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0.018 - 2.5
5-thio-2-nitrobenzoate
0.785 - 1.053
chloroalanine
0.7
dithiothreitol
pH 7.4, 37°C
0.06 - 6.4
hydrogen sulfide
0.25
L-cysteine
pH 7.5, 45°C
0.148 - 50
O-acetyl-L-Ser
0.037 - 15
O-acetyl-L-serine
6.67
O-acetylhomoserine
-
-
1.03 - 1.355
O-acetylserine
227
O-phosphoserine
-
37°C, pH 7.8
0.11 - 16
O3-acetyl-L-serine
0.133
sodium thiosulfate
-
-
additional information
additional information
-
0.018
5-thio-2-nitrobenzoate
-
mutant W50Y, pH 7.0, 20°C
0.049
5-thio-2-nitrobenzoate
-
wild-type, pH 7.0, 20°C
0.061
5-thio-2-nitrobenzoate
-
mutant W161Y, pH 7.0, 20°C
0.07
5-thio-2-nitrobenzoate
-
mutant H52A, pH 6.5
0.07
5-thio-2-nitrobenzoate
-
K120Q mutant with 9fold decrease compared to wild type, 100 mM MES, pH 6.5, 0.5 mM O3-acetyl-L-serine, 25°C
0.12
5-thio-2-nitrobenzoate
-
pH 7.0, 50°C
0.6
5-thio-2-nitrobenzoate
-
wild-type, pH 7.0
0.6
5-thio-2-nitrobenzoate
-
wild type, 100 mM HEPES, pH 7.0, 0.5 mM O3-acetyl-L-serine, 25°C
2.5
5-thio-2-nitrobenzoate
-
mutant H52A, pH 6.5
2.5
5-thio-2-nitrobenzoate
-
H152A mutant with 4.1fold increase compared to wild type, 100 mM MES, pH 6.5, 0.5 mM O3-acetyl-L-serine, 25°C
0.12
acetyl-CoA
-
-
0.785
chloroalanine
-
pH 8.0, 26°C, cysteine synthase B
1.053
chloroalanine
-
pH 8.0, 26°C, cysteine synthase A
14.2
CN-
-
pH 8.5, enzyme form PCS-1
15.1
CN-
-
pH 8.5, enzyme form PCS-2
0.0384
cysteine
-
pH 8.5, enzyme form PCS-2
0.0512
cysteine
-
pH 8.5, enzyme form PCS-1
0.0526
cysteine
-
pH 7.5, 37°C, isoenzyme C
0.0986
cysteine
-
isoenzyme A
0.108
cysteine
-
pH 8.0, 26°C, cysteine synthase A
0.113
cysteine
-
pH 7.5, 37°C, isoenzyme B
0.201
cysteine
-
pH 8.0, 26°C, cysteine synthase B
0.006
H2S
-
pH 7.5, 25°C, free enzyme
0.013
H2S
-
pH 7.5, 25°C, enzyme bound to serine acetyltransferase
0.029
H2S
-
37°C, pH 7.8, cosubstrate: O-phosphoserine
0.033
H2S
-
pH 8.0, 30°C, isoenzyme 1
0.038
H2S
-
pH 7.8, 35°C, isoenzyme 2
0.073
H2S
mutant Y97F, pH 8.0, 30°C
0.084
H2S
mutant Y97M, pH 8.0, 30°C
0.095
H2S
mutant S121A, pH 8.0, 30°C
0.12
H2S
wild-type, pH 8.0, 30°C
0.14
H2S
mutant V74T, pH 8.0, 30°C
0.16
H2S
-
at pH 7.4 and 25°C
0.2
H2S
mutant S121M, pH 8.0, 30°C
0.25
H2S
-
pH 7.5, 25°C, chloroplast enzyme
0.37
H2S
-
pH 7.0, 25°C, recombinant free enzyme
0.4
H2S
-
pH 7.4, 37°C, enzyme 1
0.55
H2S
-
pH 8.0, 25°C, isoenzyme 1
0.55
H2S
-
pH 7.5, 25°C, recombinant complex-bound enzyme
0.6
H2S
-
isoenzyme 1 and 2
0.66
H2S
-
pH 8.0, 25°, isoenzyme 1'
0.8
H2S
-
pH 7.5, 37°C, isoenzyme 1 and 2
0.8
H2S
-
37°C, pH 7.8, cosubstrate: O-acetyl-L-Ser
0.998
H2S
-
