2.5.1.22: spermine synthase

This is an abbreviated version!
For detailed information about spermine synthase, go to the full flat file.

Reaction

Synonyms

ACL5, aminopropyltransferase, spermidine, OsSPMS1, SMS, spermidine aminopropyltransferase, spermine synthase, spermine synthase 1, spermine synthetase, Spm synthase, SpmS, SPMS1, SpmSyn, synthase, spermine

ECTree

     2 Transferases

         2.5 Transferring alkyl or aryl groups, other than methyl groups

             2.5.1 Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)

                2.5.1.22 spermine synthase

Advanced search results

Do not include text mining results

Include results (more...)

Include results (more...)

Results	in table
1	Activating Compound
42	AA Sequence
5	Application
1	CAS Registry Number
12	Cloned(Commentary)
2	Cofactor
3	Crystallization (Commentary)
122	Disease/ Diagnostics
5	Expression
14	General Information
1	General Stability
4	IC50 Value
66	Inhibitors
7	Ki Value [mM]
23	KM Value [mM]
8	Localization
2	Metals/Ions
10	Molecular Weight [Da]
15	Natural Substrates/ Products (Substrates)
550	Organism
8	Pathway
14	PDB ID
6	pH Optimum
36	Protein Variants
11	Purification (Commentary)
2	Reaction
2	Reaction Type
56	Reference
65	Source Tissue
14	Specific Activity [micromol/min/mg]
1	Storage Stability
51	Substrates and Products (Substrate)
15	Subunits
37	Synonyms
1	Systematic Name
8	Temperature Optimum [°C]
10	Turnover Number [1/s]

Cloned

print visible entries

print all entries

Go back to the abbreviated version
of the Enzyme Summary Page.

Cloned on EC 2.5.1.22 - spermine synthase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.

top print hide Go to Cloned (commentary) Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

CLONED (Commentary)

ORGANISM

UNIPROT

LITERATURE

-

2 entries

DNA fragment encoding human enzyme is amplified by PCR and subcloned into the pET28a-LIC vector downstream of the polyhistidine coding region. Enzyme is expressed in the Escherichia coli BL21-Codon Plus(DE3)-RIL strain by the addition of 1 mM isopropyl1-thio-beta-D-galactopyranoside. The N-terminally truncated SpmSyn mutants are generated by PCR and subcloned into the pET28a-LIC vector.

Homo sapiens

P52788

692270

DNA sequence determination and analysis of gene SPMS, construction of expression vectors for recombinant expression in transgenic plants/mutants of genes ACAULIS5, i.e. ACL5, and SPMS

Arabidopsis thaliana

-

658727

enzyme overexpression in transgenic mice under control of a composite CMV-IE enhancer-chicken beta-actin promotor, 4 separate founder CAG/SpmS mice are analysed: enzyme expression in all tissues, mostly highly increased compared to the wild-type mice, overview

Homo sapiens

-

657842

expressed in a yeast mutant deficient in Spm synthase, and in the Arabidopsis dwarf mutant acl5

Malus sylvestris

-

673827

expressed in Escherichia coli

Zea mays

-

739338

expressed in Escherichia coli BL21(DE3) cells

Gentiana triflora

A0A0K2S254

737608

expressed in Escherichia coli XL-1 Blue cells

Homo sapiens

-

739393

expressed in Nicotiana tabacum leaves

Camellia sinensis

A0A0E3DGE7

737476

expression of human spermine synthase in CAGSMS line 8 mice from a composite CMV-IE enhancer/chicken beta-actin promoter. Transgenic expression of spermine synthase in the Gy mice reverse all of the increases in dcAdoMet content and AdoMetDC

Homo sapiens

-

721528

expression of wild-type and mutant enzymes in HEK cells

Homo sapiens

P52788

723531

-

Homo sapiens

-

489879

-

Homo sapiens

P52788

758944