2.5.1.75: tRNA dimethylallyltransferase

This is an abbreviated version!
For detailed information about tRNA dimethylallyltransferase, go to the full flat file.

Reaction

Synonyms

A37 isopentenyltransferase, alr5266, DELTA2-isopentenyl pyrophosphate:transfer ribonucleic acid DELTA2-isopentenyltransferase, DELTA2-isopentenyl pyrophosphate:tRNA-DELTA2-isopentenyl transferase, dimethylallyl (DELTA2-isopentenyl)diphosphate-tRNA transferase, dimethylallyl(D2-isopentenyl) diphosphate:tRNA transferase, dimethylallyl:tRNA[Ser]Sec transferase, DMAPP:tRNA[Ser]Sec transferase, DMATase, EC 2.5.1.8, IPT, IPT2, IPT9, isopentenyl transferase, isopentenyltransferase, isopentenyltransferase, transfer ribonucleate, MhIPT3, MiaA, Mod5, NoIPT2, prenyltransferase, Tit1, transfer ribonucleate isopentenyltransferase, TRIT1, tRNA IPT, tRNA isopentenyltransferase, tRNA([Ser]Sec)-isopentenyl transferase, tRNA-IPT, tRNA-IPT1

ECTree

     2 Transferases

         2.5 Transferring alkyl or aryl groups, other than methyl groups

             2.5.1 Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)

                2.5.1.75 tRNA dimethylallyltransferase

Advanced search results

Do not include text mining results

Include results (more...)

Include results (more...)

Results	in table
43495	AA Sequence
4	Application
1	CAS Registry Number
11	Cloned(Commentary)
2	Crystallization (Commentary)
202	Disease/ Diagnostics
1	Expression
9	General Information
1	General Stability
11	Inhibitors
42	KM Value [mM]
4	Localization
13	Metals/Ions
6	Molecular Weight [Da]
7	Natural Substrates/ Products (Substrates)
34	Organism
4	Pathway
7	pH Optimum
2	pH Range
43	Protein Variants
9	Purification (Commentary)
5	Reaction
1	Reaction Type
34	Reference
12	Source Tissue
5	Specific Activity [micromol/min/mg]
5	Storage Stability
59	Substrates and Products (Substrate)
2	Subunits
41	Synonyms
1	Systematic Name
3	Temperature Optimum [°C]
19	Turnover Number [1/s]

General Information

print visible entries

print all entries

Go back to the abbreviated version
of the Enzyme Summary Page.

General Information on EC 2.5.1.75 - tRNA dimethylallyltransferase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.

top print hide show all columns Go to General Information Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

GENERAL INFORMATION

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

evolution

2 entries

malfunction

Mus musculus

Q80UN9

knockdown of Trit1 reduces expression of selenoproteins. Trit1 proteins carrying the D55G or T32A mutations exhibit significantly reduced specific activity of 50% and 10%of wild-type respectively. Overexpression of TRIT1 does not increase selenoprotein expression in HepG2 cells

737635

metabolism

2 entries

physiological function

4 entries

evolution

Salmonella enterica

P37724

while absent in eukaryotes, some facultative bacteria use a non-heme diiron enzyme [MiaE, 2-methylthio-N6-isopentenyladenosine(37)-tRNA monooxygenase] to catalyze the O2-dependent hydroxylation of ms2i6A37 to produce 2-methylthio-N6-(4-hydroxyisopentenyl)-adenosine (ms2io6A37), comparison of MiaA and MiaE catalytic reactions, overview

737708

evolution

Salmonella enterica ATCC 700720

-

while absent in eukaryotes, some facultative bacteria use a non-heme diiron enzyme [MiaE, 2-methylthio-N6-isopentenyladenosine(37)-tRNA monooxygenase] to catalyze the O2-dependent hydroxylation of ms2i6A37 to produce 2-methylthio-N6-(4-hydroxyisopentenyl)-adenosine (ms2io6A37), comparison of MiaA and MiaE catalytic reactions, overview

-

metabolism

Salmonella enterica

P37724

the enzyme is involved in the hypermodification enzyme pathway for 2-methylthio-N6-(4-hydroxyisopentenyl)-adenosine (ms2io6A37) synthesis. within the anticodon stem loop of tRNA, nucleotide modifications are frequently observed at position 34 (the wobble position) or position 37 (3' adjacent to the anticodon), nearly all eukaryotic and bacterial tRNAs that read codons starting with U are modified by the enzymes MiaA and MiaB and contain the ms2i6A modification

737708

metabolism

Salmonella enterica ATCC 700720

-

the enzyme is involved in the hypermodification enzyme pathway for 2-methylthio-N6-(4-hydroxyisopentenyl)-adenosine (ms2io6A37) synthesis. within the anticodon stem loop of tRNA, nucleotide modifications are frequently observed at position 34 (the wobble position) or position 37 (3' adjacent to the anticodon), nearly all eukaryotic and bacterial tRNAs that read codons starting with U are modified by the enzymes MiaA and MiaB and contain the ms2i6A modification

-

physiological function

Salmonella enterica

P37724

enzyme MiaA catalyzes the nucleophilic substitution of the dimethylallyl group from dimethylallyl diphosphate (DMAPP) to the exocyclic 6-amino nitrogen of A37 (N6) to yield N6-isopentenyladenosine (i6A37) with release of inorganic diphosphate

737708

physiological function

Mus musculus

Q80UN9

mammalian Trit1 is a tRNA([Ser]Sec)-isopentenyl transferase required for full selenoprotein expression, full expression of selenoproteins requires modification of tRNA[Ser]Sec, including N6-isopentenylation of base A37

737635

physiological function

Homo sapiens

Q9H3H1

Trit1 exhibits substrate-specific restriction against Schizosaccharomyces pombe cytosolic tRNATrpCCA-A37-A38. Position 32 of tRNATrpCCA may be a conditional determinant for substrate-specific i6A37 modification by Trit1

760010

physiological function

Salmonella enterica ATCC 700720

-

enzyme MiaA catalyzes the nucleophilic substitution of the dimethylallyl group from dimethylallyl diphosphate (DMAPP) to the exocyclic 6-amino nitrogen of A37 (N6) to yield N6-isopentenyladenosine (i6A37) with release of inorganic diphosphate

-