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2.5.1.27: adenylate dimethylallyltransferase

This is an abbreviated version!
For detailed information about adenylate dimethylallyltransferase, go to the full flat file.

Word Map on EC 2.5.1.27

Reaction

dimethylallyl diphosphate
+
AMP
=
diphosphate
+
N6-(dimethylallyl)adenosine 5'-phosphate

Synonyms

2-isopentenyl-diphosphate:AMP DELTA2-isopentenyltransferase, adenosine phosphate isopentenyltransferase, adenosine phosphate-isopentenyltransferase, adenosine phosphate_isopentenyltransferase, adenylate dimethylallyltransferase, adenylate IPT, adenylate isopentenyl transferase, adenylate isopentenyltransferase, adenylate-type isopentenyl transferase, AIPT, AIPT1, AtIPT, AtIPT1, AtIPT3, AtIPT4, AtIPT5, AtIPT7, AtITP1, ATP/ADP isopentenyltransferase, cytokinin synthase, dimethylallyl-diphosphate:AMP DELTA2-isopentenyltransferase, dimethylallylpyrophosphate:5'-AMP transferase, DMA transferase, DMAPP:AMP isopentenyltransferase, DMAPP:ATP/ADP isppentenyltransferase, HlAIPT, IPT, IPT1, IPT2, IPT3, IPT5a, IPT5b, IPT6a, IPT6b, IPT9, isopentenyl transferase, isopentenyltransferase, isopentenyltransferase, adenylate, NoIPT1, OSIPT1, OSIPT3, SAG12-IPT, Tmr, Tzs

ECTree

     2 Transferases
         2.5 Transferring alkyl or aryl groups, other than methyl groups
             2.5.1 Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
                2.5.1.27 adenylate dimethylallyltransferase

Cloned

Cloned on EC 2.5.1.27 - adenylate dimethylallyltransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cDNA encoding BoAIPT1, DNA and amino acid sequence determination and analysis, sequence comparison, functional expression of N-terminally His6-tagged BoAIPT1 in Escherichia coli strain BL21 (DE3), optimum protein expression is obtained by incubating the IPTG-induced culture at 18°C for 18 hr, transgenic expression in bamboo plants, real-time PCR expression analysis
cloned and expressed in Arabidopsis thaliana under an inducible promoter
cloning from leaves, expression of IPT in transgenic peanut plants by transfection using Agrobacterium tumefaciens
-
construct consisting of the 35S promoter fused to the first intron of the maize alcohol dehydrogenase gene, fused to the isoform IPT2 coding sequence and a terminator. Ectopic expression in Arabidopsis thaliana
expressed as fusions protein in Escherichia coi and Saccharomyces cerevisiae strains CTY716 and SYY705
-
expressed in Arabidopsis thaliana
-
expressed in Arabidopsis thaliana (root, leaf and flower)
-
expressed in Escherichia coli
expressed in Escherichia coli Top10 cells
expression in Escherichia coli
fusion with a seeds-pecific lectin promoter from Glycine max and expression in Nicotiana tabacum
-
gene ipt, expression in transgenic Oryza sativa plants via Agrobacterium tumefaciens strain LBA4404 transfection using the glutenin HMW promoter
-
gene NoIPT1, DNA and amino acid sequence determination and analysis, phylogenetic analysis, functional recombinant expression of His-tagged enzyme in Escherichia coli strain BL21 (DE3) STAR
gene SAG12, overexpression in transgenic cassava plants, transgene integration patterns in SAG12-IPT transgenic lines, overview
-
overexpressed as a N-terminal hexahistidine tag protein in Escherichia coli BL21 (DE3)