2.5.1.46: deoxyhypusine synthase
This is an abbreviated version!
For detailed information about deoxyhypusine synthase, go to the full flat file.
Word Map on EC 2.5.1.46
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2.5.1.46
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polyamine
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gc7
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homospermidine
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pyrrolizidine
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n1-guanyl-1,7-diaminoheptane
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nepsilon-4-amino-2-hydroxybutyllysine
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aminobutylation
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hypusine-containing
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1,7-diaminoheptane
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deoxyhypusine-containing
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butylamine
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polyamine-derived
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eif-4d
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medicine
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1,3-diaminopropane
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triamine
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wolff
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drug development
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analysis
- 2.5.1.46
- polyamine
- gc7
- homospermidine
-
pyrrolizidine
- n1-guanyl-1,7-diaminoheptane
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nepsilon-4-amino-2-hydroxybutyllysine
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aminobutylation
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hypusine-containing
- 1,7-diaminoheptane
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deoxyhypusine-containing
- butylamine
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polyamine-derived
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eif-4d
- medicine
- 1,3-diaminopropane
- triamine
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wolff
- drug development
- analysis
Reaction
Synonyms
CpDHS, deoxyhypusine synthase, deoxyhypusine synthase (Caulobacter crescentus gene CC0359), deoxyhypusine synthase (Halobacterium strain NRC-1 gene dhs), deoxyhypusine synthase (human clone 30649 gene DHPS subunit reduced), deoxyhypusine synthase (Nicotiana tabacum gene DHS1), deoxyhypusine synthase (Senecio vernalis gene DHS1), deoxyhypusinesynthase, DHPS, DHS, DHS1, DHS2, DHS20, DHS34, DHSc, DHSp, DHYS, Dys1, Dys1p, EC 1.1.1.249, HVO 2297, PF3D7_1412600, speY, synthase, deoxyhypusine, TbDHSc, TbDHSp
ECTree
2.5.1.46 deoxyhypusine synthaseAdvanced search results
results (
results (Engineering
Engineering on EC 2.5.1.46 - deoxyhypusine synthase
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PROTEIN VARIANTS 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
H288A
N173S
recurrent missense variant identified with likely gene disrupting variant (c.1014+1G>A, c.912_917delTTACAT [p.Tyr305_Ile306del]), or c.1A>G [p.Met1] in unrelated individuals having similar neurodevelopmental features that include global developmental delay and seizures. Two of four affected females have short stature. The c.1014+1G>A variant causes aberrant splicing. Recombinant p.Asn173Ser or p.Tyr305_Ile306del protein show reduced (20%) or absent in vitro activity, respectively. The p.Tyr305_Ile306del and p.Asn173Ser variants result in reduced hypusination of eIF5A compared to wild-type DHPS enzyme
L344K
mutant enzyme is still devoid of any DHS activity, suggesting that other differences in amino acid sequences also contribute to its inactivity
D314A
site-directed mutagenesis, the mutant is active as deoxyhypusine synthase, but inactive as deoxyhypusine hydroxylase
H313A
site-directed mutagenesis, the mutant is active as deoxyhypusine synthase, but inactive as deoxyhypusine hydroxylase
D314A
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site-directed mutagenesis, the mutant is active as deoxyhypusine synthase, but inactive as deoxyhypusine hydroxylase
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H313A
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site-directed mutagenesis, the mutant is active as deoxyhypusine synthase, but inactive as deoxyhypusine hydroxylase
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D218A
mutation in catalytic site of subunit DHSp, mutant is unable to catalyze the single-turnover formation of NADH
D405A
mutation in catalytic site of subunit DHSc, impaired in activity
E103A
mutation in catalytic site of subunit DHSp, impaired in activity
E104A
mutation in catalytic site of subunit DHSp, impaired in activity
E166A
mutation in dead site of subunit DHSc, activity is similar to wild-type. The heterotetrameric complex shows a decrease in melting temperature by 4.9 degrees
H266A
mutation in dead site of subunit DHSp, activity is similar to wild-type
D218A
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mutation in catalytic site of subunit DHSp, mutant is unable to catalyze the single-turnover formation of NADH
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D405A
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mutation in catalytic site of subunit DHSc, impaired in activity
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E103A
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mutation in catalytic site of subunit DHSp, impaired in activity
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H266A
