-8-ribityllumazine synthase 2, 6,7-dimethyl-8-ribityllumazine-synthase, AaLS, BLS, DMRL synthase, DMRL synthase 2, heavy riboflavin synthase, LcLS1, LcLS2, lumazine synthase, lumazine synthase 1, lumazine synthase 2, lumazine synthase/riboflavin synthase complex, lumazinesynthase/riboflavin synthase complex, lumazinesynthase/riboflavin synthase complex, icosahedral capsid of 60 beta subunits enclosing a triplet of alpha subunits, luminazine synthase, MbtLS, MJ0303, Pbls, RIB4, ribE, RibH, ribH1, RibH1 protein, RibH2, type I lumazine synthase, type II lumazine synthase

ECTree

     2 Transferases
         2.5 Transferring alkyl or aryl groups, other than methyl groups
             2.5.1 Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
                2.5.1.78 6,7-dimethyl-8-ribityllumazine synthase

Purification

Purification on EC 2.5.1.78 - 6,7-dimethyl-8-ribityllumazine synthase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene bls, recombinant GST-tagged enzyme BLS and recombinant His-tagged BLS-Omp31 fusion protein from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography and nickel affinity chromatography, respectively, and tag removal using thrombin
gene RIB4A, recombinant His-tagged enzyme from Escherichia coli by nickel affinityy chromatography, gel filtration and ultrafiltration
overexpression of the RIB4 coding sequence in yeast cells under the control of the strong TEF1 promoter allowed ready purification of 6,7-dimethyl-8-ribityllumazine synthase to apparent homogeneity by a simple procedure
recombinant
recombinant Bs-l7/l12-BLS fusion protein from Escherichia coli strain BL21(DE3)
recombinant enzyme
recombinant enzyme BLS from Escherichia coli strain BL21 (DE3) by dialysis, anion exchange chromatography, and gel filtration
recombinant His-tagged fusion protein BLS-Stx2B by nickel affinity chromatography
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