2.5.1.49: O-acetylhomoserine aminocarboxypropyltransferase

This is an abbreviated version!
For detailed information about O-acetylhomoserine aminocarboxypropyltransferase, go to the full flat file.

Reaction

Synonyms

AHS, EC 4.2.99.10, gamma-cyano-alpha-aminobutyric acid synthase, Homocysteine synthase, MetC, methionine synthase, MetY, O-acetyl-L-homoserine (thiol)-lyase, O-acetyl-L-homoserine acetate-lyase (adding methanethiol), O-acetyl-L-homoserine sulfhydrolase, o-acetyl-L-homoserine sulfhydrylase, O-acetylhomoserine sulfhydrolase, O-acetylhomoserine sulfhydrylase, OAH, OAH SHase, OAH SHLase, OAH sulfhydrylase, OAH-OAS sulfhydrylase, oah2 gene product, OAHS, OAS-OAH sulfhydrylase, Q187D4

ECTree

     2 Transferases

         2.5 Transferring alkyl or aryl groups, other than methyl groups

             2.5.1 Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)

                2.5.1.49 O-acetylhomoserine aminocarboxypropyltransferase

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Results	in table
5	Activating Compound
11400	AA Sequence
3	Application
1	CAS Registry Number
9	Cloned(Commentary)
15	Cofactor
2	Crystallization (Commentary)
2	Disease/ Diagnostics
1	General Information
6	General Stability
2	IC50 Value
74	Inhibitors
3	kcat/KM [mM/s]
17	Ki Value [mM]
41	KM Value [mM]
2	Metals/Ions
32	Molecular Weight [Da]
25	Natural Substrates/ Products (Substrates)
1	Organic Solvent Stability
47	Organism
7	Pathway
16	PDB ID
13	pH Optimum
4	pH Range
4	pH Stability
1	pI Value
18	Purification (Commentary)
1	Reaction
4	Reaction Type
43	Reference
16	Source Tissue
38	Specific Activity [micromol/min/mg]
6	Storage Stability
145	Substrates and Products (Substrate)
23	Subunits
51	Synonyms
1	Systematic Name
4	Temperature Optimum [°C]
1	Temperature Range [°C]
6	Temperature Stability [°C]
3	Turnover Number [1/s]

General Stability

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General Stability on EC 2.5.1.49 - O-acetylhomoserine aminocarboxypropyltransferase

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GENERAL STABILITY

ORGANISM

UNIPROT

LITERATURE

enzyme appears to be quite stable

Neurospora crassa

-

637823

enzyme remains stable after purification, no dilution inactivation is observed

Neurospora crassa

-

637824

existence of glycerol in the purification buffer keeps the enzyme very stable, sensitive to salts such as (NH4)2SO4 and NaCl in absence of glycerol

Aspergillus nidulans

-

637836

not stable even in presence of a cofactor

Corynebacterium acetophilum

-

637830

sensitive to trypsin digestion

Saccharomyces cerevisiae

-

637838

very unstable, can be stabilized by addition of 25% sucrose or glycerol

Schizosaccharomyces pombe

-

637834