pH 7.5, enzyme form PCS-2
1.25
H2S
-
pH 8.0, 25°C, isoenzyme 2
1.57
H2S
-
pH 7.5, enzyme form PCS-1
1.6
H2S
-
pH 7.4, 37°C, isoenzyme 2
2.5
H2S
-
pH 8.0, 25°C, isoenzyme 3
5.2
H2S
-
pH 7.5, 50°C, isoenzyme 1 and 2
0.06
hydrogen sulfide
pH 7.0, 40°C
0.078
hydrogen sulfide
pH 7.5, 23°C
0.12
hydrogen sulfide
30°C, pH 8.0
0.252
hydrogen sulfide
pH 6.5, 50°C
1.46
hydrogen sulfide
pH 7.5, 22°C, recombinant enzyme, Lineweaver-Burke model
1.85
hydrogen sulfide
pH 7.5, 22°C, recombinant enzymem, Michaelis-Menten model
2.3
hydrogen sulfide
pH 7.5, 25°C
3
hydrogen sulfide
-
isoform B, pH 7.5, 25°C, Michaelis-Menten kinetics
3.2
hydrogen sulfide
-
isoform B, pH 7.5, 25°C, Hill equation, Hill coefficient 0.75
4.6
hydrogen sulfide
-
isoform C, pH 7.5, 25°C, Hill equation, Hill coefficient 1.05
4.7
hydrogen sulfide
-
isoform C, pH 7.5, 25°C, Michaelis-Menten kinetics
5.6
hydrogen sulfide
-
isoform A, pH 7.5, 25°C, Michaelis-Menten kinetics
6.4
hydrogen sulfide
-
isoform A, pH 7.5, 25°C, Hill equation, Hill coefficient 0.81
0.7
L-Cys
pH 7.4, 37°C
0.7
L-Ser
-
-
0.148
O-acetyl-L-Ser
pH 7.5, 23°C
0.159
O-acetyl-L-Ser
pH 6.5, 50°C
0.6
O-acetyl-L-Ser
pH 7.4, 37°C
0.64
O-acetyl-L-Ser
-
pH 7.5, 25°C, recombinant free enzyme
0.7
O-acetyl-L-Ser
-
pH 7.5, 37°C, isoenzyme B
0.7
O-acetyl-L-Ser
-
pH 7.5, 37°C, isoenzyme 1 and 2
0.838
O-acetyl-L-Ser
pH 7.0, 40°C
1.3
O-acetyl-L-Ser
-
pH 7.5, 25°C, chloroplast enzyme
1.5
O-acetyl-L-Ser
-
pH 8.0, 30°C, isoenzyme 2
2.1
O-acetyl-L-Ser
-
pH 8.0, 30°C, isoenzyme 1
2.1
O-acetyl-L-Ser
-
pH 7.5, 37°C, isoenzyme A
2.1
O-acetyl-L-Ser
-
formation of beta-(isoxazylin5-on-4-yl)-L-alanine
2.3
O-acetyl-L-Ser
-
pH 8.0, 30°C, isoenzyme 2
2.5
O-acetyl-L-Ser
-
pH 8.0, 30°C
2.6
O-acetyl-L-Ser
-
pH 8.0, 30°C, isoenzyme 1
2.7
O-acetyl-L-Ser
-
pH 7.5, 30°C
2.9
O-acetyl-L-Ser
-
isoenzyme 1
2.9
O-acetyl-L-Ser
-
pH 8.0, 30°C
3.1
O-acetyl-L-Ser
-
pH 7.8, 35°C
3.57
O-acetyl-L-Ser
-
pH 8.0, 25°C, isoenzyme 1
3.8
O-acetyl-L-Ser
pH 7.5, 25°C
3.8
O-acetyl-L-Ser
-
formation of beta-(isoxazylin5-on-2-yl)-L-alanine
3.9
O-acetyl-L-Ser
-
pH 7.5, 37°C, isoenzyme C
4.28
O-acetyl-L-Ser
-
pH 7.5, 25°C, recombinant complex-bound enzyme
4.8
O-acetyl-L-Ser
-
pH 7.5, 25°C, free enzyme
4.8
O-acetyl-L-Ser
-
pH 7.8, 50°C
5
O-acetyl-L-Ser
-
free enzyme
5
O-acetyl-L-Ser
-
pH 7.2-7.4, 25°C
5
O-acetyl-L-Ser
-
independent of sulfide concentration
5.12
O-acetyl-L-Ser
-
pH 7.8
5.26
O-acetyl-L-Ser
-
pH 8.0, 25°C, isoenzyme 3
5.56
O-acetyl-L-Ser
-
pH 8.0, 25°C, isoenzyme 2
6
O-acetyl-L-Ser
pH 7.5, 37°C
6.7 - 8.3
O-acetyl-L-Ser
-
pH 7.4, 30°C, cosubstrate-dependent
8.2
