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2.5.1.73: O-phospho-L-seryl-tRNA:Cys-tRNA synthase

This is an abbreviated version!
For detailed information about O-phospho-L-seryl-tRNA:Cys-tRNA synthase, go to the full flat file.

Word Map on EC 2.5.1.73

Reaction

O-phospho-L-seryl-tRNACys
+
Sulfide
=
L-Cysteinyl-tRNACys
 +
phosphate

Synonyms

MJ1678, Sep-tRNA-Cys-tRNA synthase, Sep-tRNA:Cys-tRNA synthase, SepCysS

ECTree

     2 Transferases
         2.5 Transferring alkyl or aryl groups, other than methyl groups
             2.5.1 Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
                2.5.1.73 O-phospho-L-seryl-tRNA:Cys-tRNA synthase

Crystallization

Crystallization on EC 2.5.1.73 - O-phospho-L-seryl-tRNA:Cys-tRNA synthase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
purified recombinant wild-type SepVysS1 and selenomethionine-labeled SepCysS1, hanging-drop vapor diffusion method, 0.001 ml protein solution is mixed with 0.001 ml reservoir solution containing 80 mM sodium acetate buffer, pH 4.4, 160 mM NaCl, and 1.00 M ammonium sulfate, 20°C, equilibration against 0.5 ml reservoir solution, cryoprotection by 25% v/v glycerol, X-ray diffraction structure determination and analysis at 2.4-3.2 A resolution, modeling
crystal structure of a subcomplex of the transsulfursome with tRNACys (SepCysS-SepCysE-tRNACys), which is involved in the second reaction step of the indirect pathway, to 2.6 A resolution. Space group P6522, with unit-cell parameters a = b = 107.2, c = 551.1 A. Two heterodimers of a synthase SepCysS dimer, an enhancer SepCysE dimer and one tRNACys are observed in the asymmetric unit
crystal structure of the complex of the enzyme with translation factor SepCysE, 2.8 A resolution
structure of the SepCysE-SepCysS-tRNACys ternary complex, at a resolution of 2.6 A by molecular replacement. SepCysS recognizes the discriminator base U73 of tRNACys