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2.5.1.78: 6,7-dimethyl-8-ribityllumazine synthase

This is an abbreviated version!
For detailed information about 6,7-dimethyl-8-ribityllumazine synthase, go to the full flat file.

Word Map on EC 2.5.1.78

Reaction

1-deoxy-L-glycero-tetrulose 4-phosphate
+
5-amino-6-(D-ribitylamino)uracil
=
6,7-dimethyl-8-(D-ribityl)lumazine
 + 2 H2O +
phosphate

Synonyms

6,7-dimethyl-8-(d-ribityl)lumazine synthase, 6,7-dimethyl-8-ribityllumazine synthase, 6,7-dimethyl-8-ribityllumazine synthase 1, 6,7-dimethyl-8-ribityllumazine synthase 2, 6,7-dimethyl-8-ribityllumazine-synthase, AaLS, BLS, DMRL synthase, DMRL synthase 2, heavy riboflavin synthase, LcLS1, LcLS2, lumazine synthase, lumazine synthase 1, lumazine synthase 2, lumazine synthase/riboflavin synthase complex, lumazinesynthase/riboflavin synthase complex, lumazinesynthase/riboflavin synthase complex, icosahedral capsid of 60 beta subunits enclosing a triplet of alpha subunits, luminazine synthase, MbtLS, MJ0303, Pbls, RIB4, ribE, RibH, ribH1, RibH1 protein, RibH2, type I lumazine synthase, type II lumazine synthase

ECTree

     2 Transferases
         2.5 Transferring alkyl or aryl groups, other than methyl groups
             2.5.1 Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
                2.5.1.78 6,7-dimethyl-8-ribityllumazine synthase

Cloned

Cloned on EC 2.5.1.78 - 6,7-dimethyl-8-ribityllumazine synthase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
construction of a fusion protein with enzyme BLS and inner core domain (VP8d) of VP8 spike protein from bovine rotavirus, subcloning in Escherichia coli, expression of the BLS-VP8d fusion protein in Nicotiana tabacum cv. Petit Havana leaf chloroplasts to evaluate the possible use of this antigen for vaccine development
expression in Escherichia coli
expression in Escherichia coli. The recombinant protein is soluble only under reducing conditions, but alkylation with iodoacetamide renders it soluble in non-reducing media
expression of beta60 capsid
-
expression of beta60 capsid carying lumazine synthase activity
-
gene bls, recombinant expression of GST-tagged enzyme in Escherichia coli strain BL21(DE3), recombinant expression of enzyme BLS fused to protein omp31 as His-tagged protein in Escherichia coli strain BL21(DE3)
gene LcLS1, DNA and amino acid sequence determination and analysis, sequence comparisons, quantitative real-time PCR expression analysis
gene LcLS2, DNA and amino acid sequence determination and analysis, sequence comparisons, quantitative real-time PCR expression analysis
gene RIB4A, recombinant expression of His-tagged enzyme in Escherichia coli
gene ribH2, recombinant coexpression of protein rL7/L12 with enzyme BLS as fusion protein in Escherichia coli strain BL21(DE3). The Bs-l7/l12 gene is cloned into a specific pET-28-a expression vector, which is inserted by the Brucella lumazine synthase enzyme gene, to form the pET-28-a-l7/l12-bls fusion gene expression vector
lumazine synthase forms cage complexes with the cognate riboflavin synthase from Aquifex aeolicus when both proteins are co-produced in the cytosol of Escherichia coli. A 12-amino acid-long peptide at the C terminus of riboflavin synthase serves as a specific localization sequence responsible for targeting the guest to the protein compartment
recombinant expression of a His-tagged fusion protein BLS-Stx2B formed by the enzyme and the Shiga toxin B subunit
-
recombinant expression of enzyme BLS in Escherichia coli strain BL21 (DE3)
recombinant expression of the enzyme in Escherichia coli, and of the enzyme fused to Shiga toxin 2 subunit B, resulting in the BLS-Stx2B chimera, in Escherichia coli strain JM109
-