2.5.1.67: chrysanthemyl diphosphate synthase

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For detailed information about chrysanthemyl diphosphate synthase, go to the full flat file.

Word Map on EC 2.5.1.67

2.5.1.67

pyrethrins

insecticides

monoterpene

irregular

tanacetum

flowers

farnesyl

isoprenoid

pyrethrum

cyclopropanation

cinerariifolium

dmapp

monoterpenoids

trichomes

geranyl

isopentenyl

emit

asteraceae

lavandulyl

squalene

synthesis

terpene

gpp

head-to-tail

prenyltransferase

artemisia

tridentata

phytoene

plastidial

trichome-specific

headspace

Reaction

2 dimethylallyl diphosphate =

Synonyms

CDS, CDS_CCI2, chrysanthemol synthase, chrysanthemyl diphosphate synthase, CPP, CPP synthase, CPPase, FDS-5

ECTree

2 Transferases

2.5 Transferring alkyl or aryl groups, other than methyl groups

2.5.1 Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)

2.5.1.67 chrysanthemyl diphosphate synthase

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Results	in table
5	AA Sequence
2	Application
1	CAS Registry Number
6	Cloned(Commentary)
2	Expression
9	General Information
1	Inhibitors
1	kcat/KM [mM/s]
5	KM Value [mM]
5	Localization
5	Metals/Ions
4	Molecular Weight [Da]
12	Natural Substrates/ Products (Substrates)
14	Organism
1	Pathway
2	PDB ID
4	pH Optimum
1	pH Range
2	Posttranslational Modification
7	Protein Variants
3	Purification (Commentary)
2	Reaction
1	Reaction Type
12	Reference
7	Source Tissue
1	Specific Activity [micromol/min/mg]
1	Storage Stability
27	Substrates and Products (Substrate)
2	Subunits
14	Synonyms
1	Systematic Name
4	Temperature Optimum [°C]
2	Turnover Number [1/s]

Purification

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Purification on EC 2.5.1.67 - chrysanthemyl diphosphate synthase

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native enzyme from immature flowers by ammonium sulfate fractionation, anion exchange and hydrophobic interaction chromatography, followed by another step of anion chromatography and hydroxylapatite chromatography to apparent homogeneity, recombinant N-terminally His6-tagged enzyme from Escherichia coli strain XA90 by ammonium sulfate fractionation and nickel affinity chromatography

Tanacetum cinerariifolium

P0C565

670719

recombinant N-terminally His-tagged enzyme from Escherichia coli strain BL21(AI) by nickel affinity chromatography and ultrafiltration

Tanacetum cinerariifolium

L7RFF8

738635

recombinant N-terminally His6-tagged enzyme from Escherichia coli strain XA90 by nickel affinity chromatography to over 95% purity

Artemisia spiciformis

Q7XYS8

669199