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dimethylallyl diphosphate + A37 nucleoside
N6-(D2-isopentenyl)adenine + diphosphate
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dimethylallyl diphosphate + adenine37 in tRNA
diphosphate + N6-dimethylallyladenine37 in tRNA
dimethylallyl diphosphate + adenine37 in tRNA([Ser]Sec)
diphosphate + N6-dimethylallyladenine37 in tRNA([Ser]Sec)
dimethylallyl diphosphate + adenine37 in tRNAPhe
diphosphate + N6-dimethylallyladenine37 in tRNAPhe
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enzyme transfers the dimethylallyl moiety of dimethylallyl diphosphate to A37, located adjacent to the anticodon in undermodified tRNAPhe
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?
dimethylallyl diphosphate + adenine37 in tRNATrp
diphosphate + N6-dimethylallyladenine37 in tRNATrp
dimethylallyl diphosphate + adenosine 37 in tRNA
N6-(dimethylallyl)adenosine37 in tRNA + diphosphate
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dimethylallyl diphosphate + adenosine 37 in tRNA-Phe
N6-(dimethylallyl)adenosine37 in tRNAPhe + diphosphate
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dimethylallyl diphosphate + adenosine 37 in tRNACys
N6-(dimethylallyl)adenosine37 in tRNACys + diphosphate
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dimethylallyl diphosphate + adenosine 37 in tRNASer
N6-(dimethylallyl)adenosine37 in tRNASer + diphosphate
dimethylallyl diphosphate + adenosine 37 in tRNATrpCCA
N6-(dimethylallyl)adenosine37 in tRNATrpCCA + diphosphate
dimethylallyl diphosphate + adenosine 37 in tRNATyr
N6-(dimethylallyl)adenosine37 in tRNATyr + diphosphate
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dimethylallyl diphosphate + adenosine 37 in tRNATyrGUA
N6-(dimethylallyl)adenosine37 in tRNATyrGUA + diphosphate
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dimethylallyl diphosphate + adenosine37 in tRNATrpCCA
N6-(dimethylallyl)adenosine37 in tRNATrpCCA + diphosphate
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dimethylallyl diphosphate + GCGGACUCAAAAUCCGC
diphosphate + GCGGACUCAAAAUCCGC containing 6-dimethylallyladenosine
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chemically synthesized 17-base RNA oligoribonucleotide minihelix based on variations in the anticodon stem-loop of unmodified Escherichia coli tRNAPhe
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dimethylallyl diphosphate + GGCCAUUGAAAAUGGCC
diphosphate + GGCCAUUGAAAAUGGCC containing 6-dimethylallyladenosine
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chemically synthesized 17-base RNA oligoribonucleotide minihelix based on variations in the anticodon stem-loop of unmodified Escherichia coli tRNAPhe
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dimethylallyl diphosphate + GGGAAUUGAAAAUUCCC
diphosphate + GGCCAUUGAAAAUGGCC containing 6-dimethylallyladenosine
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chemically synthesized 17-base RNA oligoribonucleotide minihelix based on variations in the anticodon stem-loop of unmodified Escherichia coli tRNAPhe
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dimethylallyl diphosphate + GGGCAUUGAAAAUGCCC
diphosphate + GGGCAUUGAAAAUGCCC containing 6-dimethylallyladenosine
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chemically synthesized 17-base RNA oligoribonucleotide minihelix based on variations in the anticodon stem-loop of unmodified Escherichia coli tRNAPhe
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dimethylallyl diphosphate + GGGGAUUGAAAAAGGGG
diphosphate + GGGGAUUGAAAAAGGGG containing 6-dimethylallyladenosine
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chemically synthesized 17-base RNA random coil oligoribonucleotide based on variations in the anticodon stem-loop of unmodified Escherichia coli tRNAPhe
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dimethylallyl diphosphate + GGGGAUUGAAAAUCCCC
diphosphate + GGGGAUUGAAAAAGGGG containing 6-dimethylallyladenosine
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chemically synthesized 17-base RNA oligoribonucleotide minihelix based on variations in the anticodon stem-loop of unmodified Escherichia coli tRNAPhe
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dimethylallyl diphosphate + GGGGAUUGAAAGUCCCC
diphosphate + GGGGAUUGAAAGUCCCC containing 6-dimethylallyladenosine
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chemically synthesized 17-base RNA oligoribonucleotide minihelix based on variations in the anticodon stem-loop of unmodified Escherichia coli tRNAPhe
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dimethylallyl diphosphate + GGGGAUUGAGAAUCCCC
diphosphate + GGGGAUUGAGAAUCCCC containing 6-dimethylallyladenosine
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chemically synthesized 17-base RNA oligoribonucleotide minihelix based on variations in the anticodon stem-loop of unmodified Escherichia coli tRNAPhe
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dimethylallyl diphosphate + GGGGGUUGAAAACCCCC
diphosphate + GGGGGUUGAAAACCCCC containing 6-dimethylallyladenosine
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chemically synthesized 17-base RNA oligoribonucleotide minihelix based on variations in the anticodon stem-loop of unmodified Escherichia coli tRNAPhe
