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2.5.1.7: UDP-N-acetylglucosamine 1-carboxyvinyltransferase

This is an abbreviated version!
For detailed information about UDP-N-acetylglucosamine 1-carboxyvinyltransferase, go to the full flat file.

Word Map on EC 2.5.1.7

Reaction

phosphoenolpyruvate
+
UDP-N-acetyl-alpha-D-glucosamine
=
phosphate
 +
UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine

Synonyms

enol-pyruvyltransferase, enolpyruvyl UDP-GlcNAc synthase, enoylpyruvate transferase, enoylpyruvatetransferase, EPT, More, MurA, MurA enzyme, MurA transferase, MurAA, MurZ, phosphoenolpyruvate-UDP-acetylglucosamine-3-enolpyruvyltransferase, phosphoenolpyruvate:UDP-2-acetamido-2-deoxy-D-glucose 2-enoyl-1-carboxyethyltransferase, phosphoenolpyruvate:uridine diphosphate N-acetylglucosamine enolpyruvyltransferase, phosphoenolpyruvate:uridine-5'-diphospho-N-acetyl-2-amino-2-deoxyglucose-3-enolpyruvyltransferase, phosphopyruvate-uridine diphosphoacetylglucosamine pyruvatetransferase, pyruvate-UDP-acetylglucosamine transferase, pyruvate-uridine diphospho-N-acetyl-glucosamine transferase, pyruvate-uridine diphospho-N-acetylglucosamine transferase, pyruvatetransferase, phosphoenolpyruvate-uridine diphosphoacetylglucosamine, pyruvic-uridine diphospho-N-acetylglucosaminyltransferase, UDP-GlcNAc enolpyruvyl transferase, UDP-N-acetylglucosamine 1-carboxyvinyl transferase, UDP-N-acetylglucosamine 1-carboxyvinyl-transferase, UDP-N-acetylglucosamine 1-carboxyvinyltransferase, UDP-N-acetylglucosamine enolpyruvyl transferase, UDP-N-acetylglucosamine enolpyruvyle transferase, UDP-N-acetylglucosamine enolpyruvyltransferase, UDP-N-acetylglucosamine enoylpyruvyltransferase, UDP-N-acetylglucosamine-enolpyruvyl transferase, UDP-NAG enolpyruvyl transferase, UNAG enolpyruvyl transferase

ECTree

     2 Transferases
         2.5 Transferring alkyl or aryl groups, other than methyl groups
             2.5.1 Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
                2.5.1.7 UDP-N-acetylglucosamine 1-carboxyvinyltransferase

Cloned

Cloned on EC 2.5.1.7 - UDP-N-acetylglucosamine 1-carboxyvinyltransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloning and expression of MurA using pMAL expression system in frame with the fusion maltose binding protein in Escherichia coli BL21
coding region of Haemophilus influenzae MurA is cloned into the NdeI and XhoI sites of pET21a to generate an expression vector for MurA, the expression vector is transformed into Escherichia coli Rosetta2 (DE3), and the recombinant MurA is expressed
DNA and amino acid sequence determination and analysis, chromosome mapping at 69.3 min, overexpression in Escherichia coli strain JLM 16
DNA and amino acid sequence determination, functional overexpression in Escherichia coli strain JM105
expressed in an Enterococcus faecalis mutant lacking IreK (formerly PrkC), a key kinase required for cephalosporin resistance, and in Escherichia coli BL21(DE3) cells
-
expressed in Escherichia coli
-
expressed in Escherichia coli BL21 cells
expressed in Escherichia coli BL21 Star (DE3) cells
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli DH5alpha cells
expressed in Escherichia coli Rosetta(DE3)pLysS cells
-
expressed in Escherichia coli Rosetta2(DE3) cells
-
expressed in Escherichia coli STBL2-DE3 cells
expression from plasmid in Escherichia coli deletion mutant, functional complementation
expression in Escherichia coli
expression of wild-type and mutants in Escherichia coli
for the expression of AbMurA, the plasmid is introduced into C41(DE3) cells
-
gene murA, DNA sequence analysis, expression in Escherichia coli under control of the arabinose-inducible, glucose-repressible ara promotor, functional complementation, necessary for viability, of a enzyme-deficient Escherichia coli murA null-mutant, impartment of fosfomycin resistance to the Escherichia coli cell
-
genes murA1 and murA2, DNA and amino acid sequence determination, overexpression of both isoforms in Escherichia coli BL21(DE3)
-
overexpression in Escherichia coli
-
overexpression in Escherichia coli with a 6-His tag
overexpression of wild type MurA
the murA gene is cloned and overexpressed in Escherichia coli with a C-terminal 6-His tag
-
the murZ gene is cloned and overexpressed in Escherichia coli with a C-terminal 6-His tag
-