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2.5.1.56: N-acetylneuraminate synthase

This is an abbreviated version!
For detailed information about N-acetylneuraminate synthase, go to the full flat file.

Word Map on EC 2.5.1.56

Reaction

phosphoenolpyruvate
+
N-acetyl-D-mannosamine
 +
H2O
=
phosphate
 +
N-acetylneuraminate

Synonyms

(NANA) condensing enzyme, EC 4.1.3.19, N-acetylneuraminate lyase synthase, N-acetylneuraminate pyruvate-lyase (pyruvate-phosphorylating), N-acetylneuraminic acid condensing enzyme, N-acetylneuraminic acid synthase, NANA condensing enzyme, NANA synthase, NANAS, NANS, NeuAc synthase, NeuB, NeuB1, NeuNAc synthase, NmeNANAS, sialic acid synthase, synthase, N-acetylneuraminate

ECTree

     2 Transferases
         2.5 Transferring alkyl or aryl groups, other than methyl groups
             2.5.1 Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
                2.5.1.56 N-acetylneuraminate synthase

Cloned

Cloned on EC 2.5.1.56 - N-acetylneuraminate synthase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) Gold cells
-
expressed in Escherichia coli strain K12
expression in Escherichia coli (BL21 deltaDE3) with pCWori+ vector
expression of the neuB1 gene in Escherichia coli
-
gene MvNeuB, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of His6-tagged enzyme in Escherichia coli strain Rosetta 2 (DE3) pLysS
gene siaC, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain Rosetta 2, subcloningin Escherichia coli strain DH5alpha
overexpression in Escherichia coli
-