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2.5.1.43: nicotianamine synthase

This is an abbreviated version!
For detailed information about nicotianamine synthase, go to the full flat file.

Word Map on EC 2.5.1.43

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3 S-adenosyl-L-methionine = 3 S-methyl-5'-thioadenosine +

nicotianamine

Synonyms

AtNAS, AtNAS1, AtNAS2, AtNAS3, AtNAS4, chloronerva, HvNAS1, HvNAS2, HvNAS3, HvNAS4, HvNAS6, HvNAS7, HvNAS8, HvNAS9, LeNAS, LjNAS1, LjNAS2, MtNAS, MxNAS2, NA synthase, NA synthase 2, NAS, NAS1, NAS2, NAS3, NAS4, NASHOR1, NASHOR2, nicotianamine synthase, nicotianamine synthase 1, nicotianamine synthase 2, nicotianamine synthase 4, OsNA S2, OsNAS1, OsNAS2, OsNAS3, S-adenosyl-L-methionine:S-adenosyl-L-methionine:S-adenosyl-methionine 3-amino-3-carboxypropyltransferase, synthase, nicotianamine, TaNAS1, TaNAS1-A, TaNAS1-B, TaNAS2, TaNAS2-A, TaNAS2-D1, TaNAS2-D2, TaNAS3-A, TaNAS3-B, TaNAS4-A, TaNAS4-D, TaNAS4-U, TaNAS5-B, TaNAS6-A, TaNAS6-B, TaNAS6-D, TaNAS7-A1, TaNAS7-A2, TaNAS7-D, TaNAS9-A, TaNAS9-B, TaNAS9-D, TmNAS, TmNAS1, TmNAS2, TmNAS3, TmNAS4, TmNAS5, TmNAS6, TmNAS7, TmNAS8, ZmNAS1, ZmNAS10, ZmNAS1;1, ZmNAS1;2, ZmNAS2, ZmNAS2;1, ZmNAS2;2, ZmNAS3, ZmNAS4, ZmNAS5, ZmNAS6, ZmNAS6;1, ZmNAS6;2, ZmNAS7, ZmNAS8, ZmNAS9

ECTree

     2 Transferases
         2.5 Transferring alkyl or aryl groups, other than methyl groups
             2.5.1 Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
                2.5.1.43 nicotianamine synthase