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dimethylallyl diphosphate + oligo-RNA
oligo-RNA containing N6-(dimethylallyl)adenosine + diphosphate
substrates are 17-nt or 19-nt synthetic oligo-RNAs representing anticondon stem-loops as minihelix analogs of tRNAs. Only the anticodon stem-loops with a predicted stem of 6 bp are efficiently modified
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dimethylallyl diphosphate + seventeen-base RNA oligonucleotide
diphosphate + seventeen-base RNA oligonucleotide containing 6-dimethylallyladenosine
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sequence 5'GCGGACUCAAAAUCCGC3', RNA oligonucleotide based on the unmodified stem-loop region of tRNAPhe
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dimethylallyl diphosphate + tRNATrpCCA
tRNATrpCCA containing N6-(dimethylallyl)adenosine + diphosphate
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additional information
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dimethylallyl diphosphate + adenine37 in tRNA
diphosphate + N6-dimethylallyladenine37 in tRNA
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dimethylallyl diphosphate + adenine37 in tRNA
diphosphate + N6-dimethylallyladenine37 in tRNA
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dimethylallyl diphosphate + adenine37 in tRNA
diphosphate + N6-dimethylallyladenine37 in tRNA
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dimethylallyl diphosphate + adenine37 in tRNA
diphosphate + N6-dimethylallyladenine37 in tRNA
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dimethylallyl diphosphate + adenine37 in tRNA
diphosphate + N6-dimethylallyladenine37 in tRNA
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regulates global physiology by unknown mechanisms
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dimethylallyl diphosphate + adenine37 in tRNA
diphosphate + N6-dimethylallyladenine37 in tRNA
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dimethylallyl diphosphate + adenine37 in tRNA
diphosphate + N6-dimethylallyladenine37 in tRNA
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tRNA lacking the isopentenyl modification normally present in vivo
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dimethylallyl diphosphate + adenine37 in tRNA
diphosphate + N6-dimethylallyladenine37 in tRNA
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specific for DELTA2-isopentenyl phosphate
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dimethylallyl diphosphate + adenine37 in tRNA
diphosphate + N6-dimethylallyladenine37 in tRNA
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recognition mode of substrate, structural features of substrate tRNA
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dimethylallyl diphosphate + adenine37 in tRNA
diphosphate + N6-dimethylallyladenine37 in tRNA
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mycoplasma species (kid) tRNA
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dimethylallyl diphosphate + adenine37 in tRNA
diphosphate + N6-dimethylallyladenine37 in tRNA
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no reaction with DELTA3-isopentenyl diphosphate
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dimethylallyl diphosphate + adenine37 in tRNA
diphosphate + N6-dimethylallyladenine37 in tRNA
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dimethylallyl diphosphate + adenine37 in tRNA
diphosphate + N6-dimethylallyladenine37 in tRNA
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ir
dimethylallyl diphosphate + adenine37 in tRNA
diphosphate + N6-dimethylallyladenine37 in tRNA
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dimethylallyl diphosphate + adenine37 in tRNA
diphosphate + N6-dimethylallyladenine37 in tRNA
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ir
dimethylallyl diphosphate + adenine37 in tRNA
diphosphate + N6-dimethylallyladenine37 in tRNA
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specific for DELTA2-isopentenyl phosphate
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dimethylallyl diphosphate + adenine37 in tRNA
diphosphate + N6-dimethylallyladenine37 in tRNA
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no reaction with DELTA3-isopentenyl diphosphate
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dimethylallyl diphosphate + adenine37 in tRNA
diphosphate + N6-dimethylallyladenine37 in tRNA
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no reaction with homologous native tRNA or homologous, permanganate-treated tRNA (permanganate specifically cleaves the DELTA2-isopentenyl groups of tRNA leaving adenosine residues)
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dimethylallyl diphosphate + adenine37 in tRNA
diphosphate + N6-dimethylallyladenine37 in tRNA
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the enzyme from both yeast and rat liver catalyzes a significant incorporation of DELTA2-isopentenyl groups into untreated E. coli B tRNA
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dimethylallyl diphosphate + adenine37 in tRNA
diphosphate + N6-dimethylallyladenine37 in tRNA
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biosynthesis of N6-(DELTA2-isopentenyl)adenosine which several species of tRNA contain adjacent to the 3'-end of the anticodon
