CAAX geranylgeranyltransferase, CaaX prenyltransferase, CaGGTase-I, CDC43, geranylgeranyl protein transferase type I, geranylgeranyltransferase I, geranylgeranyltransferase type I, geranylgeranyltransferase type-I, geranylgeranyltransferase-1, geranylgeranyltransferase-I, geranylgeranyltransferaseI, GGT, GGT I, GGTalpha, GGTase, GGTase I, Ggtase-1, GGTase-I, GGTaseI, GGTbeta, GGTI, mammalian GGTase-I, PGGT, PGGT beta, PGGT I, PGGT-I, PGGTase-I, PGGTaseI, Ppggb, protein geranylgeranyltransferase, protein geranylgeranyltransferase I, protein geranylgeranyltransferase type I, protein geranylgeranyltransferase type-I, protein geranylgeranyltransferase-I, type I protein geranylgeranyltransferase
the cdc43DELTA mutant is created by replacing the entire open reading frame in H99 strain with the dominant nourseothricin (NAT) resistance gene. The cdc43DELTA mutant has a growth defect at 37°C and 39°C. The CDC43 locus is completely deleted in several isolates. The morphology of the cdc43dELTA mutant is indistinguishable from that of the wild-type when grown at 30°C. Both the temperature sensitivity and the morphological defects of the cdc43DELTA mutant are completely rescued by the reintroduction of the wild-type CDC43 allele in the cdc43DELTA CDC43 complemented strain
the cdc43DELTA mutant is created by replacing the entire open reading frame in H99 strain with the dominant nourseothricin (NAT) resistance gene. The cdc43DELTA mutant has a growth defect at 37°C and 39°C. The CDC43 locus is completely deleted in several isolates. The morphology of the cdc43dELTA mutant is indistinguishable from that of the wild-type when grown at 30°C. Both the temperature sensitivity and the morphological defects of the cdc43DELTA mutant are completely rescued by the reintroduction of the wild-type CDC43 allele in the cdc43DELTA CDC43 complemented strain
comparison of substrate specificity, using GST-CAIL and geranylgeranyl diphosphate: best substrate of wild-type enzyme, using GST-CVIM and geranylgeranyl diphosphate: best substrate of mutant beta H216D, using GST-CAIL and farnesyl diphosphate: best substrate of mutant betaR166I
cal1-1 mutation preferentially affects Rho1p geranylgeranylation, and it can be suppressed by Rho1p overexpression, the cdc43-5 mutation in the same gene causes accumulation of Cdc42p in soluble form and can be suppressed by Cdc42p overproduction
seven different mutations in CAL1/CDC43 gene: cal1-1 and cdc43-2 to cdc43-7, all of mutants possess reduced activity and none show temperature-sensitive enzymatic activities, but all of them show temperature-sensitive growth phenotypes
seven different mutations in CAL1/CDC43 gene: cal1-1 and cdc43-2 to cdc43-7, all of mutants possess reduced activity and none show temperature-sensitive enzymatic activities, but all of them show temperature-sensitive growth phenotypes
Cwg2-1 mutant has a defect in the actin organization, this mutant is identified as a single nucleotide change causing an A202T substitution in a residue conserved among the beta-subunit of enzyme, the deletion of cwg2 causes cell death