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mutation in dead site of subunit DHSp, activity is similar to wild-type
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additional information
additional information
generation of deoxyhypusine synthase knockout mutants using siRNA via Agrobacterium strainGV3101/pMP90 transfection in Arabidopsis thaliana
additional information
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generation of deoxyhypusine synthase knockout mutants using siRNA via Agrobacterium strainGV3101/pMP90 transfection in Arabidopsis thaliana
additional information
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generation of deoxyhypusine synthase knockout mutants using siRNA via Agrobacterium strainGV3101/pMP90 transfection in Arabidopsis thaliana
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additional information
replacement of the endogenous DHS and DOHH genes by homologous recombination with the PgpdA-DHS or Pgpd1-DOHH alleles, excluding any possibility of mutagenic effects by ectopic integration
additional information
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replacement of the endogenous DHS and DOHH genes by homologous recombination with the PgpdA-DHS or Pgpd1-DOHH alleles, excluding any possibility of mutagenic effects by ectopic integration
additional information
variant recombinant proteins with i. a truncation of 48 or 97 NH2-terminal amino acids, ii. A truncation of 39 COOH-terminal amino acids, or iii. An internal deletion (ASp262-Ser317) are inactive. A chimeric protein consisting of the complete human sequence and 16 amino acids of the yeast sequence, Gln197-Asn212, not present in the human enzyme, inserted between Glu193 and Gln194 exhibit moderate activity
additional information
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variant recombinant proteins with i. a truncation of 48 or 97 NH2-terminal amino acids, ii. A truncation of 39 COOH-terminal amino acids, or iii. An internal deletion (ASp262-Ser317) are inactive. A chimeric protein consisting of the complete human sequence and 16 amino acids of the yeast sequence, Gln197-Asn212, not present in the human enzyme, inserted between Glu193 and Gln194 exhibit moderate activity
additional information
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DHS knock-down by stable lentiviral expression of shRNAs in G55T2 and U87-MG cells
additional information
DHPS gene silencing by transfection of DHPS-specific short hairpin RNA (shRNA) in LM-pGL4.5 cells
additional information
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DHPS gene silencing by transfection of DHPS-specific short hairpin RNA (shRNA) in LM-pGL4.5 cells
additional information
during purification and crystallization, residue C177 is is covalently modified to a 2-mercaptoethanol adduct
additional information
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during purification and crystallization, residue C177 is is covalently modified to a 2-mercaptoethanol adduct
additional information
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DHS34 gene deletion mutations via targeted gene replacement, chromosomal null mutants of DHS34 are only obtained in the presence of a DHS34-containing episome. HYGDELTAdhs34 and PHLEODELLTAdhs34 gene replacement cassettes are individually transfected into wild-type parasites, phenotypes, verview
additional information
DHS34 gene deletion mutations via targeted gene replacement, chromosomal null mutants of DHS34 are only obtained in the presence of a DHS34-containing episome. HYGDELTAdhs34 and PHLEODELLTAdhs34 gene replacement cassettes are individually transfected into wild-type parasites, phenotypes, verview
additional information
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DHS34 gene deletion mutations via targeted gene replacement, chromosomal null mutants of DHS34 are only obtained in the presence of a DHS34-containing episome. HYGDELTAdhs34 and PHLEODELLTAdhs34 gene replacement cassettes are individually transfected into wild-type parasites, phenotypes, verview
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additional information
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construction of heterozygous or homozygous knockout mice via stem cell lines, phenotypes, overview
additional information
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construction of heterozygous or homozygous knockout mice via stem cell lines, phenotypes, overview
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additional information
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generation of the dys1-1 mutant that has a defect in total protein synthesis, a defect in polysome profile indicative of a translation elongation defect and a reduced association of eIF5A with polysomes
additional information
generation of a knockout mutant by gene replacement
additional information
generation of a knockout mutant by gene replacement
additional information
mutation of sets of matched pairs, where the same residue is changed in both the catalytic and dead site, melting temperature of mutants is similar to wild-type
additional information
Trypanosoma brucei brucei 927 / 4 GUTat10.1 / TREU927
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generation of a knockout mutant by gene replacement
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additional information
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mutation of sets of matched pairs, where the same residue is changed in both the catalytic and dead site, melting temperature of mutants is similar to wild-type
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