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dimethylallyl diphosphate + adenine37 in tRNA
diphosphate + N6-dimethylallyladenine37 in tRNA
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dimethylallyl diphosphate + adenine37 in tRNA
diphosphate + N6-dimethylallyladenine37 in tRNA
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dimethylallyl diphosphate + adenine37 in tRNA
diphosphate + N6-dimethylallyladenine37 in tRNA
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dimethylallyl diphosphate + adenine37 in tRNA
diphosphate + N6-dimethylallyladenine37 in tRNA
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no reaction with DELTA3-isopentenyl diphosphate
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dimethylallyl diphosphate + adenine37 in tRNA([Ser]Sec)
diphosphate + N6-dimethylallyladenine37 in tRNA([Ser]Sec)
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dimethylallyl diphosphate + adenine37 in tRNA([Ser]Sec)
diphosphate + N6-dimethylallyladenine37 in tRNA([Ser]Sec)
tRNA[Ser]Sec modification assay with [14C]DMAPP and in vitro transcribed tRNA[Ser]Sec. Incubation of in vitro transcribed tRNA[Ser]Sec with recombinant Trit1 transfers [14C]dimethylallyl diphosphate to tRNA[Ser]Sec reveals that 37A>G tRNA[Ser]Sec is resistant to isopentenylation by Trit1
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dimethylallyl diphosphate + adenine37 in tRNATrp
diphosphate + N6-dimethylallyladenine37 in tRNATrp
isopentenylaion of the A37 within the ACSLTrp sequence, detailed spectrometrical reaction analysis, overview
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dimethylallyl diphosphate + adenine37 in tRNATrp
diphosphate + N6-dimethylallyladenine37 in tRNATrp
isopentenylaion of the A37 within the ACSLTrp sequence, detailed spectrometrical reaction analysis, overview
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dimethylallyl diphosphate + adenosine 37 in tRNASer
N6-(dimethylallyl)adenosine37 in tRNASer + diphosphate
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dimethylallyl diphosphate + adenosine 37 in tRNASer
N6-(dimethylallyl)adenosine37 in tRNASer + diphosphate
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dimethylallyl diphosphate + adenosine 37 in tRNATrpCCA
N6-(dimethylallyl)adenosine37 in tRNATrpCCA + diphosphate
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dimethylallyl diphosphate + adenosine 37 in tRNATrpCCA
N6-(dimethylallyl)adenosine37 in tRNATrpCCA + diphosphate
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additional information
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enzyme does not bind dimethylallyl diphosphate in absence of tRNA
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additional information
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The A36-A37-A38 motif, which is completely conserved in tRNAs modified by the enzyme, is important for modification. A helix-loop structure is essential for recognition
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additional information
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enzyme recognizes the tRNA substrate through indirect sequence readout. The targeted nucleotide A37 flips out from the anticodon loop of tRNA and flips into a channel in DMATase, where it meets its reaction partner dimethylallyl diphosphate, which enters the channel from the opposite end. Structural changes accompanying the transfer reaction result in disengagement of DMATase-tRNA interaction near the reaction center
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additional information
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enzyme recognizes the tRNA substrate through indirect sequence readout. The targeted nucleotide A37 flips out from the anticodon loop of tRNA and flips into a channel in DMATase, where it meets its reaction partner dimethylallyl diphosphate, which enters the channel from the opposite end. Structural changes accompanying the transfer reaction result in disengagement of DMATase-tRNA interaction near the reaction center
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additional information
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the minimal substrate for bacterial MiaA is the anticodon stem loop (ACSL) of targeted tRNA molecules
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additional information
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the minimal substrate for bacterial MiaA is the anticodon stem loop (ACSL) of targeted tRNA molecules
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additional information
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anticondon stem-loops with substitutions as in Ser-G36, Ser-G37, and Ser-G36,G38 greatly diminish activity, and Ser-C38 and Ser-U38 are not modified, while Ser-G38 is a substrate
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additional information
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anticondon stem-loops with substitutions as in Ser-G36, Ser-G37, and Ser-G36,G38 greatly diminish activity, and Ser-C38 and Ser-U38 are not modified, while Ser-G38 is a substrate
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