2.5.1.32: 15-cis-phytoene synthase
This is an abbreviated version!
For detailed information about 15-cis-phytoene synthase, go to the full flat file.
Word Map on EC 2.5.1.32
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2.5.1.32
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carotenoid
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desaturase
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carotenogenic
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ripening
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carotenogenesis
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orange
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zeaxanthin
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carotene
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astaxanthin
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lutein
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capsicum
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xanthophyl
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annuum
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xanthophyllomyces
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provitamin
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apocarotenoids
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dendrorhous
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violaxanthin
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zeta-carotene
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chromoplast
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nutrition
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alpha-carotene
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ananatis
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pantoea
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krabbe
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canthaxanthin
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analysis
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phaffia
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dunaliella
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9-cis-epoxycarotenoid
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ketolase
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uredovora
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pluvialis
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food industry
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ketocarotenoids
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norflurazon
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beta-cryptoxanthin
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beta-cyclase
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carra
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haematococcus
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biofortification
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biotechnology
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agriculture
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galcs
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rhodozyma
- 2.5.1.32
-
carotenoid
-
desaturase
-
carotenogenic
-
ripening
-
carotenogenesis
- orange
- zeaxanthin
- carotene
- astaxanthin
- lutein
- capsicum
-
xanthophyl
- annuum
- xanthophyllomyces
-
provitamin
-
apocarotenoids
- dendrorhous
- violaxanthin
- zeta-carotene
- chromoplast
- nutrition
- alpha-carotene
- ananatis
- pantoea
- krabbe
- canthaxanthin
- analysis
- phaffia
- dunaliella
-
9-cis-epoxycarotenoid
-
ketolase
- uredovora
- pluvialis
- food industry
-
ketocarotenoids
- norflurazon
- beta-cryptoxanthin
-
beta-cyclase
-
carra
- haematococcus
-
biofortification
- biotechnology
- agriculture
-
galcs
- rhodozyma
Reaction
2 geranylgeranyl diphosphate
=
Synonyms
AtPSY, CitPsy, CrtB, crtYB, EgcrtB, fruit-specific phytoene synthase, geranylgeranyl-diphosphate geranylgeranyltransferase, MaPsy1, MaPsy2, phytoene synthase, phytoene synthase 1, phytoene synthase 2, phytoene synthase/lycopene cyclase, phytoene synthetase, phytoene-synthetase, prephytoene-diphosphate synthase, Psase, PSY, PSY protein, PSY1, PSY1a, PSY1b, PSY1C, PSY2, PSY3, PSY5, synthetase, phytoene, sZmPSY1, TaPSY1, TaPSY2, TaPSY3, YELLOW-FRUITED TOMATO 2, yft2, Zmpsy1
ECTree
2.5.1.32 15-cis-phytoene synthaseAdvanced search results
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results (Engineering
Engineering on EC 2.5.1.32 - 15-cis-phytoene synthase
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PROTEIN VARIANTS 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
Y358F
mutant shows lower enzymatic activity than the wild-type form in a heterologous complementation assay
additional information
additional information
Citrus sp.
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recombinant in vivo reconstitution of carotinoid biosynthesis by expression of genes crtE, crtX, crtY, crtI, and crtB encoding enzymes involved in the pathway from Pantoea ananatis and citrus genes crtB and crtZ encoding CitPsy and CitChx, i.e. phytoene synthase and beta-carotene hydroxylase, in Escherichia coli strain BL21(DE3), carotinoid pigment content analysis of recombinant bacteria, overview
additional information
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the full-length overexpressed enzyme exhibits very reduced catalytic activity compared with an overexpressed N-truncated form, with its transit sequence of 214 base pairs removed from the psy cDNA at the 5'-end by site-directed mutagenesis. The shortened form readily binds to lipid bilayers
additional information
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RNA interference-based gene silencing of phytoene synthase, the OgPSY RNAi construct driven by the 35S promoter is introduced into protocorm-like body (PLB) of Oncidium orchid by Agrobacterium tumefaciens-mediated transformation
additional information
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to facilitate purification of the recombinant enzyme, the structural gene for Psase is modified by site-directed mutagenesis to incorporate a C-terminal Glu-Glu-Phe tripeptide to allow purification by immunoaffinity chromatography on an immobilized monoclonal anti-alpha-tubulin antibody YL1/2 column
additional information
overexpression in Arabidopsis thaliana enhances the growth of plants. In presence of 0.1 M NaCl, the photosynthesis rate and photosystem II activity increase by about 50%, compared to wild-type seedling. Transgenic lines display higher activities of superoxide dismutase and peroxidase and lower contents of H2O2 and malondialdehyde
additional information
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overexpression in Arabidopsis thaliana enhances the growth of plants. In presence of 0.1 M NaCl, the photosynthesis rate and photosystem II activity increase by about 50%, compared to wild-type seedling. Transgenic lines display higher activities of superoxide dismutase and peroxidase and lower contents of H2O2 and malondialdehyde
additional information
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the size of the transit peptide of phytoene synthase from ripe fruit is approximatyle 9000 Da, corresponding to about 80 amino acid residues. Removal of further N-terminal amino acids up to 114 from the enzyme, to yield a protein of apparent molecular mass 3400 Da, increases its catalytic activity in Escherichia coli
additional information
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a frameshift mutation due to a single-nucleotide insertion in codon 226 of CRTISO, which leads to an early stop codon, was found in t3002. The other alleles in the M82 variety carry missense mutations: L241K in t4838, G520R in t3406, and G546E in t9776. Expression analysis of wild-type and mutant enzymes in vivo and comparisons, overview
additional information
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a trans-splicing of PHYTOENE SYNTHASE 1 alters tomato fruit color by map-based cloning
