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capric acid
-
enhancement factor: 0.04 in absence of Triton X-100, 1.0 in presence of 0.1% Triton X-100
dipalmitoylphosphatidic acid
-
enhancement factor: 0.2 in absence of Triton X-100, 1.2 in presence of 0.1% Triton X-100
lauric acid
-
enhancement factor: 0.1 in absence of Triton X-100, 2.3 in presence of 0.1% Triton X-100
lysophosphatidylglycerol
-
weak effect in activating lipid-depleted enzyme
palmitic acid
-
enhancement factor: 0.8 in absence of Triton X-100, 1.4 in presence of 0.1% Triton X-100
phosphatidylglycerol
-
marked effect in activating lipid-depleted enzyme
phosphatidylserine
-
enhancement factor: 0.05 in absence of Triton X-100, 0.4 in presence of 0.1% Triton X-100
polyamines
-
stimulate in presence of Mg2+
-
stearic acid
-
enhancement factor: 0.07 in absence of Triton X-100, 0.8 in presence of 0.1% Triton X-100
trans,trans,trans-geranylgeranyl diphosphate
-
stimulates activity with farnesyl diphosphate as substrate
trans,trans-farnesyl diphosphate
-
stimulates activity with farnesyl diphosphate as substrate
Triton X-102
-
stimulates
-
Triton X-114
-
stimulates
Triton X-45
-
stimulates
-
additional information
-
enzyme is inactive in absence of added effectors
-
cardiolipin
-
activates
cardiolipin
-
Lactobacillus plantarum, Escherichia coli, and bovine cardiolipin. Good activator in absence of detergent
cardiolipin
-
from bovine, enhancement factor: 0.8 in absence of Triton X-100, 0.1 in presence of 0.1% Triton X-100
cardiolipin
-
weak stimulation
cardiolipin
-
poor activator
cardiolipin
-
marked effect in activating lipid-depleted enzyme
cetyl sulfate
-
activates
cetyl sulfate
-
half-maximal activation at 0.04 mM
deoxycholate
-
weak stimulation
deoxycholate
-
maximal stimulation at 0.2% v/v or 4.8 mM
deoxycholate
-
half-maximal activation at 0.33 mM
deoxycholate
-
weak stimulation
deoxycholate
-
poor activator
Dodecyl sulfate
-
activates
Dodecyl sulfate
-
half-maximal activation at 0.075 mM
lecithin
-
weak stimulation
lecithin
-
from egg, stimulates
lecithin
-
14fold stimulation at 2 mM
myristic acid
-
activates
myristic acid
-
enhancement factor: 0.2 in absence of Triton X-100, 2.1 in presence of 0.1% Triton X-100
oleic acid
-
enhancement factor: 0.9 in absence of Triton X-100, 1.3 in presence of 0.1% Triton X-100
oleic acid
-
good activator in absence of detergent
phosphatidic acid
-
from egg, good activator in absence of detergent
phosphatidic acid
-
enhancement factor: 0.8 in absence of Triton X-100, 1.0 in presence of 0.1% Triton X-100
Phospholipid
-
-
Phospholipid
-
phospholipid extract from Micrococcus luteus stimulates
Triton
-
0.01 microM of UPPs with 5 microM of (2E,6E)-farnesyl diphosphate and 50 microM of isopentenyl diphosphate, 0.5 mM MgCl2, and 50 mM KCl in Hepes buffer (pH 7.5) is 190fold less active (0.013 s-1) for the IPP consumption in the absence of Triton
Triton
-
the UPPs steady-state kcat value in the presence of 0.1% Triton is 190fold larger than in the absence of Triton
Triton X-100
-
-
Triton X-100
-
strong stimulation
Triton X-100
-
no activity in absence
Triton X-100
-
1.5% v/v, 2 molecules are bound in the active site tunnel of the beta-subunit, binding structure
Triton X-100
-
no activity in the activation by Triton X-100 is almost saturated at a concentration of 0.02%
Triton X-100
-
half-maximal activation at 3.4 mM
Triton X-100
-
requirement for Triton X-series detergents
Triton X-100
-
stimulates
Triton X-100
-
marked decrease of activity when a small amount of detergent is added, activity gradually increases as further detergent is added, being fully restored when the concentration reaches 2%
Tween 80
-
stimulates
Tween 80
-
strong stimulation