O-acetyl-L-Ser
-
pH 7.6, 25°C
8.3
O-acetyl-L-Ser
-
pH 8.0, 25°, isoenzyme 1'
9
O-acetyl-L-Ser
-
isoenzyme 2
15.86
O-acetyl-L-Ser
pH 7.5, 37°C
20
O-acetyl-L-Ser
-
complex-bound enzyme
24
O-acetyl-L-Ser
-
synthesis of S-sulfo-L-cysteine
27
O-acetyl-L-Ser
-
pH 7.5, 25°C, enzyme bound to serine acetyltransferase
28
O-acetyl-L-Ser
-
sulfhydrylation of O-acetyl-L-serine
39.5
O-acetyl-L-Ser
-
37°C, pH 7.8
50
O-acetyl-L-Ser
-
isoenzyme 2
0.037
O-acetyl-L-serine
-
pH 6.8, 37°C
0.045
O-acetyl-L-serine
Xanthium pennsylvanicum
-
,isoenzyme 3
0.05
O-acetyl-L-serine
Xanthium pennsylvanicum
-
isoenzymes 1 and 2
0.063
O-acetyl-L-serine
-
0.105
O-acetyl-L-serine
-
0.11
O-acetyl-L-serine
-
mutant H52A, pH 6.5
0.116
O-acetyl-L-serine
-
0.355
O-acetyl-L-serine
-
0.373
O-acetyl-L-serine
Xanthium pennsylvanicum
-
,isoenzyme 2
0.41
O-acetyl-L-serine
-
at pH 7.4 and 25°C
0.46
O-acetyl-L-serine
-
-
0.547
O-acetyl-L-serine
Xanthium pennsylvanicum
-
,isoenzyme 3
0.6
O-acetyl-L-serine
-
mutant T68S, 37°C
0.7
O-acetyl-L-serine
-
wild-type, 37°C
1
O-acetyl-L-serine
-
pH 7.4, 37°C, isoenzyme 1
1
O-acetyl-L-serine
-
mutant H52A, pH 6.5
1
O-acetyl-L-serine
recombinant enzyme, pH 7.0, 25°C, with DTT
1.1
O-acetyl-L-serine
recombinant enzyme, pH 7.0, 25°C, with DTT
1.168
O-acetyl-L-serine
A0A1J9VES8
wild-type, pH 8.2, 37°C
1.29
O-acetyl-L-serine
Xanthium pennsylvanicum
-
isoenzyme 1
1.3
O-acetyl-L-serine
-
pH 6.8, 36°C
1.65
O-acetyl-L-serine
A0A1J9VES8
mutant A72S, pH 8.2, 37°C
1.8
O-acetyl-L-serine
recombinant enzyme, pH 7.0, 25°C
1.83
O-acetyl-L-serine
-
pH 7.5, enzyme form PCS-2
2
O-acetyl-L-serine
-
pH 7.5, 50°C
2.05
O-acetyl-L-serine
A0A1J9VES8
mutant E220R, pH 8.2, 37°C
2.3
O-acetyl-L-serine
recombinant enzyme, pH 7.0, 25°C
2.99
O-acetyl-L-serine
-
pH 7.5, enzyme form PCS-1
3.5
O-acetyl-L-serine
-
pH 7.8, 35°C
3.5
O-acetyl-L-serine
recombinant enzyme, pH 7.0, 25°C, with glutathione, GSSG
4.8
O-acetyl-L-serine
recombinant enzyme, pH 7.0, 25°C, with glutathione, GSSG
6.18
O-acetyl-L-serine
A0A1J9VES8
production of H2S, wild-type, pH 8.2, 37°C
9.01
O-acetyl-L-serine
A0A1J9VES8
production of H2S, mutant A72S, pH 8.2, 37°C
9.6
O-acetyl-L-serine
-
pH 7.5, 50°C, isoenzyme 1 and 2
15
O-acetyl-L-serine
-
wild-type, pH 7.0
15
O-acetyl-L-serine
-
pH 7.4, 37°C, isoenzyme 2
1.03
O-acetylserine
-
pH 8.0, 26°C, cysteine synthase B
1.355
O-acetylserine
-
pH 8.0, 26°C, cysteine synthase A
0.11
O3-acetyl-L-serine
-
H152A mutant with 136fold decrease compared to wild type, 100 mM MES, pH 6.5, 50 microM 5-thio-2-nitrobenzoate (TNB), 25°C
0.31
O3-acetyl-L-serine
-
