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2.5.1.32: 15-cis-phytoene synthase

This is an abbreviated version!
For detailed information about 15-cis-phytoene synthase, go to the full flat file.

Word Map on EC 2.5.1.32

Reaction

2 geranylgeranyl diphosphate =

15-cis-phytoene
+ 2 diphosphate

Synonyms

AtPSY, CitPsy, CrtB, crtYB, EgcrtB, fruit-specific phytoene synthase, geranylgeranyl-diphosphate geranylgeranyltransferase, MaPsy1, MaPsy2, phytoene synthase, phytoene synthase 1, phytoene synthase 2, phytoene synthase/lycopene cyclase, phytoene synthetase, phytoene-synthetase, prephytoene-diphosphate synthase, Psase, PSY, PSY protein, PSY1, PSY1a, PSY1b, PSY1C, PSY2, PSY3, PSY5, synthetase, phytoene, sZmPSY1, TaPSY1, TaPSY2, TaPSY3, YELLOW-FRUITED TOMATO 2, yft2, Zmpsy1

ECTree

     2 Transferases
         2.5 Transferring alkyl or aryl groups, other than methyl groups
             2.5.1 Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
                2.5.1.32 15-cis-phytoene synthase

Purification

Purification on EC 2.5.1.32 - 15-cis-phytoene synthase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Sephadex G-25 gel filtration and ammonium sulfate fractionation
the purification protocol presents detailed instructions how to purify several milligrams of active enzyme from bacterial lysates, which includes initial recombinant enzyme enrichment through inclusion body purification, chaotropic unfolding, refolding in presence of detergents and purification through immobilized metal affinity chromatography
to facilitate purification of the recombinant enzyme, the structural gene for Psase is modified by site-directed mutagenesis to incorporate a C-terminal Glu-Glu-Phe tripeptide to allow purification by immunoaffinity chromatography on an immobilized monoclonal anti-alpha-tubulin antibody YL1/2 column
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