2.5.1.28: dimethylallylcistransferase
This is an abbreviated version!
For detailed information about dimethylallylcistransferase, go to the full flat file.
Word Map on EC 2.5.1.28
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2.5.1.28
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solanum
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monoterpenes
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lycopersicum
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trichomes
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tomato
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terpene
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cis-prenyltransferase
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terpenoids
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orthogonal
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d-limonene
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nerol
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rnai-mediated
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sesquiterpenes
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glandular
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olfactory
- 2.5.1.28
- solanum
- monoterpenes
- lycopersicum
- trichomes
- tomato
-
terpene
- cis-prenyltransferase
-
terpenoids
-
orthogonal
- d-limonene
- nerol
-
rnai-mediated
-
sesquiterpenes
-
glandular
-
olfactory
Reaction
Synonyms
NDPS1, neryl diphosphate synthase, neryl diphosphate synthase 1, neryl-diphosphate synthase, PsIDS3, short-chain cis-prenyldiphosphate synthase, short-chain CPT
ECTree
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Engineering
Engineering on EC 2.5.1.28 - dimethylallylcistransferase
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additional information
change of 10 residues that correlate with changes in substrate in NDPS1 from accession LA2409 to those found in zFPS, EC 2.5.1.92, from accession LA1393 (Pro/Ser-45, which corresponds to the second residue in the predicted mature protein, is excluded), and reciprocal changes in zFPS (LA1393). The two resulting recombinant proteins are named NDPS1-M10 and zFPS-M10. The NDPS1-M10 mutant variant is able to utilize dimethylallyl diphosphate and isopentenyl diphosphate to synthesize Z,Z-farnesyl diphosphate at levels that are similar to that of the zFPS enzyme from accession LA1393. The ability of mutant NDPS1-M10 to synthesize Z,Z-farnesyl diphosphate coincides with a greatly reduced ability to synthesize neryl diphosphate
additional information
constitutive and trichome-specific RNAi-mediated knockdown of NDPS1, RNAi-mediated suppression of NDPS1 with greater reductions achieved with the general 35S promoter compared to the trichome-specific MKS1 promoter, phenotypes, overview
additional information
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constitutive and trichome-specific RNAi-mediated knockdown of NDPS1, RNAi-mediated suppression of NDPS1 with greater reductions achieved with the general 35S promoter compared to the trichome-specific MKS1 promoter, phenotypes, overview
additional information
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constitutive and trichome-specific RNAi-mediated knockdown of NDPS1, RNAi-mediated suppression of NDPS1 with greater reductions achieved with the general 35S promoter compared to the trichome-specific MKS1 promoter, phenotypes, overview
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