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2.5.1.73: O-phospho-L-seryl-tRNA:Cys-tRNA synthase

This is an abbreviated version!
For detailed information about O-phospho-L-seryl-tRNA:Cys-tRNA synthase, go to the full flat file.

Word Map on EC 2.5.1.73

Reaction

O-phospho-L-seryl-tRNACys
+
Sulfide
=
L-Cysteinyl-tRNACys
 +
phosphate

Synonyms

MJ1678, Sep-tRNA-Cys-tRNA synthase, Sep-tRNA:Cys-tRNA synthase, SepCysS

ECTree

     2 Transferases
         2.5 Transferring alkyl or aryl groups, other than methyl groups
             2.5.1 Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
                2.5.1.73 O-phospho-L-seryl-tRNA:Cys-tRNA synthase

Engineering

Engineering on EC 2.5.1.73 - O-phospho-L-seryl-tRNA:Cys-tRNA synthase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C113A
activity similar to wild-type
C209A
activity similar to wild-type
C272A
loss of the ability to complement an Escherichia coli selA knockout strain, which cannot produce active formate dehydrogenase H due to the lack of selenocysteine incorporation
C272S
complete loss of activity
C64A
loss of the ability to complement an Escherichia coli selA knockout strain, which cannot produce active formate dehydrogenase H due to the lack of selenocysteine incorporation
C64S
complete loss of activity
C67A
loss of the ability to complement an Escherichia coli selA knockout strain, which cannot produce active formate dehydrogenase H due to the lack of selenocysteine incorporation
C67S
complete loss of activity
D182A
107% of wild-type activity
H126A
complete loss of activity
H233A
complete loss of activity
H325A
124% of wild-type activity
K234A
K265A
complete loss of activity
K354A/R356A
92% of wild-type activity
K370A
33% of wild-type activity
N187A
85% of wild-type activity
N208A
101% of wild-type activity
R102A
complete loss of activity
R292A
99% of wild-type activity
R96A
89% of wild-type activity
S231A
complete loss of activity
Y127A
35% of wild-type activity
C113A
-
activity similar to wild-type
-
C272A
-
loss of the ability to complement an Escherichia coli selA knockout strain, which cannot produce active formate dehydrogenase H due to the lack of selenocysteine incorporation
-
C64A
-
loss of the ability to complement an Escherichia coli selA knockout strain, which cannot produce active formate dehydrogenase H due to the lack of selenocysteine incorporation
-
C67A
-
loss of the ability to complement an Escherichia coli selA knockout strain, which cannot produce active formate dehydrogenase H due to the lack of selenocysteine incorporation
-
K234A
-
complete loss of activtiy
-
additional information