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2.5.1.61: hydroxymethylbilane synthase

This is an abbreviated version!
For detailed information about hydroxymethylbilane synthase, go to the full flat file.

Word Map on EC 2.5.1.61

Reaction

4 porphobilinogen +

H2O
=
Hydroxymethylbilane
 + 4 NH3

Synonyms

(HMB)-synthase, AN0121.3, EC 4.3.1.8, HemC, hepatic porphobilinogen deaminase, HMB synthase, HMB-S, HMBS, HMBS1a-synthase, HMBS1b-synthase, HMBS2a-synthase, HMBS2b-synthase, human non-erythropoietic PBGD isoform, human PBGD, human porphobilinogen deaminase, hydroxymethylbilane synthase, More, PBG deaminase, PBG-D, PBG-deaminase, PBGD, PGB-D, porphobilinogen ammonia-lyase (polymerizing), porphobilinogen deaminase, pre-uroporphyrinogen synthase, preuroporphyrinogen synthase, preuroporphyrinogen synthetase, synthase, uroporphyrinogen I, uPBGD, UPGI-S, URO-S, urogenI synthase, uroporphyrinogen I synthase, uroporphyrinogen I synthetase, uroporphyrinogen synthase, uroporphyrinogen synthetase

ECTree

     2 Transferases
         2.5 Transferring alkyl or aryl groups, other than methyl groups
             2.5.1 Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
                2.5.1.61 hydroxymethylbilane synthase

Storage Stability

Storage Stability on EC 2.5.1.61 - hydroxymethylbilane synthase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-15°C, purified enzyme rapidely loses its activity
-
-20°C or 4°C, unstable, loss of activity after several days
-
-20°C, crude plastide lysate is stable for at least 2 weeks
-
-20°C, forms A to E, stable for up to 18 months in 10 mM potassium phosphate buffer, pH 8.0, containing 0.2 mM dithioerythritol
-
-20°C, stable for over 1 year
-
-80°C, 50 mM Tris buffer, pH 7.5, 5% glycerol, and 5 mM beta-mercaptoethanol, 1 year, remains stable
0-4°C, less than 5% loss of activity over 10 days
-
0°C, stable in solution at pH 7.5 and 9.5 for 3 weeks
-
4°C, 50 mM Tris buffer, pH 7.5, 5% glycerol, and 5 mM beta-mercaptoethanol, 1 week, without significant change of activity
4°C, purified enzyme stable for more than a month
-
erythrocytes lysates stored in 1 mM potassium phosphate, pH 7.6, containing 0.05% Trition X-100, 1 mM dithiothreitol and 1 mM MgCl2, no or little loss of activity after 1 year
-
purified enzyme loses its activity when it is frozen
-
stored in 0.1 M Tris chloride, pH 8.5, containing 30% glycerol and 0.01 M 2-mercaptoethanol. Under these conditions 50% loss of activity after 3 weeks
-