isoform B, pH 7.5, 25°C, Michaelis-Menten kinetics
0.32
O3-acetyl-L-serine
-
isoform B, pH 7.5, 25°C, Hill equation, Hill coefficient 1.03
0.36
O3-acetyl-L-serine
-
pH 7.0, 50°C
0.43
O3-acetyl-L-serine
-
isoform C, pH 7.5, 25°C, Michaelis-Menten kinetics
0.51
O3-acetyl-L-serine
-
isoform C, pH 7.5, 25°C, Hill equation, Hill coefficient 0.86
0.57
O3-acetyl-L-serine
-
mutant S269A, pH 7.0, 25°C
0.66
O3-acetyl-L-serine
-
isoform A, pH 7.5, 25°C, Hill equation, Hill coefficient 1.05
0.68
O3-acetyl-L-serine
-
mutant T78A, pH 7.0, 25°C
0.69
O3-acetyl-L-serine
-
isoform A, pH 7.5, 25°C, Michaelis-Menten kinetics
0.749
O3-acetyl-L-serine
-
wild-type, pH 7.0, 20°C
0.774
O3-acetyl-L-serine
-
mutant W50Y, pH 7.0, 20°C
0.82
O3-acetyl-L-serine
-
mutant T78S, pH 7.0, 25°C
0.91
O3-acetyl-L-serine
-
mutant H157Q, pH 7.0, 25°C
0.94
O3-acetyl-L-serine
-
mutant H157N, pH 7.0, 25°C
0.96
O3-acetyl-L-serine
-
mutant S269T, pH 7.0, 25°C
1
O3-acetyl-L-serine
-
K120Q mutant with 15fold decrease compared to wild type, 100 mM MES, pH 6.5, 50 microM 5-thio-2-nitrobenzoate (TNB), 25°C
1.2
O3-acetyl-L-serine
-
mutant S75T, pH 7.0, 25°C
1.2
O3-acetyl-L-serine
-
mutant T182A, pH 7.0, 25°C
1.4
O3-acetyl-L-serine
-
wild-type, pH 7.0, 25°C
1.5
O3-acetyl-L-serine
-
mutant T185A, pH 7.0, 25°C
1.5
O3-acetyl-L-serine
-
mutant T74S, pH 7.0, 25°C
1.6
O3-acetyl-L-serine
-
mutant S75A, pH 7.0, 25°C
1.6
O3-acetyl-L-serine
-
mutant T182S, pH 7.0, 25°C
1.6
O3-acetyl-L-serine
-
mutant T185S, pH 7.0, 25°C
1.6
O3-acetyl-L-serine
-
mutant T74A, pH 7.0, 25°C
1.771
O3-acetyl-L-serine
-
mutant W161Y, pH 7.0, 20°C
2
O3-acetyl-L-serine
-
mutant S75N, pH 7.0, 25°C
3.8
O3-acetyl-L-serine
-
mutant Q147S, pH 7.0, 25°C
4.4
O3-acetyl-L-serine
-
mutant Q147A, pH 7.0, 25°C
4.7
O3-acetyl-L-serine
-
mutant N77A, pH 7.0, 25°C
7
O3-acetyl-L-serine
-
37°C, pH 9.0
10.2
O3-acetyl-L-serine
-
mutant N77D, pH 7.0, 25°C
15
O3-acetyl-L-serine
-
wild type, 100 mM HEPES, pH 7.0, 50 microM 5-thio-2-nitrobenzoate (TNB), 25°C
16
O3-acetyl-L-serine
pH 7.5, 45°C
0.031
Sulfide
-
37°C, pH 9.0
0.18
Sulfide
-
mutant N77D, pH 7.0, 25°C
0.21
Sulfide
-
mutant S75T, pH 7.0, 25°C
0.22
Sulfide
-
wild-type, pH 7.0, 25°C
0.22
Sulfide
-
mutant T182S, pH 7.0, 25°C
0.22
Sulfide
-
mutant T185A, pH 7.0, 25°C
0.23
Sulfide
-
mutant N77A, pH 7.0, 25°C
0.27
Sulfide
-
mutant Q147S, pH 7.0, 25°C
0.3
Sulfide
-
mutant H157Q, pH 7.0, 25°C
0.3
Sulfide
-
mutant T78S, pH 7.0, 25°C
0.36
Sulfide
-
mutant T185S, pH 7.0, 25°C
0.39
Sulfide
-
mutant T182A, pH 7.0, 25°C
0.4
Sulfide
-
mutant H157N, pH 7.0, 25°C
0.44
Sulfide
-
mutant T78A, pH 7.0, 25°C
0.54