Cloned

Cloned on EC 2.5.1.43 - nicotianamine synthase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
analysis of upstream region of nicotianamide synthase gene from Arabidopsis thaliana: presence of putative ERE-like sequence
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complementation of the Lycopersicon esculentum mutant chloronerva that is free of nicotinamine due to a defect in nicotinamide synthase
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expressed in Lolium perenne after particle bombardment transformation
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expressed in Nicotiana tabacum
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expressed in Nicotiana tabacum, expression is highly induced by Fe-deficiency in roots
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expressed in Nicotiana tabacum, expression is highly induced by Fe-deficiency in roots and in leaves
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expressed in Oryza sativa cv. Tsukinohikari under control of the pGluB-1 promoter after transformation with Agrobacterium tumefaciens
expressed in Oryza sativa ssp. japonica cv. Taipei 309 under control of the 35S promoter from cauliflower mosaic virus after transformation by Agrobacterium tumefaciens
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expressed in Saccharomyces cerevisiae
expressed in Schizosaccharomyces pombe zhf cells
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expression in Escherichia coli
expression in rice. Overexpression of the nicotianamine synthase gene enhances nicotianamine level, enhances the Fe and Zn concentrations and confers tolerance to Fe deficiency in calcareous soil
expression in soybean, sweet potato, tobacco or rice. Overexpression of the nicotianamine synthase gene enhances nicotianamine level, enhances the Fe and Zn concentrations and confers tolerance to Fe deficiency in calcareous soil
expression of a barley HvNAS1 nicotinamine synthase gene promoter-gus fusion gene in transgenic tobacco is induced by Fe-deficiency in root
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expression of nicotianamine synthase in Oryza sativa seeds under the control of the maize ubiquitin promoter
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gene AtNAS4, overexpression of AtNAS4 both in the Salk 135507 mutant and the wild-type genetic backgrounds
gene MxNAS2, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, real-time PCR expression analysis, recombinant expression in transgenic Nicotiana tabacum cv. Xanthi under control of the CaMV 35S promoter, recombinant isozyme MxNAS2 promotes the synthesis of NAS and increases nicotinamine and chlorophyll contents in the tobacco cells. Overexpression of MxNAS2 improves the tolerance to Fe stress in transgenic tobacco, but leads to delayed flowering. Higher levels of MxNAS2 expression in transgenic tobacco contribute to misshapen flowers and increased levels of Fe, Mn, Cu and Zn in leaf and flower. A MxNAS2-GFP fusion protein is targeted into vesicles and cytoplasmic membrane
gene OsNAS2, overexpresion in Oryza sativa plants
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gene OsNAS2, recombinant expression of GFP-tagged isozyme NAS2 in transgenic Oryza sativa plants under the control of its own promoter. The recombinant GFP-tagged enzyme moves moving dynamically within root cells, phhenotype, ooverview
gene ZmNAS1, promoter identification, genotyping and isozyme sequence comparisons, phylogenetic analysis, quantitative RT-PCR expression analysis
gene ZmNAS10, promoter identification, genotyping and isozyme sequence comparisons, phylogenetic analysis, quantitative RT-PCR expression analysis
gene ZmNAS1;1, located on chromosome 9, genotying and phylogenetic analysis, sequence comparisons, expression analysis
gene ZmNAS1;2, located on chromosome 9, genotying and phylogenetic analysis, sequence comparisons, expression analysis
gene ZmNAS2, promoter identification, genotyping and isozyme sequence comparisons, phylogenetic analysis, quantitative RT-PCR expression analysis
gene ZmNAS2;1, located on chromosome 1, genotying and phylogenetic analysis, sequence comparisons, expression analysis
gene ZmNAS2;2, located on chromosome 1, genotying and phylogenetic analysis, sequence comparisons, expression analysis
gene ZmNAS3, located on chromosome 1, genotying and phylogenetic analysis, sequence comparisons, expression analysis
gene ZmNAS3, promoter identification, genotyping and isozyme sequence comparisons, phylogenetic analysis, quantitative RT-PCR expression analysis, recombinant expression of C-terminally GFP-tagged ZmNAS3 in the cytoplasm of transgenic Arabidopsis leaf protoplasts
gene ZmNAS4, located on chromosome 5, genotying and phylogenetic analysis, sequence comparisons, expression analysis
gene ZmNAS4, promoter identification, genotyping and isozyme sequence comparisons, phylogenetic analysis, quantitative RT-PCR expression analysis
gene ZmNAS5, located on chromosome 7, genotying and phylogenetic analysis, sequence comparisons, expression analysis
gene ZmNAS5, promoter identification, genotyping and isozyme sequence comparisons, phylogenetic analysis, quantitative RT-PCR expression analysis
gene ZmNAS6, promoter identification, genotyping and isozyme sequence comparisons, phylogenetic analysis, quantitative RT-PCR expression analysis
gene ZmNAS6;1, located on chromosome 9, genotying and phylogenetic analysis, sequence comparisons, expression analysis
gene ZmNAS6;2, located on chromosome 9, genotying and phylogenetic analysis, sequence comparisons, expression analysis
gene ZmNAS7, promoter identification, genotyping and isozyme sequence comparisons, phylogenetic analysis, quantitative RT-PCR expression analysis
gene ZmNAS8, promoter identification, genotyping and isozyme sequence comparisons, phylogenetic analysis, quantitative RT-PCR expression analysis
gene ZmNAS9, promoter identification, genotyping and isozyme sequence comparisons, phylogenetic analysis, quantitative RT-PCR expression analysis
overexpressed as a C-terminal His tag protein in Escherichia coli BL21 (DE3)
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recombinant expression of GFP-tagged isozyme OsNAS2 in Oryza sativa roots. OsNAS2-sGFP vesicles move dynamically in the cells. Fe homeostasis is disturbed in the GFP-tagged OsNAS2 plants, and these plants receive Fe-deficiency signals even under Fe-sufficient conditions, this is probably due to to the overproduction of deocxymugineic acid and nicotinamine, which increases the chelating capacity of Fe and disrupts an unknown Fe-sensing mechanism
sweet potato plants (Ipomoea batatas) expressing the nicotianamine synthase 1 (HvNAS1) gene under the control of CaMV 35S promoter are produced by Agrobacterium-mediated transformation
three cDNA clones osnas1, asnas2 and osnas3 from Fe-deficient rotts and a genomic fragment containing both OsNAS1 and OsNAS2
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three nicotianamine synthase genes: ZmNAS1, ZmNAS2 and ZmNAS3, fusion to the maltose-binding protein and production of the resulting fusion protein in Escherichia coli, ZmNAS1 and ZmNAS3 show nicotianamine synthase activity, ZmNAs2 does not
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transgenic Arabidopsis and tobacco plants constitutively overexpress the enzyme from Hordeum vulgare. Nicotianamine synthase overexpression results in increased biosynthesis of nicotianamine in transgenic plants, which conferrs enhanced tolerance of high levels of metals, particularly nickel, to plants. Promoter activities of four nicotianamine synthase genes in Arabidopsis are all increased in response to excess nickel, suggesting that nicotianamine plays an important role in the detoxification of nickel in plants. Transgenic tobacco plants with a high level of nicotianamine grew well in a nickel-enriched serpentine soil without developing any symptoms of nickel toxicity
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