2.5.1.22: spermine synthase

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For detailed information about spermine synthase, go to the full flat file.

Word Map on EC 2.5.1.22

2.5.1.22

polyamine

putrescine

s-adenosylmethionine

ornithine

aminopropyltransferases

snyder-robinson

alpha-difluoromethylornithine

acaulis5

thermospermine

trans-4-methylcyclohexylamine

gyro

kyphoscoliosis

agriculture

adometdc

2.5.1.16

medicine

Reaction

S-adenosyl 3-(methylsulfanyl)propylamine

Synonyms

ACL5, aminopropyltransferase, spermidine, OsSPMS1, SMS, spermidine aminopropyltransferase, spermine synthase, spermine synthase 1, spermine synthetase, Spm synthase, SpmS, SPMS1, SpmSyn, synthase, spermine

ECTree

2 Transferases

2.5 Transferring alkyl or aryl groups, other than methyl groups

2.5.1 Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)

2.5.1.22 spermine synthase

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Results	in table
1	Activating Compound
42	AA Sequence
5	Application
1	CAS Registry Number
12	Cloned(Commentary)
2	Cofactor
3	Crystallization (Commentary)
122	Disease/ Diagnostics
5	Expression
14	General Information
1	General Stability
4	IC50 Value
66	Inhibitors
7	Ki Value [mM]
23	KM Value [mM]
8	Localization
2	Metals/Ions
10	Molecular Weight [Da]
15	Natural Substrates/ Products (Substrates)
550	Organism
8	Pathway
14	PDB ID
6	pH Optimum
36	Protein Variants
11	Purification (Commentary)
2	Reaction
2	Reaction Type
56	Reference
65	Source Tissue
14	Specific Activity [micromol/min/mg]
1	Storage Stability
51	Substrates and Products (Substrate)
15	Subunits
37	Synonyms
1	Systematic Name
8	Temperature Optimum [°C]
10	Turnover Number [1/s]

Purification

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Purification on EC 2.5.1.22 - spermine synthase

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3 entries

Cell lysate is loaded onto a HiTrap chelating column charged with Ni2+. The enzyme is eluted with an imidazole gradient (50-250 mM) at pH 8.0. The protein is loaded onto a Superdex 200 column equilibrated with 20 mM Tris-HCl and 150 mM NaCl. Thrombin is added to combined fractions containing SpmSyn to remove the His-tag. The protein is further purified to homogeneity by ion-exchange chromatography.

Homo sapiens

P52788

692270

His GraviTrap column chromatography, and gel filtration

partial

spermine-Sepharose affinity chromatography

Bos taurus

489863, 489874

TALON affinity resin column chromatography

2 entries