Sulfide
-
mutant S269A, pH 7.0, 25°C
1.6
Sulfide
-
mutant S269T, pH 7.0, 25°C
1.7
Sulfide
-
mutant Q147A, pH 7.0, 25°C
3.2
Sulfide
-
mutant T74S, pH 7.0, 25°C
3.9
Sulfide
-
mutant T74A, pH 7.0, 25°C
4.6
Sulfide
-
mutant S75A, pH 7.0, 25°C
5.7
Sulfide
-
mutant S75N, pH 7.0, 25°C
0.93
thiosulfate
-
-
21
thiosulfate
-
synthesis of S-sulfo-L-cysteine
22
thiosulfate
30°C, pH 8.0
additional information
additional information
-
-
-
additional information
additional information
-
Hill numbers
-
additional information
additional information
-
cysteine-forming activity 245 times greater than beta-cyanoalanine-forming activity
-
additional information
additional information
-
no Michaelis-Menten-kinetics
-
additional information
additional information
-
positive kinetic cooperativity with respect to O-acetylserine in the presence of sulfide
-
additional information
additional information
-
activity varies between sulfur sources
-
additional information
additional information
-
not significantly altered by immobilization
-
additional information
additional information
-
various methods compared
-
additional information
additional information
-
various methods compared
-
additional information
additional information
-
HPLC method for product quantification
-
additional information
additional information
the B-isozyme has a an about 10fold lower Km for O-acetyl-L-serine than the A-isoenzyme
-
additional information
additional information
the B-isozyme has a an about 10fold lower Km for O-acetyl-L-serine than the A-isoenzyme
-
additional information
additional information
-
the B-isozyme has a an about 10fold lower Km for O-acetyl-L-serine than the A-isoenzyme
-
additional information
additional information
important role of the C-terminal residue Ile5 and the arylic moiety at P3 in dictating enzyme affinity, dissociation constant of MNYDI
-
additional information
additional information
important role of the C-terminal residue Ile5 and the arylic moiety at P3 in dictating enzyme affinity, dissociation constant of MNYDI
-
additional information
additional information
-
important role of the C-terminal residue Ile5 and the arylic moiety at P3 in dictating enzyme affinity, dissociation constant of MNYDI
-
additional information
additional information
important role of the C-terminal residue Ile5 and the arylic moiety at P3 in dictating enzyme affinity, dissociation constant of MNYDI
-
additional information
additional information
steady-state kinetics and modeling
-
additional information
additional information
-
steady-state kinetics and modeling
-