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2.5.1.32: 15-cis-phytoene synthase

This is an abbreviated version!
For detailed information about 15-cis-phytoene synthase, go to the full flat file.

Word Map on EC 2.5.1.32

Reaction

2 geranylgeranyl diphosphate =

15-cis-phytoene
+ 2 diphosphate

Synonyms

AtPSY, CitPsy, CrtB, crtYB, EgcrtB, fruit-specific phytoene synthase, geranylgeranyl-diphosphate geranylgeranyltransferase, MaPsy1, MaPsy2, phytoene synthase, phytoene synthase 1, phytoene synthase 2, phytoene synthase/lycopene cyclase, phytoene synthetase, phytoene-synthetase, prephytoene-diphosphate synthase, Psase, PSY, PSY protein, PSY1, PSY1a, PSY1b, PSY1C, PSY2, PSY3, PSY5, synthetase, phytoene, sZmPSY1, TaPSY1, TaPSY2, TaPSY3, YELLOW-FRUITED TOMATO 2, yft2, Zmpsy1

ECTree

     2 Transferases
         2.5 Transferring alkyl or aryl groups, other than methyl groups
             2.5.1 Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
                2.5.1.32 15-cis-phytoene synthase

Activating Compound

Activating Compound on EC 2.5.1.32 - 15-cis-phytoene synthase

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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
CTP
-
0.67 mM, 2.5fold stimulation
dithiothreitol
-
activates
galactose
-
or galactose moieties in galactolipids are required
GTP
-
0.67 mM, 2.5fold stimulation
light
light-dependent increase in carotinoid production
-
NADP+
-
weak stimulation
Triton X-100
-
0.1 w/v, 5fold stimulation
Tween 60
-
0.1 w/v, 5fold stimulation
Tween 80
-
detergent required, maximal activity at 0.08%
UTP
-
0.67 mM, 2.5fold stimulation
additional information
-
the enzyme is localized at thylakoid membranes in mature chloroplasts. Under certain light conditions, e.g. far-red light, the increase in PSY mRNA and protein levels is not accompanied by an increase in enzymatic activity. Under these conditions the enzyme is localized in the prolamellar body fraction in a mostly enzymatically inactive form. Subsequent illumination of dark-grown and/or in far-red light seedlings with white light causes the decay of these structures and a topological relocalization of PSY to developing thylakoids which results in its enzymatic activation. The light-dependent mechanism of regulation may contribute to ensuring a spatially and temporally coordinated increase in both carotenoid and chlorophyll contents
-