2.5.1.43: nicotianamine synthase

This is an abbreviated version!
For detailed information about nicotianamine synthase, go to the full flat file.

Reaction

3 S-adenosyl-L-methionine = 3 S-methyl-5'-thioadenosine +

Synonyms

AtNAS, AtNAS1, AtNAS2, AtNAS3, AtNAS4, chloronerva, HvNAS1, HvNAS2, HvNAS3, HvNAS4, HvNAS6, HvNAS7, HvNAS8, HvNAS9, LeNAS, LjNAS1, LjNAS2, MtNAS, MxNAS2, NA synthase, NA synthase 2, NAS, NAS1, NAS2, NAS3, NAS4, NASHOR1, NASHOR2, nicotianamine synthase, nicotianamine synthase 1, nicotianamine synthase 2, nicotianamine synthase 4, OsNA S2, OsNAS1, OsNAS2, OsNAS3, S-adenosyl-L-methionine:S-adenosyl-L-methionine:S-adenosyl-methionine 3-amino-3-carboxypropyltransferase, synthase, nicotianamine, TaNAS1, TaNAS1-A, TaNAS1-B, TaNAS2, TaNAS2-A, TaNAS2-D1, TaNAS2-D2, TaNAS3-A, TaNAS3-B, TaNAS4-A, TaNAS4-D, TaNAS4-U, TaNAS5-B, TaNAS6-A, TaNAS6-B, TaNAS6-D, TaNAS7-A1, TaNAS7-A2, TaNAS7-D, TaNAS9-A, TaNAS9-B, TaNAS9-D, TmNAS, TmNAS1, TmNAS2, TmNAS3, TmNAS4, TmNAS5, TmNAS6, TmNAS7, TmNAS8, ZmNAS1, ZmNAS10, ZmNAS1;1, ZmNAS1;2, ZmNAS2, ZmNAS2;1, ZmNAS2;2, ZmNAS3, ZmNAS4, ZmNAS5, ZmNAS6, ZmNAS6;1, ZmNAS6;2, ZmNAS7, ZmNAS8, ZmNAS9

ECTree

     2 Transferases

         2.5 Transferring alkyl or aryl groups, other than methyl groups

             2.5.1 Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)

                2.5.1.43 nicotianamine synthase

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Results	in table
1147	AA Sequence
6	Application
1	CAS Registry Number
47	Cloned(Commentary)
5	Cofactor
3	Crystallization (Commentary)
53	Expression
48	General Information
1	General Stability
10	Localization
13	Molecular Weight [Da]
29	Natural Substrates/ Products (Substrates)
131	Organism
4	Pathway
1	pH Optimum
1	pH Range
3	pH Stability
9	pI Value
3	Posttranslational Modification
13	Protein Variants
4	Purification (Commentary)
2	Reaction
1	Reaction Type
63	Reference
97	Source Tissue
1	Specific Activity [micromol/min/mg]
1	Storage Stability
58	Substrates and Products (Substrate)
24	Subunits
236	Synonyms
1	Systematic Name

Engineering

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Engineering on EC 2.5.1.43 - nicotianamine synthase

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PROTEIN VARIANTS

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

E81Q

2 entries

E81Q/Y107F

Methanothermobacter thermautotrophicus

-

inactive mutant

706512

L115A/L116A

Oryza sativa

Q10MI9

isozyme OsNAS2 mutated in the LL motif resulting in mutant m7-sGFP, which does not localize to the vesicles. Mutant m7–sGFP does not show NAS enzyme activity

739307

Y105A

Oryza sativa

Q10MI9

isozyme OsNAS2 mutated in the YXXphi motif resulting in mutant m6-sGFP, which is localized to the vesicles. These vesicles stuck together and are immobile. Mutant m6-sGFP converts S-adenosyl methionine into nicotinamine in vitro

739307

additional information

11 entries

E81Q

Methanothermobacter thermautotrophicus

-

site-directed mutagenesis, inactive mutant enzyme

722028

E81Q

Methanothermobacter thermautotrophicus

-

mutant binds the substrate but not the product

706512

additional information

Arabidopsis thaliana

-

breeding nas T-DNA insertion mutants leads to mutants without NAS activity (named nas4x-2, sterile, strongly chlorotic) and with reduced NAS activity especially during the reproductive phase (named nas4x-1, leaves, roots, and flowers with symptoms of iron deficiency)

706307

additional information

Arabidopsis thaliana

Q6L5P3

generation of a mutant Salk 135507 carrying a T-DNA insertion in gene AtNAS4

739365

additional information

Arabidopsis thaliana

-

generation of a mutant Salk 135507 carrying a T-DNA insertion in gene AtNAS4

739365

additional information

Arabidopsis thaliana Col-0

-

generation of a mutant Salk 135507 carrying a T-DNA insertion in gene AtNAS4

-

additional information

Oryza sativa

-

Oryza sativa OSNAS3 knockout plants and mutant lines are created in which expression of OsNAS3 gene is increased by introducing 35S enhancer elements. Shoots and roots of the OsNAS3 activation-tagged plants (OsNAS3-D1) accumulated more Fe and Zn, seeds contained elevated amounts of Fe (2.9-fold), Zn (2.2-fold), and Cu (1.7-fold).

706529

additional information

Oryza sativa

-

enhanced expression of OsNAS2 gene results in elevated nicotianamide levels, greater Zn accumulations, and improved plant tolerance to a Zn deficiency, the expression of Zn-uptake genes and those for the biosynthesis of phytosiderophores are increased in transgenic plants, phenotypes overview. In the endosperm, the OsNAS2 activation-tagged line contain up to 20fold more nicotianamide and 2.7fold more zinc

723378

additional information

Oryza sativa

-

generation of transgenic Oryza sativa plants by expression of iron-inducible nicotianamine synthase for increasing the content of bioavailable iron in seeds. Enhanced expression leads to higher tolerance of Fe deficiency and better growth under elevated pH, the iron content is increased 3fold by activating the recombinant NAS, phenotypes, overview

723191

additional information

Oryza sativa

Q10MI9

isozyme OsNAS2 mutated in the YXXphi motif, mutant m6-sGFP, is localized to the vesicles. These vesicles stuck together and are immobile. OsNAS2 mutated in the LL motif, mutant m7-sGFP, does not localize to these vesicles

739374

additional information

Oryza sativa

-

isozyme OsNAS2 mutated in the YXXphi motif, mutant m6-sGFP, is localized to the vesicles. These vesicles stuck together and are immobile. OsNAS2 mutated in the LL motif, mutant m7-sGFP, does not localize to these vesicles

739374

additional information

Oryza sativa

Q10MI9

mutant enzyme phenotype under both Fe-sufficient and -deficient conditions, overview

739307

additional information

Oryza sativa

-

mutant enzyme phenotype under both Fe-sufficient and -deficient conditions, overview

739307